PROJECT RESPIRATION &

DIGESTION
VISUAL ANALYSIS OF RESPIRATORY AND DIGESTIVE TISSUE

Presented By
Christina Watson

Ashley Palafox

Beverly Howard

James Yuanxin Li

Amy Choi

Lucy Renderos

Tayo Oyesiku

Jennifer Heimbach

Sam Cohen

TOOTH

Enamel
Dental Sac

Dental Papilla

By Beverly Howard

Leica 10x DIC

TOOTH

By Beverly Howard

Leica 20x BF

TOOTH

By Beverly Howard

Leica Red 20x

TOOTH

Leica Green 10x

By Beverly Howard

TASTE BUDS

Vertical

Skeletal Muscle

By Beverly Howard

Leica BF 10x

TONGUE BUDS

Leica 10x Dapi

By Beverly Howard

LUNG ELASTIC FIBERS

Respiratory
Bronchiole

By Beverly Howard

Brionchiole
Brionchiole

Leica DIC 10x

RECTUM

By Lucy Renderos

Leica Cy3 20x

RECTUM

By Lucy Renderos

Leica GFP 20x

LUNG AND BRACHI

Bronchiole

Respiratory Bronchiole
By Lucy Renderos

Leica 20x GFP3

LUNG AND BRONCHI

Epithelium
Lamina Propria
Clara Cells

By Lucy Renderos

Leica 20x CY3

ILEUM

Nikon 27ms Exposure for both, 10x (L), 20x (R)

By Sam Cohen

ILEUM

Nikon 20x, 5.03 second exposure

By Sam Cohen

LUNG: ALVEOLUS

Leica DM 500 10x linear 32.6 ms

By Jennifer Heimbach

LUNG: ALVEOLUS

Leica DM 500, 10x linear

Top R: 32.6 ms
Top L: 1.0 s (histogram 0-92)
Bottom: composite overlay

By Jennifer Heimbach

NASAL SEPTUM

Nikon 80I 20x, 23ms Exposure

By Sam Cohen

NASAL SEPTUM

Nikon 20x, 257ms exp, Flour Overlay

By Sam Cohen

RECTUM

Nikon 80I 20x 22ms Exposure

By Sam Cohen

ILEUM

Goblet Cells

Peyers
Patch

Villi

Plicae circulares

By Amy Choi

Leica DM500, 10X

ILEUM

BF

By Amy Choi

Peyers
Patche

Leica DM500, 10X

DUODENUM
Muscosal
layer

By Amy Choi

Muscularis
Externa

Glands of Brunner

Muscularis mucosa

DUODENUM

BF

By Amy Choi

Leica DM500, 40X

JEJUNUM INCREASED NUMBERS OF GOBLET CELLS

By Amy Choi

Submucosa

Muscularis
muscosa

Villi

JEJUNUM

BF

By Amy Choi

Lecia DM500, 10X

TASTE BUDS

Basal cell

Gustatory
Cell
Sustentacular Cell

By Ashley Palafox

Leica DM500 40x, 10.6ms, BF

Taste buds are

located on the
tongues papillae,
and are
responsible for
detecting the five
element of taste
perception: sweet,
salty, bitter, sour,
and umami.

BRONCHIOLE

epithelium

Smooth muscle

By Ashley Palafox

Leica DM500 40x, 7.55ms, BF

The bronchioles
are tubes in the
lungs through
which air passes to
the alveoli.
Bronchioles branch
of the the bronchi,
and are part of the
conducting zone of
the respiratory
system.
They
predominately
consist of smooth
muscle lined with
epithelium.

BRONCHIOLE

By Ashley Palafox

Leica DM500 40x, 776.8ms, R fluorescence

SUBLINGUAL GLAND
The sublingual glands
are major salivary
glands, located
beneath the tongue.
They provide 3-5% of
the total salivary
volume.
Goblet Cell Acini

By Ashley Palafox

Leica DM500 40x, 41.6ms, gamma 1.4, BF

SUBLINGUAL GLAND

Leica DM500
Left: 40x, 704.6ms, G
By Ashley Palafox

Right: 40x, 218.5ms, R

LIVER (CENTRAL VEIN)

DM 500 40x, Brightfield:

Exp 74.3ms, gain- 1.2x, sat- 1.50, gamma- 0.57
By Tayo Oyesiku

LIVER (CENTRAL VEIN)

DM 500 40x, Green:

Brightness 43%, sat- 1.50, gamma- 0.57
By Tayo Oyesiku

LIVER (HEPATOCYTES)

DM 500 40x, Brightfield:

Exp 74.3ms, gain- 1.2x, sat- 1.50, gamma- 0.57
By Tayo Oyesiku

LIVER (HEPATOCYTES)

UV

Leica DM500 40x

By Tayo Oyesiku

UV: Exposure 1.0s, Gain 1.4x

Saturation 1.25, Gamma 0.99
Blue: Brightness 43%, Sat 1.2
Gamma 0.99
Green: Exposure 1.0s, Gain 1.4x
Saturation 1.25, Gamma 0.99

1.
2.

TRACHEA

3.
4.
5.
6.
7.
8.
9.

Leica DM500, Brightfield, 10x

By James Yuanxin Li

Lumen
Pseudostratified
epithelium with cilia
Blood vessels
Laminia propria
(connective tissue)
Submucosa
Smooth muscle
Hyaline cartilage with
chondrocytes
Dense connective
tissue
Adipose tissue

The trachea connects

the pharynx and larynx
to the lungs, allowing
the passage of air. It is
lined with an epithelium
that has goblet cells
that produce mucins
and cilia that help
remove bacteria and
debris.

TRACHEA

Leica DM500 10x

Top (L, R): Brightfield, Green N2.1 filter, Bottom (L, R): Blue i3 filter, Overlay
By James Yuanxin Li

1.
2.
3.
4.

Epithelium
Intestinal glands
Mucosa
Muscularis
mucosa
(smooth
muscle)
5. Submucosa

COLON

The colon (large

intestine) removes
water, salt, and some
nutrients and forms
stool. Muscles line
the colon's walls,
squeezing its
contents along.
Bacteria coat the
colon and its
contents.

By James Yuanxin Li

Leica DM500, Brightfield, 4x

COLON

Leica DM500 4x
Top (L, R): Brightfield, Green N2.1 filter, Bottom (L, R): Blue i3 filter, Overlay
By James Yuanxin Li

ESOPHAGUS
(UPPER CROSS-SECTION)

1. Muscularis
externa
2. Stratified
squamous
epithelium (nonkeratinized)
3. Lamina propria
The upper portion
contains the upper
esophageal sphincter
(UES), which is a bundle
of muscles at the top of
the esophagus. The
muscles of the UES are
under conscious control,
used when breathing,
eating, belching, and
vomiting. They keep food
and secretions from
going down the windpipe.

By James Yuanxin Li

Leica DM500, Brightfield, 4x

ESOPHAGUS
(UPPER CROSS-SECTION)

Leica DM500 4x
Top (L, R): Brightfield, Green N2.1 filter, Bottom (L, R): Blue i3 filter, Overlay
By James Yuanxin Li

ILEOCECAL JUNCTION

DM 500 10x, Brightfield:

Exp 2.72s, gain- 1.2x, sat- 1.55, gamma- 0.99
By Tayo Oyesiku

ILEOCECAL JUNCTION

UV

Leica DM500 10x

By Tayo Oyesiku

UV: Brightness 38%

Saturation 1.25, Gamma 0.99
Green: Brightness 38%, Sat 1.55
Gamma 0.99
Blue: Exposure 739.8ms, Gain 1.2x
Saturation 1.55, Gamma 0.99