6/20/2015

Alignment[completed]

Align
Alignment

P Howtoprintanalignmentincolor
P42336PK3CA_HUMAN
P42345MTOR_HUMAN

1 MLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEES

0
60

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

61 TRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDP

0
120

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

121 VVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVP

0
180

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

181 TFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEE

0
240

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

241 AEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYC

0
300

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

301 KDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLLGYSSHQGLMGFGTSPSPAKSTLVESR

0
360

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

361 CCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHV

0
420

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

421 LSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDA

0
480

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

481 TVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLL

0
540

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

541 KMLSLVLMHKPLRHPGMPKGLAHQLASPGLTTLPEASDVGSITLALRTLGSFEFEGHSLT

0
600

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

601 QFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLL

0
660

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

661 VVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSS

0
720

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

721 MNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALI

0
780

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

781 LKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVA

0
840

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

841 LWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHK

0
900

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

901 VNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFR

0
960

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

961 DQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLV

0
1020

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

1021 SFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLR

0
1080

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

1081 VFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEAPLPSRKAALETVD

0
1140

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

1141 RLTESLDFTDYASRIIHPIVRTLDQSPELRSTAMDTLSSLVFQLGKKYQIFIPMVNKVLV

0
1200

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

1201 RHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHV

0
1260

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

1 MPPRPSSGELWGIHLMPPRILVECLLP

1261 STINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNP

**.*.:

27
1312

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

28 NGMIVTLECLREATLITIKHELFKEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETR

1313 MARDLFNAAFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQ

:::**:*:*:*:..::*:::::*:

87
1355

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

88 RLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDMVKDPEVQ

1356 TLLNLAEFMEHSDKGPLPLRDDNGIVLLGERAAKCRAYAKALHYKELEFQKGPTPA

*:**.**:*:**.:*.:*:::**

137
1411

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

138 DFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWV

1412 ILESLISINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALVA

:..::.::.*:.*.:.:*:..*:*:**.:::*

196
1462

http://www.uniprot.org/align/A2015062064DM3RNY8C

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6/20/2015

Alignment[completed]

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

197 IVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVC

1463 YDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQ

**:::..::*.:***:::

255
1496

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

256 GCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESL

1497 CCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDL

*:::*:*.*:.:.:*:::.:*::.*

293
1556

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

294 YSQLPMDCFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVN

1557 FSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYK

:**:*::.:***:::::*:::

347
1606

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

348 IRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPR

1607 LVPERREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGR

:::*:*:.:.*...**:*.*

398
1665

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

399 AARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLL

1666 LALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHM

*:::*.:::.**.*:*:*::::

456
1717

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

457 NPIGVTGSNPNKET

1718 QHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYS

**:.*.*:

470
1777

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

471 PCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRL

1778 AATEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANI

.*:.*:.:*:*.**:*.*:.

517
1825

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

518 ARDNELRENDKEQLKAISTRDPLSEITEQEKDFLWSHRHYCVT

1826 TNATTAATTAATATTTASTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYT

::.*.:*.:*.**.:.:*::*

560
1881

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

561 IPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAME

1882 VPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQ

:*::.:***.*::.**::.

600
1941

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

601 LLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQ

1942 LIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMC

::**:*::*:*::*::.::**

643
1999

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

644 YLDNLLVRFLLKKALTNQRIGHFFFWHLKSEM

2000 EHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAM

.***:::::*::***

675
2057

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

676 HNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDE

2058 MERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL

::.:..*:**..**:**::::*.::::.*:

726
2115

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

727 TQKVQMKFLVEQMRRPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNW

2116 PQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTL

.::::::::*:*:*.*:*::.:.::::*:***.

783
2174

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

784 ENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCL

2175 MGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVI

::::::*:*****.:*:::::.:*:.*:*.:

839
2228

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

840 SIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFN

2229 PLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFE

:.****...*::*::*:*:::

873
2288

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

874 SHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVK

2289 HAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLD

.:***...:::****.:.:***:******:*:.

