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1. Describe the principles, advantages, and limitations of the light microscope, transmission electron microscope,
and scanning electron microscope.
• Electron microscopes reveal organelles that are impossible to resolve with the light microscope. However, electron
microscopes can only be used on dead cells.
• Light microscopes do not have as high a resolution, but they can be used to study live cells.
• Transmission electron microscopes (TEMs) are used mainly to study the internal ultrastructure of cells. A TEM aims an
electron beam through a thin section of the specimen.
• Scanning electron microscopes (SEMs) are useful for studying surface structures. The SEM has great depth of field,
resulting in an image that seems three-dimensional.
1. Describe the major steps of cell fractionation and explain why it is a useful technique.
• In a prokaryotic cell, the DNA is concentrated in the nucleoid without a membrane separating it from the rest of the cell.
• In eukaryote cells, the chromosomes are contained within a membranous nuclear envelope.
1. Explain why there are both upper and lower limits to cell size.
The Cytoskeleton
20. Describe the functions of the cytoskeleton.
21. Compare the structure, monomers, and functions of microtubules, microfilaments, and intermediate filaments.
22. Explain how the ultrastructure of cilia and flagella relates to their functions.