Research Journal of Chemistry and Environment _________________________________Vol.16 (4) December (2012) Res.J.Chem.

Environ

Development and validation of HPLC method for the determination of amygdalin in the plant extract of plum kernel
Savic Ivan M.,* Nikolic Vesna D., Savic Ivana M., Nikolic Ljubisa B. and Stankovic Mihajlo Z.
Faculty of Technology, University of Nis, Bulevar oslobodjenja 124, 16000, Leskovac, SERBIA *ici_teh@yahoo.com

Abstract A HPLC method was developed, validated and applied for the determination of amygdalin in the plant extract of plum kernel. The effects of mobile phase composition, stationary phase types and organic modifiers on retention factors of amygdalin were investigated. The chromatographic column used was 5 m SUPEL-CO Analytical HSC18 column (4.6250mm), the mobile phase being water: acetonitrile (25:75v/v)), held at 25C. Analysis was run at a flow-rate of 0.9cm3min1 and at a detection wavelength of 215nm. The results showed that the peak area responses are linear within the concentration range of 10-100g cm3 (r=0.9986). The method yielded good results with a quantitation limit of 3.49gcm-3 and a detection limit of 1.06gcm-3. The intra-day and intrainstrument reproducibility, RSD were 0.07-0.78 and 0.85-1.62 respectively. The accuracy results of 80%, 100% and 120% amygdalin were 102.8%, 97.74% and 98.36%, respectively. The method is linear, quantitative and reproducible and could be used as a more convenient, efficient and economical method for the trace analysis of amygdalin in the plant extract of plum kernel.

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