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Histolo gy

Histolo gy
Laszlo Vass, Ed.D. 42-0013-00-01 Version

L ab RepoRt assistant
This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiments questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students writing of lab reports by providing this information in an editable file which can be sent to an instructor.

Purpo se
What is the purpose of this exercise? Are there any safety concerns associated with this exercise? If so, list what they are and what precautions should be taken.

Exercise Epithelial Tissue

1:

Data Table 1: Epithelial Tissue Observations

TISSUE TYPE Simple Squamous Simple Cuboidal Simple Columnar (stomach) Simple Columnar (duodenum) OBSERVATIONS
Alveoli, simple squamous cells Basement membrane, connective tissues, simple cuboidal cells Basement membrane, connective tissue, simple columnar cells, microvilli, nuclei Basement membrane, connective tissue, simple columnar cells, microvilli, nuclei Keratinized cells, epidermis, papillary

Stratified Squamous (keratinized) dermis, reticular dermis Stratified Squamous (nonkeratinized)

Stratified squamous cells, basement membrane, connective tissue Cilia, psuedostratified cells, basement

Pseudostratified Ciliated Columnarmembrane, connective tissue

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Transitional

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Umbrella cells, basement membrane, connective tissue Stratified cuboidal cells, connective tissue, sweat gland Stratified columnar calls, cell nuclei, connective tissue

Stratified Cuboidal (online) Stratified Columnar (online)

www.LabPaq.

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Questions
A. Why is the study of histology important in the overall understanding of anatomy and physiology?
it helps systems. The study of histology is important in the overall understanding of anatomy and physiology because

with understanding the structure and function of various tissues is important for studying organs and

B. How are epithelial tissues named?

Epithelial tissues are named based on shape and arrangement.

C. Why are some epithelial tissues stratified?

simple tissues which are only a single layer of cells.

Some epithelial tissues are stratified because they have multiple layers of cells as opposed to

D. Unlike squamous cells, cuboidal and columnar cells have large, open cytoplasm. Which functions of epithelial tissue are supported by having such big cells?
Absorption, secretion and it allows rapid diffusion or transport of substances through membrane.

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E.

Histolo gy

Look at the following drawings and identify each type of epithelial tissue:

1. simple squamous

2. simple columnar

3. pseudostrati fied columnar

4. stratified squamous

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Exercise 2: Connective Tissue

obseRvations
Data Table 2: Connective Tissue
Tissue
Mesenchyme (online) Aerolar (online) Adipose (online) Dense Irregular (online) Reticular Dense Regular: Tendon Hyaline Cartilage Elastic Cartilage Fibrocartilage Compact Bone Human Blood

Amount and Shape of Cells 23polygonal

28- spindle 8- polygonal 20- polygonal 40- star shaped Too many to count Too many to countround Too many to count Too many to count Too many to countround Too many to countround

Amount of Matrix 20%

30% N/A 35% 30% N/A 30% 50% 40% 90%

Both

Are there fibers? If so, are they parallel

Parallel N/A Scattered Scattered Parallel N/A Scattered Parallel Parallel

Too much

N/A

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Questions
A. What is the primary function of connective tissue?
They protect, support and bind together the other tissues of the body.

B. What can the shape of the cells in a particular type of tissue tell about the function of that tissue?
dimensional from its

Some shapes refer to the way a cell looks in typical tissue sections, not to the complete three-

shape of the cell. Cells that look one way in a tissue section usually look completely different if viewed upper surface.

C. What is matrix? Why do some tissues have more matrix than others?
Matrix is non-cellular matter between the cells. Some tissues have more matrix than others because the cells make and extrude the matrix that surrounds them.

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D. What do collagen fibers provide?

Collagen fibers allow tissues to be flexible while providing additional strength and stability.

E. Tendons, ligaments and cartilage have limited blood supply. Explain how this might affect the ability of these tissues to heal after an injury.
It limits the rate at which mitosis can occur.

Exercise 3: Muscle Tissue

obseRvations
Data Table 3: Muscle Tissue
Muscl Shape and Arrangement of Cells e Skelet al Smoot h Cardia c
Skeletal muscle cells, nuclei

Str ia tions Present? Yes

No No

Endomysium, cell nuclei, smooth muscle cell Cell nuclei, intercalated discs, cardiac muscle cell

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Questions

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A. What kind of muscle would you find in the stomach?

Smooth muscle would be found in the stomach.

B. How is smooth muscle structure different from that of skeletal and cardiac muscle? Smooth muscle lacks striations and is involuntary. C. Why is skeletal muscle voluntary? It is under voluntary control from the brain and provides strength to move the limbs and body.

D. What is unique about cardiac muscle?

electrical Cardiac muscle is composed of a series of cells that work together as one unit and respond to impulses that allow the heart to beat.

Exercise 4: Nervous Tissue

obseRvations
Make a sketch of the neuron as seen in your microscope slide and label the following: Cell body, nucleus, cell processes and neuroglia.

Questions
A. What is the function of nervous tissue?
changes in the environment or body. Nervous tissue is specialized to send electrical signals that inform the central nervous system of

B. Why are the cell bodies of neurons elongated into cell processes?
For transmission of information. These dendrites receive signals from other cells and conduct messages to the cell body.

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C. If all nerves respond to stimuli, why cannot eyes hear sound and ears see light?
Each nerve is designed to respond to and transmit specific stimuli from a certain area of the body.

D. How is a nerve different from a neuron?

A neuron is only one part of a nerce. A neuron and neuroglia together make up a complete nerve.

Conclusions
Explain the purpose of these exercises and why studying histology is important to the understanding of how the human body functions. Histology allowed us to examine the structure and composition of all of our tissues and how they are arranged into organs.

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