Você está na página 1de 1

Power Supplies

Bio-Rad offers a complete line of power supplies for the most demanding of electrophoretic techniques. These power supplies are
lightweight and easy to program, and are certified to IEC 1010-1 — the most rigorous international safety standard — to ensure the highest
personal and environmental protection. Use the selection guide to choose the power supply that best suits your electrophoresis needs.
Power Supply Selection Guide According to Electrophoretic Technique
Technique and Typical PowerPac™
Recommended Gel or Tube Size, Qty Typical Conditions* (Initial) Typical Conditions* (Final) Run Power
Apparatus (W x L x Thickness) W V mA W V mA Time Supply
Laemmli (SDS), O’Farrell Second Dimension (SDS)
PROTEAN® II xi cell 160 x 160 x 1.5 mm, 2 gels — 100 35(C) — 350 35(C) 5 hr HV or Universal
PROTEAN II XL cell 183 x 200 x 1.5 mm, 2 gels — 100 35(C) — 350 35(C) 5 hr HV or Universal
Criterion™ cell 133 x 87 x 1.0 mm, 2 gels — 200(C) 200 — 200(C) 80 55 min Basic or HC
Mini-PROTEAN ®
83 x 73 x 1.0 mm, 4 gels — 200(C) 120 — 200(C) 60 45 min Basic or HC
Tetra cell
High-Throughput Electrophoresis
PROTEAN® Plus 200 x 205 x 1.0 mm, 12 gels — 200(C) 1,000 — 200(C) 350 6 hr HC or Universal
Dodeca™ cell
PROTEAN II xi/XL 160 x 200 x 1.5 mm, 6 gels — 150 480(C) — 500 480(C) 5 hr Universal
multi-cell
Criterion Dodeca cell 133 x 87 x 1.0 mm, 12 gels — 200(C) 1,000–1,400 — 200(C) 400–500 55 min HC or Universal
Mini-PROTEAN 3 83 x 73 x 1.0 mm, 12 gels — 200(C) 600 — 200(C) 360 45 min HC or Universal
Dodeca cell
Isoelectric Focusing (IEF), O’Farrell First Dimension
PROTEAN II xi cells 150 x 1.5 mm tubes, — 800(C) 3.5 — 800(C) <1 16 hr HV or Universal
or Model 175 tube cell 4 (minimum)
Mini-PROTEAN II 75 x 1.0 mm tubes, — 750(C) 1 — 750(C) <1 3–4 hr HV or Universal
tube cell 8 (minimum)
Preparative Electrofocusing
Rotofor® cell 60 ml focusing chamber 15(C) 500 24 15(C) 1,200 10 4 hr HV
Mini Rotofor cell 18 ml focusing chamber 12(C) 500 24 12(C) 1,200 10 4 hr HV
Preparative PAGE
Model 491 prep cell — 10(C) 300 40 10(C) 400 30 3–8 hr HV or Universal
Mini prep cell — 1(C) 200 5 1(C) 300 3 3–8 hr HV or Universal
Protein Electroelution
Model 422 6 samples — 200 60(C) — 150 60(C) 3–4 hr Basic, HV,
electro-eluter or Universal
Whole gel eluter 160 x 140 mm or larger 15 200(C) — 15 200(C) — 20 min Basic
Mini whole gel eluter 55 x 65 mm or larger 10 100(C) — 10 100(C) — 20 min Basic
Polyacrylamide Analytical Electrofocusing
Model 111 mini IEF cell 125 x 65 x 0.4 mm — 100(C) 6 — 100(C) 4 15 min HV or Universal
— 200(C) 6 — 200(C) 4 15 min
— 450(C) 4 — 450(C) 1 1 hr
DNA Restriction Analysis (Horizontal Mode)
Sub-Cell® GT cell 150 x 200 x 5 mm — 80(C) 55 — 80(C) 60 4 hr Basic or HC
Mini-Sub® Cell GT 70 x 100 x 5 mm — 50(C) 25 — 50(C) 30 2 hr Basic
Wide Mini-Sub Cell GT 150 x 100 x 5 mm — 50(C) 35 — 50(C) 40 2 hr Basic
DNA Sequencing
Sequi-Gen® GT system 380 x 500 x 0.4 mm 80(C) 1,850 30 80(C) 1,850 30 2–4 hr HV
Single-Stranded Conformation Polymorphism (SSCP)
Sequi-Gen GT system 210 x 400 x 0.4 mm 40(C) 1,800 20 40(C) 1,800 20 2–3 hr HV
Microsatellite Mapping
Sequi-Gen GT system 210 x 400 x 0.4 mm 50(C) 2,100 25 50(C) 2,100 25 2–3 hr HV
Mutation Detection
DCode™ system 100 x 75 x 1.0 mm, 2 gels — 130(C) — — 130(C) — 2.5 hr Basic or HV
Western Blotting
Mini Trans-Blot® cell 83 x 73 x 0.75 mm, 2 gels — 100(C) 250 — 100(C) 450 1 hr HC
Criterion blotter
Wire electrodes 133 x 87 x 1.0 mm, 2 gels — 100(C) 250 — 100(C) 450 1 hr HC
Plate electrodes 133 x 87 x 1.0 mm, 2 gels — 100(C) 650 — 100(C) 1,600 30 min HC
Trans-Blot® cell
Wire electrodes 200 x 160 x 1.5 mm, 1 gel — 60(C) 210–250 — 60(C) 210–250 5 hr HC
Plate electrodes 200 x 160 x 1.5 mm, 1 gel — 100–150(C) 1,000–1,600 — 100–150(C) 1,000–1,600 1–5 hr HC
High-intensity transfer 200 x 160 x 1.5 mm, 1 gel — 50–100(C) 1,600 — 50–100(C) 1,600 30 min HC
Trans-Blot® Plus cell 265 x 280 x 1.5 mm, 3 gels — 100(C) 3,000 — 100(C) 3,000 30 min–1 hr HC
Semi-Dry Blotting
Trans-Blot SD cell
Protein 250 x 180 x 1.5 mm — 15(C) 500 — 15(C) 200 15–30 min HC
DNA/RNA 150 x 150 x 6 mm — 15 650(C) — 25 650(C) 10–30 min HC
* (C) = constant; typical conditions are listed as guidelines only and can vary based on sample, buffers, etc.

Você também pode gostar