6-FAM

for Proligo® Reagents

Product Information

SAFC Supply Solutions® is extending its Proligo® Reagents offer with

6-FAM as a common fluorescent derivatization reagent for covalent
labeling of synthetic oligonucleotides.

Figure A. 6-FAM

O O O

O 1 O
O2
7
N
H 3 O
NC P N
O O 6 4
5
O

Key Features of 6-FAM

• 6-FAM consists of one isomer derived • The labeled oligonucleotide is ready

from 6-carboxy fluorescein for use in most applications after
the evaporation of ammonia. Standard
• Couples to the 5’-end of the
procedures can be used if additional
oligonucleotide using standard
purification is required.
synthesis protocols
• Lot to lot consistent purity and
• Protecting groups on the fluorescein
performance
moiety are removed under standard
conditions of cleavage and deprotection • Manufactured under a certified
with concentrated ammonia ISO 9000:2000 quality system

• A detritylation step is not required since

the fluorescein phosphoramidite does
not contain a DMT-group

Ordering Information Analytical specifications of 6-FAM

Description Unit Cat. No. Test 6-FAM
Compatible with ExpediteTM and Polygen® Instruments HPLC Purity ≥ 97.0%
6-FAM 1 x 0.1g X010181-01 P-NMR
31
≥ 97.0%
Compatible with ABI® Instruments
6-FAM 1 x 0.1g X010131-01

For other quantities and custom packaging requirements

please contact your sales representative to inquire. Some
packaging options may not be available in your region.
Sigma-Aldrich Biochemie GmbH
Georg-Heyken-Strasse 14
21147 Hamburg, Germany
+49 40 797 02 250 (Europe)
+1 877 776 5446 (US Toll Free)
Global Email: safcproligoreagents@sial.com

SAFC®, SAFC Supply Solutions®, Sigma-Aldrich® and SAFC Proligo® Reagents

are registered trademarks of Sigma-Aldrich Biotechnology L.P. and
Sigma-Aldrich Co. ABI® is a registered trademark of Applera Corporation or its
subsidiaries. Expedite™ is a trademark of Applera Corporation or its
subsidiaries. PolyGen® is a registered trademark of PolyGen GmbH.
© 2009 SAFC All rights reserved.

LNB
05424-503120
0049 www.safcsupplysolutions.com/newproducts