PROPOSAL SKRIPSI

PENGARUH LAMA PENYIMPANAN DARAH EDTA SUHU

40C TERHADAP BEBERAPA PARAMETER PEMERIKSAAN
COMPLETE BLOOD COUNT PADA MAHASISWA DIV
TEKNOLOGI LABORATORIUM MEDIK
TINGKAT 1, 2 DAN 3 TAHUN 2015

Ayu Putu Prima Septiani

40.01.11.0003

PROGRAM STUDI
DIV TEKNOLOGI LABORATORIUM MEDIK
STIKES PERDHAKI CHARITAS
PALEMBANG
2015

PERSETUJUAN PROPOSAL
Proposal Skripsi dengan judul :
Pengaruh Kadar Timbal Terhadap Kadar Hematokrit Dalam Darah Juru
Parkir di Pasar Tradisional Kota Palembang
Amelia Nadhila, NIM : 40.01.11.0002, Tahun : 2015
Telah disetujui untuk diuji di hadapan
Tim Penguji Proposal Skripsi
Program Studi DIV Teknologi Laboratorium Medik
STIKes Perdhaki Charitas Palembang
Pada Hari, Kamis Tanggal 4 Februari 2015
Pembimbing I

Pembimbing II

Rosnita Sebayang, SKM., M.Kes

Ian

Kurniawan,

ST.,

M.Eng
YPKK 162.06.2011

YPKK 214.01.2013
Mengetahui,

Ka Prodi D IV Teknologi Laboratorium Medik

Maria Nur Aeni, SKM., M.Kes

YPKK 163.06.2011

PERNYATAAN
Dengan ini menyatakan bahwa dalam proposal ini tidak terdapat karya yang
pernah diajukan untuk memperoleh gelar kesarjanaan di suatu perguruan tinggi,
dan sepanjang pengetahuan saya tidak terdapat karya atau pendapat yang pernah
ditulis atau diterbitkan oleh orang lain, kecuali yang secara tertulis diacu dalam
naskah dan disebutkan dalam daftar pustaka.

Palembang, 4 Februari 2015

Ayu Putu Prima Septiani

40.01.11.0003

DAFTAR ISI
Halaman Judul.......................................................................................................... i
Halaman Persetujuan.............................................................................................. ii
Halaman Pernyataan.............................................................................................. iii
Daftar Isi................................................................................................................ iv
Daftar Tabel........................................................................................................... vii
Daftar Gambar..................................................................................................... viii
Daftar Lampiran..................................................................................................... ix
BAB I Pendahuluan..................................................................................................1
A. Latar Belakang.............................................................................................1
B. Rumusan Masalah........................................................................................5
C. Tujuan Penelitian..........................................................................................5
1. Tujuan Umum........................................................................................5
2. Tujuan Khusus........................................................................................6
D. Manfaat Penelitian.......................................................................................6
1. Bagi Institusi..........................................................................................6
2. Bagi Masyarakat.....................................................................................6
3. Bagi peneliti...........................................................................................6
E. Keaslian Penelitian.......................................................................................7
BAB II Tinjauan Pustaka.......................................................................................10
A. Timbal........................................................................................................10
1. Sifat......................................................................................................10
2. Sumber.................................................................................................11
3. Kegunaan..............................................................................................12
4. Absorpsi...............................................................................................13
5. Distribusi di Tubuh...............................................................................14
6. Ekskresi................................................................................................15
7. Efek Klinis...........................................................................................16
8. Toksisitas Timbal..................................................................................17
9. Batas Pencemaran Timbal....................................................................19
B. Hematokrit..................................................................................................20
1. Sel Darah Merah..................................................................................20
2. Produksi Sel Darah Merah...................................................................21
3. Hematokrit Darah.................................................................................22
C. Pengaruh Timbal Terhadap Hematokrit.....................................................23
D. Spektrofotometer........................................................................................25
1. Spektrofotometer Serapan Atom..........................................................25
2. Spektrofotometer Serapan Atom Graphite Furnace.............................26
4

E. Kerangka Konsep.......................................................................................27
F. Hipotesis.....................................................................................................27
BAB III Metode Penelitian....................................................................................28
A.
B.
C.
D.

E.
F.
G.
H.

I.

J.
K.

Jenis Penelitian...........................................................................................28
Lokasi Penelitian........................................................................................28
Waktu Penelitian........................................................................................28
Subjek Penelitian........................................................................................28
1. Populasi................................................................................................28
2. Sampel..................................................................................................29
3. Perhitungan Besar Sampel...................................................................29
4. Teknik Sampling..................................................................................30
Rancangan Penelitian.................................................................................30
Variabel Penelitian.....................................................................................31
Definisi Operasional...................................................................................31
Alat dan Bahan Penelitian..........................................................................32
1. Alat-Alat...............................................................................................32
2. Bahan....................................................................................................33
3. Reagen..................................................................................................33
Cara Kerja..................................................................................................33
1. Pengambilan Sampel Darah.................................................................33
2. Pemeriksaan Kadar Timbal Darah dengan GF-AAS...........................34
3. Pemeriksaan Kadar Hematokrit dengan Sysmex.................................34
Analisis Data..............................................................................................34
Jadwal Bulanan..........................................................................................35

Daftar Pustaka........................................................................................................36