PENGARUH PEMBERIAN METANOL DAN

ETANOLTERHADAP TINGKAT KERUSAKAN SEL HEPAR

TIKUS WISTAR
THE EFFECT OF METHANOL AND ETHANOL ADMINISTRATION TO
LIVER CELL DAMAGE IN WISTAR RATS
ARTIKEL
KARYA TULIS ILMIAH
Disusun untuk memenuhi sebaian !e"s#a"atan una men$a!ai %e"a&at
st"ata'( ke%)kte"an umum
NORMA NABILA
G*A++,(--
PROGRAM PENDIDIKAN SAR.ANA KEDOKTERAN
/AKULTAS KEDOKTERAN
UNI0ERSITAS DIPONEGORO
TAHUN *+((
1
PENGARUH PEMBERIAN METANOL DAN ETANOL TERHADAP
TINGKAT KERUSAKAN SEL HATI TIKUS WISTAR
Norma Nabila
1
, Santosa
2
ABSTRAK
Lata" be1akan 2 Mtanol biasa !i"#na$an sba"ai %lar#t or"ani$, mr#%a$an
&nis al$o'ol (an" mm%#n(ai str#$t#r %alin" s!r'ana, tta%i san"at to$si$ %a!a
man#sia) *ra+#nan mtanol mn"a$ibat$an asi!osis mtaboli$, $om%li$asi
n#rolo"is ba'$an $matian)

Mtabolism mtanol !an tanol sba"ian bsar
tr&a!i !i '%ar !an mmili$i %otnsi mr#sa$ sl '%ar) ,an(a$ %nlitian (an"
mnliti tntan" into$si$asi mtanol !an tanol %a!a sl '%ar tta%i %nlitian
mn"nai %n"ar#' $r#sa$an s+ara 'isto%atolo"i bl#m %rna' !itm#$an)
-nlitian ini brt#&#an #nt#$ mn"ta'#i %n"ar#' %mbrian mtanol !an
tanol tr'a!a% tin"$at $r#sa$an sl '%ar s+ara 'isto%atolo"i %a!a ti$#s Wistar)
Met)%e 2 -nlitian $s%rimntal !n"an ran+an"an post test only controlled
group design) Sam%l br#%a 2. $or ti$#s Wistar &antan (an" !iba"i mn&a!i 1
$lom%o$ $ontrol !an / $lom%o$ %rla$#an) -rla$#an !ibri$an mlal#i son!
!n"an !osis mtanol 0 ml1$" brat ba!an %r oral !an !osis tanol 2 "r1$" brat
ba!an %r oral) * ti!a$ !ibri$an mtanol ata#%#n tanol, -1 !ibri mtanol, -2
!ibri tanol, !an -/ !ibri mtanol !an tanol) -rla$#an !ibri$an slama 13
'ari) Stla' %mbrian %rla$#an, ti$#s !i!$a%itasi, !ia#to%si #nt#$
%n"ambilan ota$, !an !ib#at %r%arat 'isto%atolo"is)
Hasi1 2 Hasil %nlitian !ianalisa mn""#na$an #&i One-way Annova !i!a%at$an
%4.,...) Dilan&#t$an !n"an #&i Post Hoc, !i%rol' ba'5a tr!a%at %rb!aan
(an" brma$na antara *6-17p4.,..28, *6-27p4.,..38, *6-/7p4.,...8, -16
-/7p4.,.298, -26-/ 7p4.,.218)
Sim!u1an 2 Mtanol !an tanol :$ mr#sa$ sl '%ar (an" 'am%ir sama tta%i
ti!a$ brma$na) Nam#n, bila !ibri$an brsamaan, mtanol !an tanol mmili$i
:$ sinr"isti$, !imana $!#a sn(a5a mnin"$at$an $r#sa$an sl '%ar s+ara
brma$na bila !iban!in"$an !n"an $lom%o$ %rla$#an lain3
Kata kun$i 2 mtanol, tanol, ti$#s Wistar
1
Ma'asis5a %ro"ram %n!i!i$an S61 $!o$tran #m#m F* ;n!i%
2
Sta: %n"a&ar ,a"ian *!o$tran Fornsi$ F* ;n!i%, <l Dr) S#tomo No) 12
Smaran"
2
THE E//E4T O/ METHANOL AND ETHANOL ADMINISTRATION TO
LI0ER 4ELL DAMAGE IN WISTAR RATS
ABSTRA4T
Background : Methanol usually used for organic solvent is a kind of alcohol that
has the most simple structure but very toxic to human. Methanol intoxication can
lead to acidosis metabolic neurological complication even death. Methanol and
ethanol is metabolised mostly in liver and have potential to damage liver cell.
