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reagent, Brix hydrometer, and titration. Evaluating compositions of food assures the safety and
quality entering the market and warrants regulation requirements. Objectives of these
experiments are to explore different components of food through various qualitative and
quantitative techniques, discover how the components of food related to food quality and safety,
and practice various laboratory apparatus.
MATERIALS AND METHODS
Part 1: Enzymatic Browning in Apples
Took 6 pieces of sliced apple and placed 1 piece into a pedri dish for each slice to be
saturated in different solutions: ddH2O, 0.2% ascorbic acid, 0.2% catechol, 0.1% EDTA, and
0.2% sodium metabisulfite. After the apple slices were covered in the solutions for 30 minutes,
observed the physical appearance of the apple, shown in Figure 1. Rated and recorded the
browning of the apple from 1 to 5 (1 meaning not brown and 5 meaning extremely brown) in
Table 1.
Part 2: Detection of Carbohydrates in Food using Qualitative/Quantitative Techniques
Identification of starch using Lugols Reagent
25 mL of water control, glucose solution, potato juice, maltose solution, table sugar
solution, and whole milk were poured into a measuring cylinder and each solution was placed
into 50 mL beakers that were labeled WC, GS, PJ, MS, SS, and M respectively with a permanent
marker. Pipetted 2 mL of each solution into test tubes labeled WC, GS, PJ, MS, SS, and M
respectively with a permanent marker and added 1 drop of Lugols iodine solution to each test
tube. Recorded observations are showcased in Table 2.
Test for Reducing Sugar using Benedicts Reagent
1 mL of water control, glucose solution, potato juice, maltose solution, table sugar
solution, and whole milk were pipetted into 6 test tubes labeled WC, GS, PJ, MS, SS, and M
respectively with a permanent marker. Then 30 mL of Benedicts solution was poured into a 50
mL beaker by using a measuring cylinder. 5 mL of Benedicts Reagent was pipetted to each test
tube labeled WC, GS, PJ, MS, SS, and M. Test tubes were placed into a beaker with water and
heated until water boiled with a Vortex device for 10 minutes. 5 minutes after the test tubes were
removed from Vortex, observations and negative or positive results of reducing sugars were
recorded in Table 3.
Determination of the sugar content of selected beverages using a hydrometer
250 mL of deionized distill water (water control) was poured into a measuring cylinder
and gently inserted the Brix hydrometer into the solution. The degree of Brix was recorded in
grams in Table 4 and repeated these steps with Sprite, grape juice, apple juice, and orange juice.
Part 3: Determination of Milk Quality Using Various Parameters
Temperature and pH of milk were evaluated by using a thermometer and pH meter
respectively as well as smell and color of the milk (recorded in Table 6 and 7). 10 mL of milk
sample, 50 mL of ddH2O, and 2 drops of Phenolphthalein were added into a volumetric flask and
titration was performed with sodium hydroxide (NaOH, 0.1N) until pink endpoint was attained.
The amount of milk in mL and NaOH, 0.1N in grams used to titrate was recorded in Table 6.
This procedure was repeated and recorded 2 more times.
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the Food and Drug Administration (FDA), the sugar amounts displayed on the nutrition facts
panel are rounded by 0.5 g (2013), thus, can be inaccurate. In conclusion, its important to use a
hydrometer to test for quality assurance in beverages to maintain consistency; and it is needed to
continuously monitored and controlled on production line during the processes (Shachman
2004).
Manufacturing high quality milk is vital for consumers health. There are several
different parameters used to assess quality of milk: temperature, smell, color, pH, and percent of
lactic acid. Since milk is composed of 87% water, temperature will need to be monitored to
assure quality and safety of the product and prevent bacteria growth. The aroma of milk is
extremely important as well to guarantee no presence of rancidity. According to Food and
Agriculture Organization (FAO), reference values of milk has a pH level of 6.6-6.7 with acidity
of 0.16-0.18%. pH and percent of lactic acid are key indicators to determine the amount of
bacteria producing acid; the lower the pH, the higher amount of bacteria caused by the utilization
of lactose sugar. This ultimately causes protein denaturation and presence of milk curdles appear.
Results in Table 7 shows the pH levels, temperatures, smells, and colors of the 3 samples. pH
levels were in normal ranges (shown in Figure 9) with pH levels of 6 as well as normal ranges of
temperature, averaging 17.25C. The aroma of milk did not smell rancid and the color looked
standard. Results in Table 6 shows an average of 0.206% lactic acidity in 3 milk samples
completed through the process of titration with NaOH, shown in Figure 10. According to FAO,
the first clotting of milk is due to acid development which can first be seen at 0.21-0.23% lactic
acid. It is important to understand the relationship between these parameters and their normal
reference ranges; these parameters ensure safety and high quality of milk. Meeting consumer
expectations will ensure the continuation of a successful and stable dairy industry.
