( )

EXTRACTION AND DETERMINATION OF FRUCTANS

(OLIGOFRUCTOSES AND INULIN) IN MEDICINAL PLANTS
NADEZHDA PETKOVA, PANTELEY DENEV
,
, . 26,
, 4002
E-mail: petkovanadejda@abv.bg, denev57@abv.bg
Abstract
he continuous ethanol and water extractions of the dry roots from medicinal
plants and Helianthus tuberosus tubers were carried out. The amount of extracted
fructans was defined by the resorcinol method. The obtained average fructan
content was as follows: Cichorium intybus - 33 %, Inula helenium - 42-44 % and
Taraxacum officinale 34 % and Helianthus tuberosus 69 %, which make them
rich sources of soluble dietary fiber, when they were used in dietary food products.

(Inula helenium L.), (Cichorium

intybus L.), (Taraxacum officinale Web.),
(Helianthus tuberosus L.) . Compositae (Asteraceae).

, - [2, 4, 8,
9].
.

-(21)
(Fm), ,
- (GFn) (.1) [9, 12].
(DP) 2 70. (
) DP 2 12 [7,
11, 12].
. ,
- ,

. ,

399

 .
,
Bifidobacteria, (SCFA),
[6, 11].
,
[5].
,
,
. ,
, [7].

.
-
-
.
HO

OH
O

HO

H2C
OH

HO

OH
OH
H2C

HO
O

H 2C

HO

CH2
O

HO
HO

H2C

OH

HO

CH2

CH2

HO

OH

H 2C

H2C

OH

CH2

HO

H2C

HO
HO

CH2

OH

H2C

OH

HO
O

OH

OH

CH2

HO

HO

CH2

HO

OH
O

CH2

OH

H2C

H 2C

OH

OH

GFn
Fm
.1

(Inula helenium)
2010 . 2011 .
.
. , . 2011 .,
(Cichorium intybus) 2011 .
,
1 mm.
,
[10].
AOAC 945.32
105 C [3].


. 0,45
g () , 40

400

ml 95 %- 60 min
.
0,45 m
, 40 20 ml .
100 ml .

40, 40 20 ml . H2O.
60 min, .
100 ml .H2O.


(TLC). TLC Kieselgel 60 F254 (Merck, Germany) 5 l
. 5
l , , (Sigma Aldrich), (Frutafit CLR
DP 7-9 Frutafit HD DP 9-12), (Frutafit TEX, DP 22),
2 mg/ml.
: 1) n-BuOH:EtOH:H2O (5:3:2) (v/v)

---H3PO4
[11];
2)
n-BuOH:iPrOH:H2O:CH3COOH (7:5:4:2) -H3PO4 (1:1:5:50) [9].
.

,
. -
,
, 480 nm
[2]. 0,1 ml ,
, 0,9 ml k.HCl 0,8 ml 95 % .

(1).

:
c , g/ml;
Ve , ml;
r , r = 100;
kw (

);

w , %
0,91 - ;
p , g;
6
10 g g.

Microsoft Excel 2010.

401




( 1). 10 %,
- .
1


, %
CV*, %
Inula helenium L. ( ).
8,50
0,15
Cichorium intybus L. ( , )
7,76
0,24
Taraxacum ollicinale Weber ()
8,46
0,12
Helianthus tuberosus L.
9,66
0,19
(, , )
*CV , %
TLC

(. 2). Helianthus tuberosus
. ,
(.2 )),
.
(Taraxacum officinale)

,
, DP
22. e Inula helenium
(.2 ) Cichorium intybus.
) Helianthus tuberosus

) Inula helenium

) Taraxacum ollicinale

. 2. LC () (W)
) ) , n-BuOH:i-PrOH:H2O:CH3COOH (7:5:4:2)
--H3PO4 (1:1:5:50); )
n-BuOH:EtOH:H2O (5:3:2) (v/v) --H3PO4,;

402

 ,
.
. -
: nBuOH:i-PrOH:H2O:CH3COOH (7:5:4:2).

, ,
.
( 2) ,

- (50-70 %),
- 2010 . -
(41-43 %)
.
Cichorium intybus L var sativum (33 %),
(witloof), .
2

Composit (Asteraceae).

%, .

(
(),
*), %,
%, .
*
.**
Inula

10,92,2
32,02,0
42,9
helenium L
2011
Inula

8,3 1,5
32,70,5
41,0
helenium L
2010
Cichorium

16,10,1
intybus L.

12,12,5
21,2 1,5
33,3
2011
Taraxacum

10,70,7
23,94,2
34,6
officinale
2011
Helianthus

6,4 0,4
43,52,5
49,9
tuberosus
2011
Helianthus
2010

45,42,2
23,91,0
69,3
tuberosus
* ()
**..

TLC , Helianthus
tuberosus e - (50-70 %
. ), .

403

 Taraxacum officinale
, Cichorium intybus
Inula helenium
.

,
,

Bifidobacteria ().

1. , . .; , . . , , . ., .
.;
(Inula helenium L.)
; 2008, 1, . 9599
2. , ., , ., , ., , .,
,
, 2011, .
38, . 5, . 47-55
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Artichoke
(Helianthus
tuberosus L.),
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