!here are lot of study about the effect of methanol and ethanol intoxication on
liver cell but the study about the damage with microscopic analysis has not yet
been found . !his study aims to determine the histopathological effect of methanol
and ethanol administration to liver cell damage in "istar rat)
Method : #xperimental research with post test only controlled group design. Our
sampel is $% male "istar rats that devided by one control and three treatment
group. !reatment was gived through sonde with & ml'kg body weight per oral
dose of methanol (gr'kg body weight dose of taurine. ) group not recieved any
treatment. P* group recieved ethanol treatment. P$ recieved methanol treatment.
P+ group recieved methanol and ethanol treatment. All the treatment is given for
*, days. After the treatment the rats was decapitated autopsied the brain organ
for preparation to make histopathology sampel.
Result : -ata result is analysed by One-way Annova test showed p.%%%%.
/ontinued by Post Hoc test showed that there is a significant differences between
)-P*0p.%%%$1 )-P$0p.%%%,1 )-P+0p.%%%%1 P*-P+0p.%%$21 P$-P+
0p.%%$*1.
Conslusion : Methanol and ethanol have almost same destruction effect in the
liver cell but not significant. However if they gived simultaneously methanol and
ethanol have synergistic effect that both compound increased more damage in
liver cell significantly compare to other treatment group
Keywords : methanol ethanol wistar rat
3
PENDAHULUAN
Dari ta'#n $ ta'#n $as#s min#man o%losan srin" tr&a!i, tr+atat sam%ai
= :br#ari 2.11 10 oran" $orban mnin""al !an 192 $orban lain(a !i ra5at !i
r#ma' sa$it a$ibat min#man o%losan !an !#a !iantara $orban mnin""al
mr#%a$an 5ar"a n"ara asin")
1,9,1.
) Cam%#ran (an" !i"#na$an sba"ai min#man
o%losan brma+am6ma+am, sala' sat# !iantaran(a (ait# mtanol, mtanol srin"
!i"#na$an sba"ai +am%#ran min#man o%losan !i$arna$an 'ar"a mtanol (an"
rlati: lbi' m#ra', %ro!#$ s%rti ini !isb#t al$o'ol !nat#rasi)
=
Mtanol biasa !i"#na$an sba"ai %lar#t or"ani$, mr#%a$an &nis al$o'ol
(an" mm%#n(ai str#$t#r %alin" s!r'ana, tta%i %alin" to$si$ %a!a man#sia)
*ra+#nan a$ibat mtanol biasan(a tr&a!i $arna o>r!osis (an" s+ara sn"a&a
ata# ti!a$ sn"a&a trtlan s'in""a mn(bab$an asi!osis mtaboli$)
?
Mtabolism mtanol sba"ian bsar tr&a!i !i '%ar, $arna it# sala' sat# or"an
(an" mn"alami $r#sa$an a$ibat mtanol a!ala' '%ar) *r#sa$an %a!a sl
'%ar !isbab$an $arna ra!i$al bbas, :ormal!'i! !an asam :ormat)
Formal!'i! mnin"$at$an li%i! %ro$si!as (an" !a%at mn"a$ibat$an
$r#sa$an sl mmbran !an $matian sl)
2
Asam :ormat mn"'ambat a$ti:itas
o$si!asi mito$on!rial sito$rom, mn"'alan"i mtabolism o$si!ati: !an
mn"a$ibat$an 'i%o$sia &arin"an)
22
Etanol, !isb#t &#"a til al$o'ol, al$o'ol m#rni,al$o'ol absol#t ata#
alkohol sa&a,a!ala' s&nis +airan (an" m#!a' mn"#a%, m#!a' trba$ar, ta$
br5arna, !an mr#%a$an al$o'ol (an" %alin" srin" !i"#na$an !alam $'i!#%an
s'ari6'ari)
=
Mtabolism tanol sba"ian bsar tr&a!i !i '%ar, -a!a %n""#naan
tanol !alam &#mla' (an" bsar ata# !alam &an"$a 5a$t# (an" %an&an" !a%at
mr#sa$ '%ar) *r#sa$an '%ar a$ibat tanol !isbab$an $arna Ra!i$al bbas,
Astal!'i! ata# Rasio NAD @ NADH)

,an(a$ %nlitian mn"nai :$ mtanol ma#%#n tanol tr'a!a% or"an
