Chloramphenicol

01. Nov. 2011, 12:47:02, Spline, 450 nm, Ser.No: 10507,128, Version: 1.56
Plate Values

A
B
C
D
E
F
G
H

10

11

12

1.986
1.689
1.356
0.958
0.656
0.332
1.998
1.982

1.239
1.982
1.985
1.962
1.763
1.789
1.780
0.000

0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000

0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000

0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000

0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000

0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000

0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000

0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000

0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000

0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000

0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000

100

Standards

Standard curve

Conc.(ppb) / A(Mean)

90

A
b
s
o
r
b
a
n
c
e
(%)

80

0.050
1.689
(Coeff. of Var.: 0.0%)

70

0.150
1.356
(Coeff. of Var.: 0.0%)

60

0.450
0.958
(Coeff. of Var.: 0.0%)

50

1.4
0.656
(Coeff. of Var.: 0.0%)

40

4.1
0.332
(Coeff. of Var.: 0.0%)

30
20
10
0
0.050

0.150

0.450

1.4

4.0

Concentration (ppb)

50% inhibition: 0.405

points of inflection: 3

C:\RIDAWIN\FOOD\ANTIBIO\CAP1.MET, D:\AEKHAN~1\ANTI\CAP\RAW\CAP2011\THANG11\0111MES.MES
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Chloramphenicol
01. Nov. 2011, 12:47:02, Spline, 450 nm, Ser.No: 10507,128, Version: 1.56
Standards
Ser. No.

Concentration
ppb

Absorbance
(Mean)
(CV)

B/B0
(%)

calculated
ppb

Deviation
(%)

0.00

1.986

0.0

100.0

0.050

1.689

0.0

0.150

1.356

0.0

85.0

0.050

0.0

68.3

0.150

0.0

0.450

0.958

0.0

48.2

0.451

0.2

1.35

0.656

0.0

33.0

1.35

0.0

4.05

0.332

0.0

16.7

4.05

0.0

Samples
Ser. No.

ID

Absorbance
(Mean) (CV)
(%)

calculated
ppb

REABLANK

1.998

0.0

100.6

not calculable!

0.50

SAMBLANK

1.982

0.0

99.8

< 0.050 ppb

0.50

SPK0.1ppb

1.239

0.0

62.4

0.206

0.50

RM 111012011 VN

1.982

0.0

99.8

< 0.050 ppb

0.50

RM 111012021 VN

1.985

0.0

99.9

< 0.050 ppb

0.50

RM 111012031 VN

1.962

0.0

98.8

< 0.050 ppb

0.50

RM 111012041 VN

1.763

0.0

88.8

< 0.050 ppb

0.50

RM 111012051 VN

1.789

0.0

90.1

< 0.050 ppb

0.50

FG 110312018100

1.780

0.0

89.6

< 0.050 ppb

0.50

C:\RIDAWIN\FOOD\ANTIBIO\CAP1.MET, D:\AEKHAN~1\ANTI\CAP\RAW\CAP2011\THANG11\0111MES.MES
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ppb

0.103

Chloramphenicol
01. Nov. 2011, 12:47:02, Spline, 450 nm, Ser.No: 10507,128, Version: 1.56
Method

Properties
Name
: Chloramphenicol
Dimension : ppb
Calculation : Spline

Standards
Amount
:6
Replicates : 1,1,1,1,1,1
Conc.
: 0.0,0.050,0.150,0.450,1.4,4.1

Samples
Amount

:0

C:\RIDAWIN\FOOD\ANTIBIO\CAP1.MET, D:\AEKHAN~1\ANTI\CAP\RAW\CAP2011\THANG11\0111MES.MES
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