Nama : Ratih Sukmaresi

NIM

: 12304241005

Prodi : Pendidikan Biologi Subsidi

Homo sapiens beta-myosin heavy chain (myoF) gene,

exon 3
GenBank: L47627.1
LOCUS

HUMBMYOF01

219 bp

DNA

linear

PRI 05-OCT-

1995
DEFINITION

Homo sapiens beta-myosin heavy chain (myoF) gene, exon 3.

ACCESSION

L47627

VERSION

L47627.1

KEYWORDS

beta-myosin heavy chain.

SEGMENT

1 of 2

SOURCE

Homo sapiens (human)

ORGANISM

GI:1000860

Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.

REFERENCE
AUTHORS

(sites)

Diederich,K.W., Eisele,I., Ried,T., Jaenicke,T., Lichter,P. and

Vosberg,H.P.

TITLE

Isolation and characterization of the complete human beta-myosin

heavy chain gene

JOURNAL
PUBMED
COMMENT

Hum. Genet. 81 (3), 214-220 (1989)

2522082
Original source text: Homo sapiens (clone: lambda gMHC-1-4) blood
DNA.

FEATURES
source

Location/Qualifiers
1..219
/organism="Homo sapiens"
/mol_type="genomic DNA"
/db_xref="taxon:9606"
/clone="lambda gMHC-1-4"
/cell_type="leucocyte"
/tissue_type="blood"
/germline

intron

<1..10
/number=2

exon

11..219
/number=3

ORIGIN
1 ctcactccag gcacagccat gggagattcg gagatggcag tctttggggc tgccgccccc
61 tacctgcgca agtcagagaa ggagcggcta gaagcgcaga ccaggccttt tgacctcaag
121 aaggatgtct tcgtgcctga tgacaaacag gagtttgtca aggccaagat cgtgtctcga
181 gagggtggca aagtcactgc cgagactgag tatggcaag
//

PCR
DNA Template
CT || CACTCCAG GCACAGCCAT GGGAGATTCG GAGATGGCAG TCTTTGGGGC
TGCCGCCCCC TACCTGCGCA AGTCAGAGAA GGAGCGGCTA GAAGCGCAGA CCAGGCCTTT
TGACCTCAAG AAGGATGTCT TCGTGCCTGA TGACAAACAG GAGTTTGTCA AGGCCAAGAT
CGTGTCTCGA GAGGGTGGCA AAGTCACTGC CG || AGACTGAG TATGGCAAG
Primer Reverse
CCCACCGCTTCAGTGACGGC
Tm = 4 ( G + C ) + 2 ( A + T )
Tm = 4 ( 5 + 8 ) + 2 ( 3 + 4 )
Tm = 52 + 14
Tm = 66

Primer Forward
CACTCCAGGCACAGCCATGG
Tm = 4 ( G + C ) + 2 ( A + T )
Tm = 4 ( 5 + 8 ) + 2 ( 5 + 2 )
Tm = 52 + 14
Tm = 66