t#b#', a$an tta%i %n#lis bl#m %rna' mnm#$an %nlitian mn"nai :$
mtanol !an tanol tr'a!a% '%ar s+ara mi$ros$o%is %a!a tin"$at '5an +oba)
,rtola$ !ari %rmasala'an trsb#t ma$a %nliti brma$s#! #nt#$ mla$#$an
4
analisis s+ara ilmia' tntan" %n"ar#' %mbrian mtanol !an tanol tr'a!a%
tin"$at $r#sa$an '%ar ti$#s 5istar)
Man:aat !ari %nlitian ini a!ala' #nt#$ mmbri$an in:ormasi ba"i
%nliti lain mn"nai %n"ar#' %mbrian mtanol !an tanol tr'a!a% tin"$at
$r#sa$an sl '%ar %a!a ti$#s Wistar, Mmbri$an in:ormasi $%a!a mas(ara$at
mn"nai %n"ar#' %mbrian mtanol !an tanol tr'a!a% tin"$at $r#sa$an sl
'%ar %a!a ti$#s Wistar, !an !a%at !i"#na$an sba"ai !ata !asar #nt#$ %nlitian
slan&#tn(a !alam lin"$#% %n(ala'"#naan mtanol !an tanol
-nlitian %n#lis brb!a !n"an %nlitian sbl#mn(a) -rb!aan
trlta$ !ari s"i sam%l, >ariabl bbas, !an >ariabl tr"ant#n") ,l#m tr!a%at
%nlitian mn"nai %n"ar#' %mbrian mtanol !an tanol tr'a!a% ti$#s
5istar)
METODE PENELITIAN
-nlitian ini !ila$#$an !i Laboratori#m Histolo"i ;ni>rsitas Di%on"oro,
Laboratori#m -atolo"i Anatomi ;ni>rsitas Di%on"oro, !an Laboratori#m
,iolo"i ;ni>rsitas N"ri Smaran") Wa$t# %la$sanaan %nlitian antara b#lan
A%ril 'in""a <#li 2.11) -nlitian ini a!ala' $s%rimntal laboratori$ !n"an
ran+an"an post test only control group design (an" mn""#na$an binatan" +oba
sba"ai ob&$ %nlitian) -o%#lasi sam%l (an" !itliti a!ala' ti$#s Wistar &antan
#sia 26/ b#lan 1=.62=. "ram (an" s'at, ti!a$ +a+at s+ara anatomis, !an ti!a$
mati ata# sa$it slama %nlitian) -nta%an bsarann(a sam%l br!asar$an
$ritria WHO (ait# &#mla' !alam sat# $lom%o$ minimal lima) -n"ambilan
sam%l !ila$#$an !n"an +ara allocation random sampling) Sam%l (an" tr%ili'
!iba"i mn&a!i m%at $lom%o$ (ait# sat# $lom%o$ $ontrol !an ti"a $lom%o$
%rla$#an !n"an &#mla' ti$#s 0 $or tia% $lom%o$)
Dosis Etanol (an" !ibri$an a!ala' 2 "r1*"1'ari %r oral !i+am%#r !n"an
air 'in""a $a!ar 0.A %a!a $lom%o$ %mbrian tanol 7-18) Dosis mtanol (an"
!ibri$an a!ala' 0 ml1$"1'ari %r oral !ib#at !n"an $a!ar =.A !n"an
mn+am%#r$an air !n"an mtanol !n"an %rban!in"an 1@1 %a!a $lom%o$
5
%mbrian mtanol 7-28) S!an"$an #nt#$ $lom%o$ %mbrian mtanol
brsama tanol 7-/8, tanol ss#ai !n"an !osis ti$#s !i+am%#r$an !n"an
mtanol ss#ai !n"an !osis !iatas $m#!ian !i+am%#r$an !n"an air a"ar
mmili$i >ol#m (an" sama !n"an %rla$#an lain #nt#$ mn+"a' %rb!aan
%n(ra%an a$ibat %rb!aan >ol#m srta mn+"a' tr&a!in(a "an""#an
%n(ra%an mtanol a$ibat $a!ar mtanol (an" tin""i) -mbrian %rla$#an
!ila$#$an slama 13 'ari)
Ti$#s Wistar (an" !i"#na$an !i a!a%tasi trlbi' !#l#) Ti$#s !ibri %a$an
stan!ar !an min#m ad libitum slama %nlitian brlan"s#n") Stla' 'ari $ 1=
ti$#s $m#!ian !i!$a%itasi !an !iambil '%arn(a #nt#$ !ib#at %r%arat !i
Laboratori#m Histolo"i F* ;n!i%)
Tin"$at $r#sa$an (an" !ima$s#!$an a!ala' "ambaran 'isto%atolo"i '%ar
ti$#s 5istar (an" !i%#las !n"an Hmato$silin Eosin lal# !iamati !iba5a'
mi$ros$o%) Stia% Ti$#s Wistar !ib#at sat# %r%arat &arin"an '%ar !an tia%
%r%arat !iba+a !alam lima la%an"an %an!an" (ait# %a!a $m%at s#!#t !an
ba"ian tn"a' %r%arat !n"an %mbsaran 3..B) -mba+aan %r%arat !ari lima
la%an"an %an!an" !i+ari rrata s$or #nt#$ %nilaian sat# ti$#s !n"an sistm s$or
br!asar$an mo!i:i$asi Man3a 4oenigk.
