Screening of Antiinflammatory Drugs

Lab # 4

Outline

Describe the inflammatory response

Anti-inflammatory drugs; MOA, Uses, SE
Explain methods for screening of Antiinflammatory drugs
Discuss the lab work

14-03-07

Gamra Hamad

Inflammation

Response of immune system to foreign organism

or antigenic substance
Characterized by edema, erythema, swelling, and
pain or tenderness

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Gamra Hamad

Inflammatory Mediators
Phospholipids

http://nnsearch.qsrch.com/shp.php
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Phases of inflammation

Acute: vasodilation & increase capillary

permeability

Delayed: infiltration of leukocytes and phagocyte

cells

Chronic proliferative: tissue degeneration and

fibrosis

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Gamra Hamad

Anti-inflammatory Drugs
Steroidal: ex. Corticosteroids
(inh. gene transcription )
Non-steroidal Anti-inflammatory Drugs (NSAID)
(inh. COX)
Examples: ASA, indomethacin, ibuprofen
Selective COX-2 inhibitors

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Gamra Hamad

NSAID

Uses: rheumatoid arthritis

SE: GI adverse effects, renal insufficiency,

bronchospasm

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Gamra Hamad

Screening methods For Antiinflammatory Drugs

Granuloma Pouch edema methods

Croton oil
Control vs. treated
Calculate % inhibition

Adjuvant-induced arthritis

ID Tubercle bacilli
Control vs. treated
Calculate % change in edema & body wt. over 14
days

14-03-07

Gamra Hamad

Screening methods For Antiinflammatory Drugs

Rat-paw edema

Animal 6 rats (3 control, 3 treated)

Drugs: carragenin (2%, 0.1 ml SC.), indomethacin 2
mg/kg IP

14-03-07

Gamra Hamad

Cont.
Control group

Treated group

Rt.

Lt.

Rt.

Lt.

2.9

1.5

1.9

1.3

3.1

1.3

1.7

1.4

1.6

1.6

Mean

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Calculation

% of edema produced by carragenin alone

= A B X 100 = X %
B
% of edema produced by carragenin after ttt with indomethacin
= C D X 100 = Y %
D
% reduction of edema by indomethcin
= X Y=
%

14-03-07

Gamra Hamad