Pce 210sme 503

PCE-210SME Ver. 5.
03
FULL AUTOMATIC BLOOD CELL COUNTER
PCE-210
Installation Instruction &
Maintenance Service Manual
For safe use of PCE-210, please read this Installation Instruction

Maintenance Service Manual well and understand function &
operation.
Keep this Installation Instruction & Maintenance Service Manual with
you to refer anytime necessary.
PCE-210SME Ver. 5.03
FULL AUTOMATIC BLOOD CELL COUNTER
PCE-210
Installation Instruction & Maintenance Service Manual
Contents
1 SAFETY NOTES TO USERS
2 COMPONENTS & FUNCTION OF THE UNIT
2-1FRONT VIEW
2-2REAR VIEW
2-3RIGHT SIDE VIEW
2-4HEMOLYSYS REAGENT HOLDING SPACE
2-5MAINTENANCE SPACE OF LIQUID CIRCUIT
3 INSTALLATION
3-1OPENING CARTON BOXES
3-2ACCESSORIES
3-3REQUIREMENTS
3-4INSTALLATION PROCEDURE
3-4-1Connecting power code
3-4-2Connecting reagents
3-4-3Setting print paper
11
3-5TURNING POWER ON
12
3-6FILLING OF REAGENTS
12
3-6-1Filling of diluent
12
3-6-2Filling of hemolyzer
12
3-6-3Filling of cleaning solution
12
4 ELECTRIC CIRCUIT
13
4-1BLOCK DIAGRAM
13
4-2THE WHOLE WIRING DIAGRAM
14
4-3LIST OF ELECTRIC CIRCUIT BOARDS
15
C.1
4-4ADJUSTMENTS OF BOARD
16
4-4-1Power board
16
4-4-2 Pressure sensor board
17
4-4-3Printer I/F board
18
4-4-4Panel switch board
18
4-4-5CPU board
19
4-4-6I/O board
20
4-4-7LCD board
22
4-4-8Wave board
23
4-4-9Power switch board
25
4-4-10Probe board
25
4-4-11WBC detection board
26
4-4-12RBC detection board
27
4-4-13+5V/+12V switching regulator
28
4-4-14Check list
29
5 LIQUID CIRCUIT
30
5-1THE WHOLE PIPING DIAGRAM
30
5-2LIST OF ELECTRIC PARTS FOR LIQUID CIRCUIT
30
5-3FUNCTION OF LIQUID CIRCUIT
31
6 SUB SELECTION
33
6-1 FACTORY ADJUSTMENT
35
6-1-1 SYRINGE VOLUME
35
6-1-2 SUCTION VOLUME
35
6-1-3 FUCTORY CALIBLATION
36
6-1-4 ACTION TIME
36
6-1-5 MOVEMENT ADJUST
36
6-1-6 DISC. VOLUME
37
6-2 FACTORY CONFIRMATION
38
6-2-1 Hgb UNIT
38
6-2-2 SUCTION PRESSURE
38
6-2-3 WORKING TIMES
39
6-3 BASICAL SETTING
40
6-3-1 DISCRIMINATION OUTPUT
40
6-3-2 CLEANING SOLUTION BOTTLE
40
6-3-3 INITIALIZATION
41
C.2
7 MAINTENANCE
42
7-1MAINTENANCE FOR LONG TIME STORAGE
42
7-2CHANGE OF PARTS
42
7-3 CLEANING DETECTOR
44
8 TROUBLE-SHOOTING
45
8-1 ERROR MESSAGE
45
8-2 TROUBLES IN MEASUREMENT
47
8-2-1 Problems and causes
47
8-2-2 Causes and corrective action
50
8-3 TROUBLES IN OPERATION
55
55
56
9 REFERENCE
57
9-1 MODE SWITCH
57
9-2 NOTE FOR USING EXTERNAL PRINTER
58
9-3 RS-232C OUTPUT
59
9-4 ROM REPLACEMENT
61
9-5 CLOG DETECTION
61
9-6 MEASUREMENT CONTROL FOR PRESSURE VOLUMETRIC METHOD
62
C.3
1 SAFETY NOTES TO USERS

Be sure to read "Safety Notes to Users" before you use this instrument for proper operation.
Notes and cautions stated in this section are important messages regarding safety
in order to protect probable dangers and/or damages, which might occur to users.
So, please be sure to read carefully.
Symbols and their meaning are as follows.
DANGER
If treated mistakenly, a danger to cause death or wound is
WARNIG
If treated mistakenly, a possibility to cause death or wound
CAUTION
If treated mistakenly, a danger to cause would and physical
expected to be imminent.
is expected to be imminent.
damage is expected to happen.
Prohibited Action
Necessary Action before using
No warranty applied if you
instrument.
do this action.
DANGER
Anyone must not disassemble, repair or modify the unit.
Those conducts may risk fire and electrical shock.
Do not perform any conducts other than 'MAINTENANCE'
as indicated in this User's Guide.
WARNING
Be sure to use Power Unit designated.
The use of Power Unit other than designated here might risk fire and
electrical shock.
Power Unit outlet should be independent.
If and outlet is shared with other electric devices, the outlet
heated extraordinarily and is subject to a cause of fire.
area may get
WARNING
When unplugged, it should not be pulled with a cable.
Pull a plug out by holding a plug with two fingers, or it may
Cause electrical shock or short to bring about fire.
Not to plug or unplug with wet hands.
It may cause electrical shock.
Not to damage power cords and connecting cables, etc.
Do not step on, twist or pull cords and cables.
Power cords and connecting cables get broken and this may
cause an
electrical shock and/or fire.