CONCLUSION
It is essential to understand food components and their chemical make up. Macronutrients
have many special functions in foods: they can be precursors to flavors and pigments, thickening
and bulking agents, sweeteners, and aid in natural chemical reactions. Comprehending different
chemical structures will allow food scientists to determine which tests should be achieved. These
food assessments performed require technical and analytical skills to ensure standardized quality
and safety for consumers as well as any food alterations that occurs along the process.
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REFERENCES
Bronner R. 1975. Simultaneous Demonstration of Lipids and Starch in Plant Tissues. Stain Tech
50(1): 1-4. DOI: 10.3109/10520297509117023
Dharmadhikari M. 1996. Determining Residual Sugar Using a Hydrometer. Vineyard & Vintage
View 11(6): 5.
Engelking C. 2015. Genetically Modified Non-Browning Apples Are Approved in the U.S.
Discover 26: 56-57.
Evans EC, Yakubu G, Bello T. 2013. EVALUATION OF ASCORBIC ACID AND SODIUM
METABISULPHITE AS INHIBITORS OF BROWNING IN YAM (D. rotundata) FLOUR
PROCESSING. Annals: Food Sci and Tech 14(2): 247-260.
Food and Agriculture Organization of the United Nations. Milk testing and Quality Control.
Available from: http://www.fao.org/ag/againfo/resources/documents/MPGuide/mpguide2.htm.
Accessed 2016 February 11.
Food and Drug Administration. 2013. Guidance for Industry: A Food Labeling Guide (7.
Nutrition Labeling; Questions G1 through P8). Available from:
http://www.fda.gov/Food/GuidanceRegulation/GuidanceDocumentsRegulatoryInformation/Labe
lingNutrition/ucm064894.htm. Accessed on 2016 February 21.
Kunz T, Lee V, Schiwek, V, Seewald T, Methner F-J. 2011. Glucose a Reducing Sugar?
Reducing Properties of Sugars in Beverages and Food. Brewing Sci 64: 61-67.
Lambrecht HS. 1995. Sulfite Substitutes for the Prevention of Enzymatic Browning in Foods.
American Chemical Society 600: 313-323. DOI: 10.1021/bk-1995-0600.ch024
Shachman M. 2004. Brix The Basics. In: Schachman M, editor. The Soft Drinks Companion:
A Technical Handbook for the Beverage Industry. Boca Raton: CRC Press. p 1-12.
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APPENDIX
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Figure 1: Results of slice of apple in distilled water, ascorbic acid, catechol, EDTA, and
sodium metabisulfite (from left to right) after 30 minutes.
Photo Courtesy of Jenny Nguyen from the Food Science and Nutrition Laboratory.
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Figure 2: Results of 2 mL distilled water, glucose solution, potato juice, maltose solution,
table sugar solution, and whole milk (from left to right) with 1 drop of Lugols iodine.
Photo Courtesy of Jenny Nguyen from the Food Science and Nutrition Laboratory.
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Figure 3: Results of 1 mL distilled water, glucose solution, potato juice, maltose solution,
table sugar solution, and whole milk (from left to right) with 5 mL of Benedicts Reagent
after heated on boiling water for 10 minutes.
Photo Courtesy of Jenny Nguyen from the Food Science and Nutrition Laboratory.
Figure 4: Results of hydrometer in 250 mL distilled water, Sprite, grape juice, apple juice,
and orange juice (from left to right).
Photo Courtesy of Jessica Lopez from the Food Science and Nutrition Laboratory.
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Figure 10: Sample 1, 2, and 3 of milk after titration with 0.1N NaOH.
Photo Courtesy of Jenny Nguyen from the Food Science and Nutrition Laboratory.
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Deionized
Distilled
Water
(Control)
3
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Catechol
EDTA
Sodium
Metabisulfite
Observation
Clear yellow
Clear yellow
Opaque violet
Pale yellow
Pale yellow
Result
(Positive [+] or Negative [-])
+
-
Milky white
300
Ascorbic
Acid
Orange
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Density (g/mL)
250
0.00
250
250
250
250
0.14
0.14
0.10
0.11
Volume (mL)
-
Density (g/mL)
-
350
240
240
240
0.11
0.17
0.12
0.10
V2 (mL)
(Volume of Sample
used)
10 mL
10 mL
10 mL
% of Lactaid Acid
0.20%
0.20%
0.22%
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pH
6
6
6
10 Pts
10 Pts
10 Pts
5 Pts
2 points will be deducted for each error and a score a zero (0) will be issued for any lab
report that has been plagiarized. Line numbers are to be inserted into the document at
all times.