Tabl 1) *ritria %nilaian !ra&at 'isto%atolo"i sl '%ar
Tin"$at *r#sa$an S$or
Normal 1
D"nrasi %arn$imatosa 2
D"nrasi 'i!ro%i$ /
N$rosis 3
Data (an" !i%rol' a$an !i#&i normalitas !n"an u3i saphiro-wilk !an
>ariasi !atan(a !n"an u3i !est of Homogeneity of 5ariances . *m#!ian #nt#$
mn"ta'#i %rb!aan antar $lom%o$ !i"#na$an #&i %aramtri$ Annova
6
!ilan&#t$an #&i Post Hoc !n"an $tnt#an &i$a % C.,.= ma$a tr!a%at %rb!aan
(an" brma$na)
7
Mli'at "ambaran tin"$at $r#sa$an sl '%ar
*ontrol Mtanol
!an
Etanol
Mtanol Etanol
-n"ambilan '%ar
2. E$or Ti$#s
Wistar
A!a%tasi 1 min""#
*
7 = $or 8
-/
7= $or 8
-2
7 = $or 8
-1
7 = $or 8
2 min""#
HASIL
-a!a a5al %nlitian, &#mla' mn+it a!ala' 2= $or) Masin"6masin"
$lom%o$ tr!iri !ari = $or ti$#s !n"an 1 +a!an"an %a!a tia% $lom%o$) Slama
brlan"s#n"n(a %nlitian, %a!a $lom%o$ -1 7tanol8 -2 7mtanol8 !an -/
7mtanol !an tanol8 %a!a min""# $!#a tr!a%at 1 $or ti$#s (an" mati, ti$#s
(an" mati ti!a$ !i"anti !n"an sam%l +a!an"an $arna masi' mmn#'i s(arat
(an" !itta%$an) D$a%itasi mn+it !an %n"ambilan or"an '%ar !ila$#$an %a!a
min""# $!#a)
Tabl 2) Nilai mean !an stan!ar !>iasi
*lom%o$ N Man Stan!ar !>iasi
* = 1,02 .,222
-1 = 2,=2 .,3/22
-2 = 2,32 .,313?
-/ = /,.2 .,/0//
Distrib#si !ata !i#&i mn""#na$an #&i Sa%'iro6Wil$ !an !i!a%at$an
!istrib#si !ata normal 7%D.,.=8 %a!a sm#a $lom%o$ %r+obaan)
Varians !ata !i#&i !n"an mn""#na$an tst o: 'omo"nit( o: Varian+s
!an !i!a%at$an >arians !ata sama !n"an %4.,190 7%D.,.=8) Data %nlitian
$m#!ian !i#&i s+ara analisis !n"an #&i on 5a( Ano>a $arna !i!a%at$an
!istrib#si !ata normal !an >arians !ata (an" sama) -a!a #&i Ano>a !i%rol' nilai
%4.,... 7%C.,.=8 (an" artin(a tr!a%at %rb!aan (an" brma$na %a!a %alin"
ti!a$ 2 $lom%o$)
Analisis !ata !itr#s$an mn""#na$an #&i -ost Ho+ #nt#$ mnilai masin"6
masin" $lom%o$)
8
Tabl /)Hasil #&i statisti$ %rban!in"an antar $lom%o$
EHasil #&i Post-Hoc brma$na, %C.,.=
Dari #&i -ost Ho+, !i%rol' 'asil ba'5a tr!a%at %rb!aan (an"
brma$na antara $lom%o$ * !n"an -1 7.,..28, * !n"an -2 7.,..38, * !n"an
-/ 7.,...8, -1 !n"an -/ 7.,.298, -2 !n"an -/ 7.,.218)
Gambaran 'isto%atolo"i sl '%ar normal, !"nrasi %arn$imatosa,
!"nrasi 'i!ro%i$ !an n$rosis)
Gambaran 'isto%atolo"i '%ar ti$#s Wistar 73..B8) *ontrol 7*8@ sl '%ar normal)
-rla$#an 1 7-18@ !"nrasi %arn$imatosa) -rla$#an 2 7-28@ !"nrasi
'i!ro%i$) -rla$#an / 7-/8@ n$rosis, inti sl %i$noti$)
9
*lom%o$ * -1 -2 -/
* 6 .,..2E .,..3E .,...E
-1 .,..2E 6 .,200 .,.29E
-2 .,..3E .,200 6 .).21E
-/ .,...E .,.29E .,.21E 6
K P
1
P
2
P
3
Pembahasan
Etanol ata# !isb#t &#"a sba"ai til al$o'ol mr#%a$an sn(a5a $imia
(an" %n""#naann(a srin" !i"#na$an %a!a $'i!#%an s'ari6'ari, sba"ai ba'an
:arma$o$inti$ ma#%#n sba"ai ba'an min#man (an" !i$nal !n"an min#man
bral$o'ol) Nam#n !mi$ian %n""#naan tanol (an" brlbi'an ata# !alam
&an"$a 5a$t# (an" %an&an" !a%at mn(bab$an $r#sa$an %a!a '%ar, $arna
mtabolism tanol sba"ian bsar tr&a!i !i!alam '%ar) *r#sa$an a$ibat tanol
!a%at mn(bab$an !"nrasi %a!a sl '%ar, statosis sam%ai n$rosis) Etanol
m#rni !an min#man brla$o'ol !ibban$an %a&a$ (an" brat !alam %n&#alann(a)
;nt#$ mm%rin"an bban %a&a$ !alam %n""#naann(a $ban(a$an %n&#al
ml%as$an %a&a$ trsb#t !n"an mnamba'$an Fat (an" ti!a$ bol'
!i$ons#msi, s%rti mn+am%#r tanol !an mtanol) -ro!#$ s%rti ini !isb#t
al$o'ol !nat#rasi)
=
Mtanol mr#%a$an bnt#$ al$o'ol %alin" s!r'ana (an" m#!a'
mn"#a%,trba$ar, !an bra+#n s'in""a %n""#naann(a ti!a$ !i%r#nt#$an
#nt#$ !i $ons#msi sba"ai ba'an min#man) Etanol !an mtanol $!#an(a
sba"ian bsar !i mtabolism !i '%ar ol' sbab it# %nlitian $ ini !ila$#$an
#nt#$ mli'at tin"$at $r#sa$an sl '%ar &i$a %mbrian mtanol !an tanol
!ibri$ian s+ara brsamaan)
<nis %nlitian (an" tla' !ila$#$an a!ala' %nlitian $s%rimntal
laboratori$ !n"an ran+an"an post test only control group design.