924
2347

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

925 DDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEM

2348 RLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRIT

.*:::*****.::.::*::****::.:...::

983
2399

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

984 CYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFM

2400 CHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILD

*:...:*:*:.:::.::.::::*:*.*.::::*:

1040
2459

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

1041 KQMNDAHHGGWTTKMDWIFHTIKQHALN

2460 GVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTL

:::**.::*::*.***

1068
2519

P42336PK3CA_HUMAN
P42345MTOR_HUMAN

1069

2520 DVPTQVELLIKQATSHENLCQCYIGWCPFW

1068
2549

Youmayaddadditionalsequencestothisalignment(inFASTAformat)

Resultinformation
Query
sequences

>sp|P42336|PK3CA_HUMANPhosphatidylinositol4,5bisphosphate3kinasecatalyticsubunitalphaisoform
OS=HomosapiensGN=PIK3CAPE=1SV=2
MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLITIKHELFKEARKYPLHQ
LLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFA
IGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKH
IYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLK
LCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYSQLPMD
http://www.uniprot.org/align/A2015062064DM3RNY8C

2/4

6/20/2015

Alignment[completed]

CFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGI
YHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHC
PLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWF
SSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPL
SEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAME
LLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTN
QRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILK
QEKKDETQKVQMKFLVEQMRRPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLW
LNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLS
IGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRS
CAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDF
LIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIA
YIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQHALN
>sp|P42345|MTOR_HUMANSerine/threonineproteinkinasemTOROS=HomosapiensGN=MTORPE=1SV=1
MLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEES
TRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDP
VVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVP
TFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEE
AEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYC
KDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLLGYSSHQGLMGFGTSPSPAKSTLVESR
CCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHV
LSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDA
TVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLL
KMLSLVLMHKPLRHPGMPKGLAHQLASPGLTTLPEASDVGSITLALRTLGSFEFEGHSLT
QFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLL
VVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSS
MNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALI
LKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVA
LWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHK
VNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFR
DQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLV
SFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLR
VFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEAPLPSRKAALETVD
RLTESLDFTDYASRIIHPIVRTLDQSPELRSTAMDTLSSLVFQLGKKYQIFIPMVNKVLV
RHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHV
STINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNA
AFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGI
VLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHF
GELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEK
WTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLA
QQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWW
ERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVD
PSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQHAIATEDQQHK
QELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEA
VLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTTASTEGSNSESEAESTENSP
TPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVN
EALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVAS
KSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFG
ERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQA
WDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSL
QVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNL
SIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTL
MQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRH
PSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITC
HTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDG
VELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTLD
VPTQVELLIKQATSHENLCQCYIGWCPFW

Dateofjob
execution

Jun20,2015

Jobidentifier

A2015062064DM3RNY8C(jobsarestoredfor7days)

Runningtime

12.9seconds

Identical
positions

183

Identity

6.854%

Similarpositions 342
http://www.uniprot.org/align/A2015062064DM3RNY8C

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6/20/2015

Alignment[completed]

Program

CLUSTALO

Entry

Entryname

Proteinnames

Organism Genename

P42336 PK3CA_HUMAN Phosphatidylinositol4,5bisphosphate3kinasecatalyticsubunitalpha

isoform(PI3kinasesubunitalpha)(PI3Kalpha)(PI3Kalpha)(PtdIns3kinasesubunit
alpha)(EC2.7.1.153)(Phosphatidylinositol4,5bisphosphate3kinase110kDa
catalyticsubunitalpha)(PtdIns3kinasesubunitp110alpha)(p110alpha)
(Phosphoinositide3kinasecatalyticalphapolypeptide)(Serine/threonineprotein
kinasePIK3CA)(EC2.7.11.1)

Homosapiens
(Human)

PIK3CA

P42345 MTOR_HUMAN

Homosapiens
(Human)

MTOR,
FRAP,
FRAP1,
FRAP2,
RAFT1,
RAPT1

Serine/threonineproteinkinasemTOR(EC2.7.11.1)(FK506bindingprotein12
rapamycincomplexassociatedprotein1)(FKBP12rapamycincomplexassociated
protein)(Mammaliantargetofrapamycin)(mTOR)(Mechanistictargetofrapamycin)
(RapamycinandFKBP12target1)(Rapamycintargetprotein1)

http://www.uniprot.org/align/A2015062064DM3RNY8C

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