CellularRespirationandPhotosynthesisLab

Introduction:
Plantscarryoutbothphotosynthesisandrespirationatthesametime.During
photosynthesis
,
plantsareusingtheenergyofthesuntomakefood(
glucose
).Thechemicalreactionlookslikethis:

chlorophyll
6CO
+6H
0
2
2

C
H
O
+6O
6
12
6
2

light

Remember,photosynthesiscanonlyhappenwhenplantshavelight!

During
cellularrespiration
,plantsareusingthisfoodenergy(glucose),tomakeenergy(
ATP
).
Chemically,therespiratoryprocesslookslikethis:

C
H
O
+6O

6
12
6
2

6CO
+6H
0+energy!
2
2

Rememberthatplantscarryoutrespirationallthetime!

WhereistheCO
?
2
Theenergyfromcellularrespirationisusedtomakemolecules,movematerialsaroundwithinthe
plant,grow(createnewcells),andreproduce.Noticethatinphotosynthesis,CO
(carbondioxide)is
2
a
reactant
.ThismeanstheCO
isbeingusedupasitisfixed(changed)intoglucosemolecules.
2
DuringcellularrespirationtheoppositeistruetheCO
isa
product
.Theplantpushesoutthe
2
energyinglucosebybreakingitdown.TheCO
isgivenoffintothewateroratmosphere.
2

Therelationshipbetweenthesetwoprocessesisspecial.Itallowsplantstorecycle(reuse)someof
thethingstheplantmakestheCO
isgivenoffduringcellularrespirationcanbereusedduring
2
photosynthesis.

Whatisbromothymolblue?
Inthislab,youwilltrytodemonstratethechangeincarbondioxidewhenthecommonfreshwater
plantElodeaisplacedunderdifferentconditions.Youwillbeusing
bromothymolblue
,asawayto
determinethepresenceorabsenceofCO
.
2

Bromothymoliscalledan
indicator
,becauseitindicates(shows)whenCO
ispresentbychanging
2
color.Itchangescolorduetoachangein
pH
.WhenCO
isinwater,itforms
carbonicacid
.This
2
lowersthepHofthewaterandcausesthebromothymolbluetochangeitsappearance.

The
bromothymolbluechangesfrombluetoyellowwhenCO
ispresent.IftheCO
inthewatergoes
2
2
away,thebromothymolbluewillchangebacktoblue.


CellularRespirationandPhotosynthesisLab

Introduction:
Plantscancarryoutbothphotosynthesisandrespirationsimultaneously.Duringphotosynthesis,
plantsareusingtheenergyofthesuntobuildmoleculeswhicheffectivelystorethisenergy(glucose).
Chemically,thephotosyntheticreactionlookslikethis:
chlorophyll
6CO
+6H
0
2
2

C
H
O
+6O
6
12
6
2
light

Remember,photosynthesiscanonlyhappenwhenplantshavelight!

Duringrespiration,plantsareusingthisstoredenergy(glucose),tofueltheirmetabolicprocesses.
Chemically,therespiratoryprocesslookslikethis:

C
H
O
+6O

6
12
6
2

6CO
+6H
0+energy!
2
2

Rememberthatplantscarryoutrespirationallthetime!

WhereistheCO
?
2
Amongotherthings,theconvertedenergyfromrespirationisusedtomakemolecules,move
materialsaroundwithintheorganism,grow(createnewcells)andreproduce.Noticethatin
photosynthesis,CO
(carbondioxide)isbeingusedupasitisfixedintoglucosemolecules.During
2
respirationtheoppositeistrue.Astheplantreleasestheenergystoredinglucosebybreakingit
down,CO
isbeinggivenoffintothesurroundingwateroratmosphere.Therelationshipbetween
2
thesetwoprocessesisspecialinthatitallowsplantstorecyclesomeoftheirproducts.(WhileCO
is
2
beinggivenoffduringrespiration,itcanbereusedduringphotosynthesis.)

Whatisbromothymolblue?
Inthislab,youwilltrytodemonstratethechangeincarbondioxidewhenthecommonfreshwater
plantElodeaisplacedunderdifferentconditions.YouwillbeusingapHindicator,bromothymolblue,
asameansofdeterminingthepresenceorabsenceofCO
.
2

Bromothymoliscalledanindicator,becauseitchangescolorwhenCO
ispresent.Itchangescolor
2
duetoachangeinpH.WhenCO
isdissolvedinwater,itformscarbonicacid.ThislowersthepHof
2
thesolutionandcausesthebromothymolbluetochangeitsappearance.IftheCO
inthewatergoes
2
away,thebromothymolbluewillchangebacktoblueduetothepHchange.

PhotosynthesisandRespirationLabSheet Name:__________________________

Question:
Whatistheeffectoflight/darkontheproduction(making)ofCO
inElodea?
2

IndividualReading
1.Howdoplantsuse
photosynthesis?

Inphotosynthesis,isCO
2
usedorproduced
(made)?

2.Howdoplantsuse
cellularrespiration?