/2
Mn""#na$an
m%at $lom%o$, (ait# ti"a $lom%o$ $s%rimntal !an sat# $lom%o$ $ontrol)
-nlitian !ila$#$an slama 13 'ari !ilan&#t$an !n"an %n"ambilan or"an !an
%mba+aan %r%arat)
Etanol !a%at mnimb#l$an $r#sa$an %a!a '%ar !isbab$an $arna
ra!i$al bbas, astal!'i! !an rasio NAD @ NADH) Mtabolism tanol !i !alam
sl '%ar mn(bab$an %nin"$atan %ro!#$si ra!i$al bbas !n"an brba"ai
m$anism s'in""a tr&a!i strs o$si!ati: (an" a$an mr#sa$ &arin"an 'ati)
12
Mtabolism al$o'ol mm%n"ar#'i rasio NADH @ NAD, NADH (an" tin""i
mn(bab$an %nin"$atan %ro!#$si asam lma$ %a!a sl '%ar)
/.
Sba"ian bsar
10
$r#sa$an &arin"an sl '%ar al$o'oli$ $roni$ !ia$ibat$an ol' astal!'i! (an"
trtimb#n !i !alam 'ati !an !ibbas$an $ !alam !ara' stla' ssoran" min#m
al$o'ol !alam &#mla' bsar) Astal!'i! san"at ra$ti: !an bri$atan s+ara
$o>aln !n"an "#"#s amino, n#$loti!a !an :os:oli%i! #nt#$ mmbnt#$ adduct)
Sala' sat# a$ibat !ari %mbnt#$an adduct6 astal!'i! a!ala' mn#r#nn(a
%mbnt#$an %rotin (an" mmbnt#$ %arti$l li%o%rotin 'ati !an br$#ran"n(a
s$rsi %rotin (an" !%n!nt6t#b#lin) Sba"ai a$ibat "an""#an m$anism
s$rtori$ tr&a!i %nimb#nan tri"lisrol !an %rotin !i !alam 'ati) -nimb#nan
%rotin mn(bab$an in:l#$s air $ !alam '%atosit !an %mbn"$a$an 'ati i$#t
srta mnimb#l$an 'i%rtnsi %orta !an $r#sa$an arsit$t#r 'ati)
/1
Mtanol !a%at mnimb#l$an $r#sa$an %a!a sl '%ar !isbab$an $arna
Ra!i$al bbas, Formal!'i! !an Asam :ormat) Formal!'i! mnin"$at$an li%i!
