Scribd Logo
Fazer o upload

Bem-vindo(a) ao Scribd!

  • Triglycerides

    Enviado por

    Yasir Mushtaq
    13 visualizações2 páginas
    This document describes a method for determining triglyceride levels. It involves an enzymatic hydrolysis of triglycerides using lipases to produce glycerol and fatty acids. The glycerol is then converted by a series of enzymatic reactions to produce hydrogen peroxide, which reacts with 4-aminophenazone and chlorophenol under catalytic influence to form a quinoneimine dye. The absorbance of this dye is then measured and is directly proportional to the triglyceride concentration in the sample. The method uses a reagent containing the necessary enzymes, substrates, and indicators. Samples and standards are incubated and their absorbance read to determine triglyceride content.

    Descrição original:

    a practical about triacylglycerides

    Direitos autorais

    © © All Rights Reserved

    Formatos disponíveis

    DOC, PDF, TXT ou leia online no Scribd

    Você considera este documento útil?

    Este conteúdo é inapropriado?

    Denunciar este documento
    This document describes a method for determining triglyceride levels. It involves an enzymatic hydrolysis of triglycerides using lipases to produce glycerol and fatty acids. The glycerol is then converted by a series of enzymatic reactions to produce hydrogen peroxide, which reacts with 4-aminophenazone and chlorophenol under catalytic influence to form a quinoneimine dye. The absorbance of this dye is then measured and is directly proportional to the triglyceride concentration in the sample. The method uses a reagent containing the necessary enzymes, substrates, and indicators. Samples and standards are incubated and their absorbance read to determine triglyceride content.

    Direitos autorais:

    © All Rights Reserved
    Baixe no formato DOC, PDF, TXT ou leia online no Scribd
    Sinalizar o conteúdo como inadequado
    13 visualizações2 páginas

    Triglycerides

    Enviado por

    Yasir Mushtaq
    This document describes a method for determining triglyceride levels. It involves an enzymatic hydrolysis of triglycerides using lipases to produce glycerol and fatty acids. The glycerol is then converted by a series of enzymatic reactions to produce hydrogen peroxide, which reacts with 4-aminophenazone and chlorophenol under catalytic influence to form a quinoneimine dye. The absorbance of this dye is then measured and is directly proportional to the triglyceride concentration in the sample. The method uses a reagent containing the necessary enzymes, substrates, and indicators. Samples and standards are incubated and their absorbance read to determine triglyceride content.

    Direitos autorais:

    © All Rights Reserved
    Baixe no formato DOC, PDF, TXT ou leia online no Scribd
    Sinalizar o conteúdo como inadequado
    de 2

    OBJECT: TRIGLYCERIDES

    IVd
    Method
    The triglycerides determined ater en!ymatic hydrolysis "ith li#ases$
    Indicator is %&inoneimine ormed rom hydrogen #ero'ide( )*amino*anti#yrine and
    )*chloro#henol &nder the catalytic inl&ence o #ero'ides$
    Reaction principle:
    Triglycerides
    li#ase
    glycerol + atty acids
    Glycerol + ,T-
    G.
    glycerol*/*#hos#hate + ,D-
    Glycerol */*#hos#hate + O0
    G-O
    dihydro'yacetone #hos#hate + 10O0
    10 O0 + )*aminoanti#yrine
    -OD
    %&inoneimine + 1Cl + 10O
    +)*chloro#henol
    Contents
    RGT$ 2$3 ml4 255 ml or 035 ml Monoreagent
    -i#es 6&er 35 mmol7l
    )*chloro#henol 3 mmol7l
    )*amino#hena!one 5$03 mmol7l
    Magnesi&m ions )$3 mmol7l
    ,T- 0 mmol7l
    Li#ase 8 2/55 97l
    -ero'idase 8 355 97l
    Glycerol .inase 8 )55 97l
    Glycerol*/*#hos#hate o'idase 8 2355 97l
    STD$ / ml Standard
    Triglycerides 055 mg7dl
    Or 0$0: mmol7l
    -i#ette into c&;ettes RB Sam#le STD
    Sam#le STD <<$ 25 = l
    RGT = l 2555 = l
    Mi' and inc&6ate or 25 min$ at 0o<03
    o
    C or or 3 min at />
    o
    C$ Meas&re the a6sor6ance o the
    sam#le ?@, sam#leA and the standard ?@ , STA against the reagent 6lan. "ithin B5 min$

    Você também pode gostar