Edexcel GCE Biology AS Unit 3 !

!You can investigate the effect of caffeine on the heart rate of Daphnia Daphnia are tiny aquatic invertebrates. They're transparent, so you can see their internal organs. This means it's pretty easy to monitor their heart rate (the number of heartbeats in a minute) by observing them through a microscope. Here's how you could investigate the effect of :caffeine on their heart rate Make up a range of caffeine solutions of different concentrations and (1 .a control solution that has no caffeine in it at all Transfer one Daphnia into the dimple on a cavity slide (a microscope (2 .(slide with a rounded dip Place the slide onto the stage of a light microscope and focus it on the (3 .beating heart of the Daphnia .Place a small drop of caffeine solution onto the Daphnia (4 Count the number of heartbeats in 10 seconds and multiply this by six (5 .(to calculate beats per minute (heart rate Repeat this 10 times using the same concentration of caffeine but a (6 .different Daphnia individual each time Don't forget to keep all other factors constant (e.g. temperature and (7 .(volume of caffeine solution Repeat the experiment using the other concentrations of caffeine (8 .solution
heart rate / bpm

Calculate the average reading for each concentration and draw a (9 .graph of the results
.Caffeine conc

The graph should show a positive correlation as caffeine (10 .concentration increases, heart rate also increases

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