%ro$si!as (an" !a%at mn"a$ibat$an $r#sa$an sl mmbran !an $matian
sl)
2
Asam :ormat mn"'ambat a$ti:itas o$si!asi mito$on!rial sito$rom,
mn"'alan"i mtabolism o$si!ati: !an mn"a$ibat$an 'i%o$sia &arin"an)
22
-a!a "ambaran 'isto%atolo"i sl '%ar mn#n&#$an a!an(a $r#sa$an
&arin"an a$ibat %mbrian mtanol, tanol !an mtanol tanol) *lom%o$ -/
mmili$i tin"$at $r#sa$an trbrat !n"an rrata s$or /,.2) *lom%o$ *
mmili$i tin"$at $r#sa$an %alin" rin"an !n"an rrata s$or 1,02) *lom%o$ -/
mmili$i tin"$at $r#sa$an trbrat !n"an rrata /,.2, %n(babn(a
$m#n"$inan $arna %ro!#$ mtabolism mtanol !an tanol (an" sama6sama
mmili$i %n"ar#' bsar !alam %ross li%i! %ro$si!asi (an" !a%at mr#sa$ sl
'%ar)Tla' !i!a%at 'asil %nlitian (an" ss#ai !n"an 'i%otsis %n#lis)
Hasil #&i b!a antara $lom%o$ %rla$#an mn#n&#$an %rb!aan (an"
brma$na, (ait# antara $lom%o$ * !n"an -1 7p..,..28 $m#n"$inan
!i$arna$an tanol !a%at mn"a$ibat$an $r#sa$an %a!a '%ar mlal#i
%nin"$atan %ro!#$si ra!i$al bbas, :$ !ari mtabolit astal!'i! !an
%r#ba'an rasio NAD @ NADH, -rb!aan (an" brma$na %a!a $lom%o$ *
!n"an -2 7%4.,..38 !i$arna$an mtanol !a%at mnimb#l$an $r#sa$an %a!a sl
'%ar mlal#i %nin"$atan ra!i$al bbas, :ormal!'i!, !an asam :ormat,
*lom%o$ * !n"an -/7p4.,...8 mn#n&#$an %rb!aan (an" brma$na
11
$m#n"$inan $arna a!an(a :$ to$sisitas !ari $!#a sn(a5a) -1 !n"an
$lom%o$ -/ 7p4.,.298 !an $lom%o$ -2 !n"an -/ 7p..,.218 mn#n&#$an
%rb!aan (an" brma$na $m#n"$inan !i$arna$an :$ to$sisitas (an" lbi'
tin""i %a!a $lom%o$ !n"an !#a sn(a5a 7mtanol tanol8 !iban!in" $lom%o$
!n"an sat# sn(a5a 7matanol ata# tanol sa&a8 )Tr!a%at %r#ba'an "ambaran
'isto%atolo"is (an" ti!a$ brma$na antara $lom%o$ -1 !n"an -2 7p4.,2008)
-rb!aan (an" ti!a$ brma$na %a!a $lom%o$ -1 !n"an -2 $m#n"$inan
$arna $!#a sn(a5a trsb#t mmili$i :$ to$sisitas (an" 'am%ir sama %a!a
$r#sa$an sl '%ar, tta%i man %a!a $lom%o$ tanol 72,=28 lbi' bsar
!iban!in"$an mtanol 72,328
Slama brlan"s#n"n(a %nlitian, tr!a%at ti"a $or ti$#s (an" mati %a!a
masin"6masin" $lom%o$ %rla$#an, %a!a $lom%o$ -1, -2, !an -/ %a!a min""#
$!#a, ti$#s (an" mati ti!a$ !i"anti !n"an sam%l +a!an"an $arna masi'
mmn#'i s(arat (an" !itnt#$an)
Mtanol !an tanol sama6sama mmili$i :$ mr#sa$ sl '%ar) Nam#n,
bila !ibri$an brsamaan, mtanol !an tanol mmili$i :$ sinr"isti$, !imana
$!#a sn(a5a mnin"$at$an $r#sa$an sl '%ar s+ara brma$na bila
!iban!in"$an !n"an $lom%o$ %rla$#an lain3 -mbrian mt'anol !n"an !osis
0ml1$" brat ba!an !a%at mnin"$at$an $r#sa$an sl '%ar s+ara brma$na
!iban!in"$an $lom%o$ $ontrol)-mbrian tanol !n"an !osis 2"r1$" brat
ba!an !a%at mnin"$at$an $r#sa$an sl '%ar s+ara brma$na bila
!iban!in"$an !n"an $lom%o$ $ontrol) Nam#n, %rban!in"an tin"$at $r#sa$an
antara $lom%o$ tanol !an mt'anol ti!a$ mmili$i %rb!aan $r#sa$an (an"
brma$na) Rrata s$or $r#sa$an %a!a sl '%ar (an" %alin" bsar tr!a%at %a!a
$lom%o$ !n"an %mbrian mtanol tanol)
-rl# a!an(a %nlitian lan&#tan !n"an !#rasi %nlitian (an" lbi' lama
!an !n"an !osis mtanol !an tanol (an" lbi' br>ariasi) *arna !osis mtanol
!an tanol sba"ai ba'an min#man $ras san"at br>ariasi !i mas(ara$at)