Not to use damaged power cords and connecting cables, etc.
This may cause an electrical shock and/or fire.
Never use other cords and cables than the designated ones.
Cords and cables with less electrical capacity may cause fire.
Stop operation when in extraordinary motion.
Shut off power and unplug a cord immediately when you
feel something
quite wrong with such symptoms as burning smell, strange sound, etc.
Take Ground.
Unless it is done, it may cause an electrical shock when
breakdown and electrical power leaking occur.
CAUTION
Do not use thinner or benzene as it may cause change of
color or shape for external stains of the unit, wipe with
soft cloth or cloth slightly wet. For heavy stains, wipe with
diluted detergent solution or with alcohol.
Do not put foreign matters such as bolts or coins inside
the unit. It may cause breakdown.
Use and operate the unit in accordance with enclosed
"User's Guide"
Handle switch keys of the Touch Panel with hand care.
Rough handling keys and handling them with a tip of sensor driver or ball
pointed pen may cause breakdown or damage.
For operation of switches and keys, do it one by one Doing two or more
switches at the same time may cause
breakdown or damage.
2 COMPONENTS & FUNCTION OF THE UNIT

2-1Front view
1.Touch panel LCD display
Displays measuring results,
messages
and operation status. Menu setting
can be done by touching the panel.
2.Pilot light
While this light is on, power of the unit
is on, even when LCD back light is off.
3.On/Off switch
When the main switch on the back is
in ON position, press the button to
turn the power to the unit.
Shutting off the unit can be
normally done by touch panel.
However, in case of emergency,
this switch also shuts off the unit,
however, cleaning is not to be done.
4.Reagent holder panel

At the time of changing reagents, Hemolyzer bottle, Cleaning solution bottle are to be
changed.
5.Suction probe
By pressing START switch button, the probe absorbs sample.
After suction, the probe draws back in the unit for dilution.
6.START switch
3
The switch starts suction of sample for measurement. The switch works only when suction of
sample is so requested and possible.
7.Printer cover
The cover is to be opened for changing print paper.
8.Paper cutter
This cutter is for print paper use. Paper can be cut by pressing the paper downward or
upward.
9.PRINT/FEED switch
PRINT: When LCD panel displays measured values, press the switch button to print out the
data. Every time the button is pressed, the printer prints out.
FEED: While the button is pressed, print paper keeps coming out.
10.LCD Angle Adjustment
This knob is for adjusting LCD angle in the range of +5 -5.
2-2Rear view
11.RS-232C connector
This is to be connected with PC for

data output.
12.MODE switch
This is to be used for setting Mode in
the unit.
13.Connecter of external printer
External Printer is to be connected.
14.Cooling fan
It keeps the temperature in the
unit stable. It operates all the time
while power to the unit is on.
15.Fuse holder
A fuse is held in.
4
16.AC inlet
To be connected with the attached power cable and the other side to be connected to AC
outlet.
17.Earth terminal
In case 3-pin type outlet can not be applied, or 2-pin type outlet has no terminal, take Earth
with this terminal.
2-3Right side view

18.Water supply joint
To be connected to water supply tube
(Blue mark).
19.Cleaning solution joint
To be connected to Cleaning solution
tube (Green mark).
20.Connector for waste solution tube &
float sensor
To be connected to a waste solution
tube(Red mark) and a float sensor of
waste solution.
21.Detector maintenance panel
Open the panel when cleaning detector.
22.Liquid circuit maintenance panel
Open the panel when changing roller
pump tube and/or pinch valve tube.
2-4Hemolysys reagent holding space

23. Heamolysis reagent nozzle
There is a hemolysis reagent
nozzle with cap in the
holding space for aspirating the
Hemolysis reagent.
5
When the Hemolysis reagent is changed, exchange the

Hemolysis reagent bottle for new one removing the
cap.
2-5Maintenance space of liquid circuit

24.Roller pump-XB1
This pump is for washing the sample aspiration nozzle.
Change the tubing for rollar pump periodically.
25.Roller pump-XB2
This pump is for aspirating the Diluent.
26.Roller pump-XB3
This pump is for discharging the waste solution.
27.Pinch valve (XB4)
This valve is for changing the discharge from
waste solution tank or counting chamber.
28.Pinch valve (XB5)
This valve is for changing the discharge from
WBC counting chamber or RBC counting chamber.
3 INSTALLATION
3-1Opening carton boxes
6
Take PCE-210 unit and its accessories neatly out of cartons.

