‘Animal Cell Culture Applications: A.Model system: Call culture are used as model system to study basic cell biology and biochemistry, to study the interaction Between col and disease causing agents tke bacteria, vis, to study the effect of drugs, to study the process 2.Cancer Research: ‘he basic difference between normal cell and cancer cell can be studied using animal cell culture technique, as Both cols ean be cultured im laboratory. Normal cells can be converted into cancer ealis by using radkation, Chemicals and viruses. Thus, the machanism and cause of cancer can be studied. Call culture can be used to Getermine the effective crugs for selectively Gestroy ofly cancer cells 3.wwretogy: Animal col cultures are used to replicate the viruses instead of animals for the production of vaccine. call Culture can sleo be used to detect and isolate vinises, and also to study growth and cevelopment cycle of Sindece. Itz also seed to study the mode of infection: 4-Tosiety Testing: ‘Animal call culture is used to study the effects of new drugs, cosmetics and chemicals on survival and growth of Thumber of typer of coll: Expecially war and kicnay cells, Cultura srimal collz ara alco used te determina the ‘raximum permeable dosage ef new drugs. S.vaccine production: example vaccines fer deadly diseases lke polio, rabies, chicken pox, measles and hepatitis B are produced uoing Shima ee cuture, 6 Genetcaly Engineered Protein: anim cell cultures are used to produce commercisly impartant genetically engineered proteins such as monoctonal antibodies, insulin, hormones, and much mere. ‘7Replacement Tissue or gant ‘imal cell cultie can be used as replacement tissue or organs. For example artifical skin can be produced using this technique to teat patients with bums and ulcers. However research is going on atic organ culture such as iver, kidney and pancress. Organ culture techniques and research are being conducted on bath embryonic and adult stam cal culture, These cells have the capacity to LSttertite inte many siferant types of cals and organs, Its belvad that by leaming to contol the development and efferenbaton of these cells may be used to treat vanety of medical conditions. Genetic Counseling: Fotal cel cuture extracted from pregnant women can ba used to study of examine the abnormalities of chromesomes, genes using karyotyping, and those findings can be used in early detaction of ftal disorders. 9.Genetic Encinesing: Cultured animal ces can be used to introduce nev ganstc materia lie DNA or RNA into the cal, These can be used to study the fepression af new genes and ts effact on the health ofthe cell. nsact calls are used to produce commercially mportant proteins by Infectng them with genetically staves bacuovinises 10.Gene Therapy: Cultured enimal cels can be genetcaly altered and can be used in gene therapy technique. Fist cells are removed from the patient lacking a functional gona or missing a functional geno. These gonas are replacad by functional genes and altored cals are culture and {grown in laboratory condition, Then thace altered cells are introduced into the patient. another mathod is by using val vector, unctional gene is inserted into the genome of viral vector and then they ae alowed to infect the patient, in the hope that the mising (gene will be expressed with the halp of the viral vector 1 Drug Screening and Daveloment: anima! cel ulfires are used fo etudy the cytotoxicity of new drug. This alsa used to nd cut the effactive and safe dosage of nee rugs, How these tests are being consucted in 384 and 1536 wal plates, Cel-based assay plays an important olen pharmaceutical