-nlitian (an" lbi' lama !ila$#$an #nt#$ mn!a%at$an :$ to$sisitas $roni$
(an" lbi' lama)
12
U4APAN TERIMAKASIH
-nliti mn"#+a%$an s(#$#r !an trima $asi' (an" sbsar6bsarn(a
$%a!a !r)Santosa S%)F, !r)*asno S%)-A7*8, !r) E$oG#nianto, MH*s, s"na%
sta: ba"ian *!o$tran Fornsi$ Fa$#ltas *!o$tran ;n!i%, s"na% sta:
laboratori#m Histolo"i !an -atolo"i Anatomi Fa$#ltas *!o$tran ;n!i%, !an
s"na% sta: laboratori#m ,iolo"i ;ni>rsitas N"ri Smaran")
DA/TAR PUSTAKA
1. Nla(an An""a% Miras <am# -"al Lin#) IGN sa5abi Hsrial onlin I)2..2
H+it! 2.11 <an 1.I O$tobr ?) A>ailabl :rom
'tt%@11nasional)$om%as)+om1ra!12..211.1.?12.=02231nla(an)an""a%)miras)&
am#)%"al)lin#
2. O%losan Ma#t *orban T5as ,rtamba' Hsrial onlin I)2.1. H+it! 2.11 <an
13I Mi 12) A>ailabl :rom @htt!255ha"ian&)1)sema"3$)m5be"ita5)!1)san'
maut'k)"ban'te6as'be"tambah'(78((3htm1
3. -sta Miras, ,o+a' 12 Ta'#n I$#t T5as) ,nn( N <o5ono Hsrial onlinI)
2.11 H+it! 2.11 Fb 1/IFbr#ari 2) A>ailabl
:rom
@'tt%@11r"ional)$om%as)+om1ra!12.111.21.21191129=21-sta)Miras),o+a')12
)Ta'#n)I$#t)T5as
4. F6-DI- D-RD ,ar#t -ri'atin Soal T5asn(a S&#mla' -m#!a Hsrial
onlinI) 2.11 H+it! 2.11 :b 1/I Fbr#ari =) A>ailabl
:rom @'tt%@11555)'arian%lita)+om1ra!11==?/1111antar6!ara'1:J%!i%6!%r!6
bar#t66%ri'atin6soal6t5asn(a6s&#mla'6%m#!a1
5. Et'anol) Wi$i%!ia H'om%a" on t' intrntI)2.11 H#%!at! 2.11Fb 22K
+it! 2.11 Fb 2/I A>ailabl
:rom @'tt%@11n)5i$i%!ia)or"15i$i1Et'anolL+itJnot6m(rs61
13
6. Sa:t( !ata :or t'(l al+o'ol, absol#t 72.. %roo:8) HHom%a" on Intrnt I)
2..2 H#%!at! 2..2 Ma( 9 K +it! 2.11 Fb /I A>ailabl
:rom @'tt%@11ms!s)+'m)oB)a+)#$1ET1t'(lJal+o'ol)'tml
7. Mt'anol, A#t'or@ *al(ani *orabat'ina, MD, Cons#ltin" -'(si+ian, Nort'
Co#nt( N#rolo"( Asso+iats, In+, O+ansi!, Cali:ornia)HHom%a" on
IntrntI) 2..9 H;%!at!2..9 No> 9 K +it! 2.11 Fb 2I A>ailabl
:rom @'tt%@11m!i+in)m!s+a%)+om1arti+l111?329.6o>r>i5
2) Mt'anol6In!#+! OBi!ati> Strss in Rat L(m%'oi! Or"ans)
Nara(ana%r#mal <-, Ramas#n!aram S*, S#n!arama'alin"am M,
Rat'inasam( SD) <o#rnal o: O+#%ational Halt') 2..0) 32@2.62?)
93 *ra+#nan Min#man O%losan,Sat# T5as)As% Di!i) Hsrial onlinI) 2.11
H+it! 2.11 :b 1/I /eb"ua"i -3 A:ai1ab1e
;")m
2htt!255buse"31i!utan83$)m5be"ita5*+((+*5-(<<--5Ke"a$unan=Minuman=O
!1)san=Satu=Te6as
10. S'at, T$nisi S#$'oi Min#m S%irit#sH srial onlin I) 2.1. H +it! 2.11 Fb /
I S%t 12) A>ailabl :rom @'tt%@11r"ional)$om%as)+om1ra!12.1.1.91121
..=0=2331S'at))T$nisi)S#$'oi)Min#m)S%irit#s,
11. Al+o'ol IntoBi+ation H 'om%a" in Intrnt I) A>ailabl :rom @
'tt%@11555)m!i+in'alt')+om1al+o'olJintoBi+ation1%a"2Jm)'tm
12. Wn!( Ra%%aFFoAsso+iat -ro:ssor o: ,iolo"(Har:or! Comm#nit( Coll")
)HHom%a" on IntrntI) 2..2 H+it! 2.11 <an 10I A>ailabl :rom@
'tt%@11555)'ar:or!)!#1:a+#lt(15ra%%aFFo1,IO2.31l+t#r1bio2.3l+t#rmat
rials)'tm
(-3 Al+o'oli+ Li>r Disas 6 -at'o%'(siolo"i+al As%+ts an! Ris$ Fa+tors@
-at'o"nsis o: Al+o'oli+ Li>r Disas@ t' M+'anisms o: Li>r Dama"
>Hom%a" on IntrntI) 2..0 H+it! 2.11 <an 10I A>ailabl
:rom@htt!2556663me%s$a!e3$)m5:ie6a"ti$1e57?7((9=?
