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S19We1006 Offered paper — A 1,000-year-old ani robial remedy with ant staphylococcal activity Freya Harrison’, Aled Roberts', Kendra Rumbaugh’, Christina Lee!, Stephen P. Diggle! ICentre for Biomolecular Sciences, School of Life Sciences, University of Nottingham, Nottingham, UK, Department of ‘Surgery, Texas Tech University Health Sciences Center, Lubback, USA, 35chaal of English & Centre for the Study af the Viking Age, University of Nottingham, Nattingham, UK Pre-madern societies usad combinations of natural compounds to treat microbial infections. Some of these substances are known to have measurable antibacterial or antivirulence effects leg. extracts of Allium species, honey, cxgall). However, antibacterial activity of a substance in laboratery trials does not necessarily mean the historical remedy it was taken from actually worked in tato. We identified a recipe fram the 9” Century Anglo-Saxon text Batd’s Leechbook for treating a stye: an infection of an eyolash follicie usually caused by Staphylococcus -aureys, The recipe comprises five ingredients (two Adium species, oxgall, wine, copper] that may reasonably be expected to kill bacteria or reduce virulence. We reconstructed the recipe and tested it, as well as each of the individual ingrecients, in a synthetic madel of soft-tissue infection. The full recipe = but no individual ingredient alone ~ repeatably Killed S, aureus graven in ‘established biofilms, reducing cell numbers by up to six orders ‘of magnitude. Sub-Lethal doses of the recipe interfered with ‘quorum sensing and so may have anti-virulence effects, We also demonstrate sirong antibacterial activity of the full recipe in ‘chronic MRSA wound infections i vivo, Gur results highlight the untapped potential of pre-madern antibacterial remedies tor ‘yielding novel therapeutics