Incellularrespiration,is
CO
usedorproduced
2
(made)?

3.Whatisbromothymol
blue?Howisitused?

BromothymolBlueObservations

4.Whathappenedwhen
youbreathedintothe
bromthymolblue
solution?Whydidthis
happen?

ExperimentalDesign
5.Lookatthe
experimentalsetup
picturedbelow.

6.Answerthequestions
inthespacebelowyour
drawing.

Question:
Whatistheeffectoflight/darkontheproduction(making)
ofCO
inElodea?
2

Data:

Whatisyourindependentvariable?(whatchanges/thecause)

Whatisdependentvariable?(theeffect)

Whatareyourconstants?(thingsthatstaythesame)

Whatisyourcontrol?(setupthatisusedforcomparison)

Predictwhatcoloreachtubewillbeattheendoftheexperiment.Draw
yourpredictionsontothediagram.

Samples

Initial(Starting)Color

Final(Ending)Color

ControlDark

ControlLight

ElodeaDark

ElodeaLight


Question:
Whatistheeffectoflight/darkontheproduction(making)ofCO
inElodea?
2

Materials:
Elodeaplants
Aluminumfoil
4Largecleantesttubes
Bromthymolbluesolution
Tapeandmarkingpenorwaxpencil1StrawsLarge
Plasticwrapforsealingtesttubes
1250mLFlask
100mLgraduatedcylinderSafetygoggles
Lamp
Testtuberack

Part1:BromothymolBlueSolution
1.RememberthatyouarealivinggeneratorofCO
!Putonyoursafetygogglesforthispart.
2
2.Useastrawtogentlyblowintoa250mlflaskcontainingbromothymolblue.
3.Continueblowinguntilthebromthymolbluechangescolors.Stopblowingwhenyouobservea
colorchange.

CAUTION:
Becarefulnottoswallowanybromothymolorsplashitinyourface.Itistoxicifswallowed!

OnyourLabReportSheet
underBromothymolBlueObservations,recordyouranswers.
Whathappenedwhenyouexhaledintothebromothymolbluesolution?Why?

OnyourLabReportSheet
,asagroupbrainstormhowyoucoulddesignanexperimentto
answerthefollowingquestion:

Whatistheeffectoflight/darkontheproduction(making)ofCO
inElodea?
2

GROUPYPROCEDURES(StartingwithYellow):

Question:
Whatistheeffectof
light/dark
ontheproduction(making)ofCO
inElodea?
2

Materials:
Elodeaplants
Marker
2plasticcontainers 1graduatedcylinderwith
30mL
ofbromothymolbluesolution
1Straw
(
thesolutionisatthematerialsstationpour30mLintoyourgrad.cylinder
)
Safetygoggles

Part1:PreparingtheBromothymolBlueSolution
1.RememberthatyouarealivinggeneratorofCO
!Putonyoursafetygogglesforthispart.
2

2.Yourgraduatedcylindershouldalreadyhave30mLofbromothymolbluesolution.

3.Useastrawtogentlyblowintothegraduatedcylindercontainingbromthymolblue.

3.Continueblowinguntilthebromthymolbluechangescolors.Stopblowingwhenthesolutionis
yellow.DONOTCONTINUETOBLOWBUBBLESafterthecolorchangeortherewillbetoomuch
carbondioxideinthewater.

Part2:ContainerSetUp
1.Labeleachcontainer:
Putyourinitialsonthebottom

Container1:
ElodeaLight

(EL)
Container2:
ElodeaDark

(ED)

Onthelid,writea
Y
forstartingwithyellow

2.Get2piecesofElodea.PlacetheElodeaintoContainer1and2.

3.Pourabout10mLofthepreparedbromothymolblue(nowyellow)intoeachcontainer

4.Putthecoveroneachcontainer

5.Setyourdark(
ED
)containerintothecabinetdesignatedbyyourteacher.

6.Setyourlight(
EL
)containerinthebackoftheroomintheplacedesignatedbyyourteacher

GROUPBPROCEDURE(StartingwithBlue):

Question:
Whatistheeffectof
light/dark
ontheproduction(making)ofCO
inElodea?
2

Materials:
Elodeaplants
Marker
2plasticcontainers 1graduatedcylinderwith
30mL
ofbromothymolbluesolution

(
thesolutionisatthematerialsstationpour30mLintoyourgrad.cylinder
)

Procedure:
1.Labeleachcontainer:

Putyourinitialsonthebottom

Container1:
ElodeaLight

(EL)
Container2:
ElodeaDark

(ED)

Onthelid,writea
B
forstartingwithblue

2.Get2piecesofElodea.PlacetheElodeaintoContainer1and2.

3.Pourabout10mLofthebromothymolbluefromyourgraduatedcylinderintoeachcontainer

4.Putthecoveroneachcontainer

5.Setyourdark(
ED
)containerintothecabinetdesignatedbyyourteacher.

6.Setyourlight(
EL
)containerinthebackoftheroomintheplacedesignatedbyyourteacher