13) ,#!i(anto A,!$$)Ilm# *!o$tran Fornsi$)Ct)2)<a$arta@,a"ian *!o$tran
Fornsi$ Fa$#ltas *!o$tran ;ni>rsitas In!onsia,199?)Hlm@?1620)
14
1=) Nimant'a Mar$ Wil:r! ! Al5is, C'risto%'r -a#l Da() Gnti+s o:
Al+o'oli+ Li>r) Disas an! Nonal+o'oli+ Fatt( Li>r Disas) 2..? H +it!
2.11 Fb 1? I) A>ailabl :rom @ 'tt%s@11555)t'im6
+onn+t)+om1&o#rnals1abstra+t1sl!1!oi11.)1.==1s62..0690.1?.
16. Di$tat Anatomi Ab!omn@,a"ian Anatomi Fa$#ltas *!o$tran ;ni>rsitas
Di%on"oro) 2..2
1?) So&oto,Sot!&o,S#ltana M,!$$)L+t#r Not Histolo"i II)Smaran"@,a"ian
Histolo"i Fa$#ltas *!o$tran ;ni>rsitas Di%on"oro,2.1.@/?63.
18. Hi!a(at,Hrna5ati,Gambaran To$si$ Etanol %a!a Sl Hati. H+it! 2.11 <an =I
A>ailabl :rom@'tt%@11:il)#%i)!#1Dir$tori1)%!:
19. Mt'anol) Wi$i%!ia H'om%a" on t' intrntI)2.11 H#%!at 2.11 <an 20K
+it! 2.11 Fb 2/I A>ailabl From @ 'tt%@11i!)5i$i%!ia)or"15i$i1Mtanol
2.) Gao:n" W#, <ian+'n" Gan", C'an"mian S#n, Min'on" L#an, <iao S'i,
<ianmin H#) E::+t o: Ta#rin on Al+o'oli+ Li>r Disas in Rats)Amino
A+i!s) 2..9 H+it! 2.11 Fb 19I)/0@3=?N303
21. Lo < S+'%, Robin < Sla#"'tr, < Allistr Val, D Mi+'al G ,asl(, A
Saman Wit' ,lin!nss an! Con:#sion) ,ritis' M!i+al <o#rnal) 2..9 H +it!
2.11 Fb 11 I) A>ailabl :rom @ 'tt%@11555)bm&)+om1+ontnt1//91
bm&)b/929):#ll
22. Mt'anol) H 'om%a" on Intrnt I) 2..? H #%!at 2.1.K +it! 2.11 Fb 1= I)
A>ailabl :rom @ 'tt%@11+#rri+#l#m)toBi+olo"()5i$is%a+s)nt1
2)2)=)2)=OMt'anol
23. Vina( *, Ab#l *, Nlson Fa#sto, Ri+'ar! NM) Robbins ,asi+ -at'olo"() 2
t'
!) -'ila!l%'ia@ Sa#n!rs Els>irK 2..?)
24. Wan&#n G#o, Pian" Wan", Gon""a LanFi, O#F'# L#ob#, Miao'on" Ma,
Gin"+'n" Wan"t all) 2.1. H +it! 2.11 Fb 12 I) A>ailabl :rom @
'tt%@11onlinlibrar()5il()+om1!oi11.)1..21a&m")b)/1.2.1abstra+t
2=) *asno,-rast(o A )-atolo"i 'ati !an sal#ran m%!# $stra
'%ati$)Smaran"@,a!an -nrbit ;ni>rsitas Di%on"oro,2..2) 1?621
20) S$r(!l5s$a ElFbita) ToBi+olo"i an! Mtaboli+ ConsQ#n+s o: Mt'anol
-oisonin")2../)11@2??629/
15
27. A) GramnFi, F) Ca%#to, M) ,islli, F) *#ria, E) Lo""i, -) An!ron, M)
,rnar!i)2..0H+it! 2.11 Fb 1? I) A>ailabl :rom @
'tt%@11555)m!s+a%)+om1>i5arti+l1=3=119J2
22) Man&a Roni"$) Histolo"i+al -attrns in Dr#" In!#+! Li>r Dissas)<o#rnal
o: Clini+al -at'olo"i Hsrial onlinI)2..9 H+it! 2.11 Fb 1?IK 02@3216392)
29) -ram#!(a -, Fa!illa) -n"ar#' Lama -mbrian Mtanol =. A %r oral
Tr'a!a% Tin"$at *r#sa$an Sl Gastr Ti$#s Wistar) 2.1.)
30. Al+o'oli+ li>r !isas) Wi$i%!ia H'om%a" on t' intrntI)2.11 H#%!at
2.11 <an 20K +it! 2.11 <#li 29I A>ailabl From @
'tt%@11n)5i$i%!ia)or"15i$i1Al+o'oli+Jli>rJ!isas
31. Mar$s,Da5n ,, Mar$s,Allan D, Smit',Colln M) ,io$imia *!o$tran Dasar)
<a$arta@ EGC, 2...) Hal @ =?0
32. Sastroamoro S, Ismail S) Dasar6!asar mto!olo"i %nlitian $linis) 2
n!
!)
<a$arta @ Sa"#n" StoK 2..2)
16