S19We1006
Offered paper — A 1,000-year-old
ani robial remedy with ant
staphylococcal activity
Freya Harrison’, Aled Roberts', Kendra
Rumbaugh’, Christina Lee!, Stephen P. Diggle!
ICentre for Biomolecular Sciences, School of Life Sciences,
University of Nottingham, Nottingham, UK, Department of
‘Surgery, Texas Tech University Health Sciences Center,
Lubback, USA, 35chaal of English & Centre for the Study af
the Viking Age, University of Nottingham, Nattingham, UK
Pre-madern societies usad combinations of natural compounds
to treat microbial infections. Some of these substances are
known to have measurable antibacterial or antivirulence effects
leg. extracts of Allium species, honey, cxgall). However,
antibacterial activity of a substance in laboratery trials does not
necessarily mean the historical remedy it was taken from
actually worked in tato. We identified a recipe fram the 9”
Century Anglo-Saxon text Batd’s Leechbook for treating a stye: an
infection of an eyolash follicie usually caused by Staphylococcus
-aureys, The recipe comprises five ingredients (two Adium
species, oxgall, wine, copper] that may reasonably be expected
to kill bacteria or reduce virulence. We reconstructed the recipe
and tested it, as well as each of the individual ingrecients, in a
synthetic madel of soft-tissue infection. The full recipe = but no
individual ingredient alone ~ repeatably Killed S, aureus graven in
‘established biofilms, reducing cell numbers by up to six orders
‘of magnitude. Sub-Lethal doses of the recipe interfered with
‘quorum sensing and so may have anti-virulence effects, We also
demonstrate sirong antibacterial activity of the full recipe in
‘chronic MRSA wound infections i vivo, Gur results highlight the
untapped potential of pre-madern antibacterial remedies tor
‘yielding novel therapeutics