By reference to the check list, make sure that no accessories are missing and no damages. If
any, contact your local agent immediately.
Set PCE-210 unit on a stable working table in an inspection room.Refer to 3-4 Installation
procedure
3-2Accessories
PCE-210 unit contains the following accessories.
No.
1
2
3
4
5
6
7
8
9
10
Descriptions
Instruction Manual
Power cable
Fuse (5A)
Waste solution bottle with tube & sensor (5L)
Diluent (5L)
Cleaning solution (2L)
The tubing sets for Diluent & cleaning
solution
Hemolysis reagent 'CYMET ERMA' 500ml
Printing roll
Cassettes with tube for Roller pump
3-3Requirements
CAUTION
Qty
1
1
2
1
1
1
1
1
1
3
1. Use the unit in the environment where room

temperature is in a range of 1035.
Particularly, make sure that room temperature for a
diluent will not go out of 1830 range to
secure stable results. In addition, install the unit in a
place 30%80% so that the unit is not subject to
dew.
2. Install the unit on a flat and sturdy table that is not
subject to vibration.
3. For installation, avoid the place that is subject to
direct sun, high humidity and water-splashing near
a sink etc.
4. Do not put a container containing reagent, water,

etc. on the unit. If the unit is drenched, it may cause
breakdown.
5. Install the unit in a place where no corrosive gas

such as chlorine gas.
6. Do not use the unit in a dusty place, as it is a

precision instrument to measure micro particles.
7. Electricity consumption is approx. 180, voltage is +/-10% against the

standard voltage. Do not use the same power outlet that is also used for airconditioner, refrigerator, micro-wave cleaning instrument and constanttemperature bath etc. which generate noise.
Also, keep the unit about 24m away from those units.
8. Do not plug in or unplug for the purpose of starting and/or ending operation.
3-4Installation procedure
3-4-1Connecting power code
DANGER
Do not connect ground cable to a plumbing line such as
Gas
pipe.
WARNING
Be sure to take a proper grounding for safety.
CAUTION
After you have confirmed that On/Off switch on front panel is
in Off position, then connect electric cables and wires.
Be sure to use an independent power source for the unit.
Particularly, use the other wall outlet for devices that generate
noise.
Put a connector of the attached power

cable into AC outlet on the back
panel, then push a plug at the other
end of the cable in wall outlet.
The end of a plug is equipped with
earth terminal. In case that ground is
not obtained by the terminal, be sure
to apply ground terminal on the rear
panel of the unit for securing
grounding.
3-Pin type outlet
2-Pin type outlet
Tak e a 2 - P i n
Use the
plug adaptor
power cable
out of the
with 2-pin
power cable
adaptor and
connect
ground cable
to an outlet.
and put a
plug into an
outlet.
grounding
is
automatically
done.
3-4-2Connecting reagents
CAUTION
Be careful not to let contamination in ground reagent.
Be careful not to let ground tube bend over.
Try to put a diluent solution bottle on the same height as the unit, as much as
possible.
Try to put a waste solution bottle on the same height as the unit,
as much as possible.
Waste solution bottle Cleaning solution bottle

Diluent bottle
10
1. Take off rubber cover over a joint on the right side panel.
2. Connection of diluent bottle
Connect a diluent bottle tube to a joint (blue)on the right side panel, then connect the
other end of the tube to the joint of a diluent bottle.
3. Connection of Cleaning solution bottle
Connect the reagent nozzle tube (green) to the joint (green) in the reagent holder inside
Front panel, then connect the other end of the nozzle tube to a Cleaning solution bottle.
4. Connection of waste solution bottle
Connect a waste solution bottle tube to a joint (red)on the right side panel, then connect
the other end of the tube to the joint of a waste solution bottle.
5. Connection of float sensor
Connect float sensor cable to the connector on the right side panel.
6. Connection of Hemolyzer bottle
Connect the reagent nozzle tube (yellow) to the joint (yellow) in the reagent holder
inside Front panel, then connect the other end of the nozzle tube to a Hemolysis
11
reagent bottle.
12
3-4-3Setting print paper
1.
Pull out the ratch of printer cover toward you while pushing printer cover stopper downward.
Printer cover can be removed.
Paper cutter
Paper discharge
Cover
stopper
2.
Pull printer lever down toward you. Insert the top end of a printer roll into the slot of print
paper insertion.
Paper holding lever
Roll paper holderPaper insertion
3.
After print paper comes out of print head, put printer lever back to its position. When you do
so, make sure that print paper is set straight.
Paper holding
lever
FWD
4.
Pull the end of print paper out of print paper outlet, then push the printer cover back in while
making sure that print paper will not get caught. For setting printer cover, put the edge of
down part of printer cover in the notch, then push the upper part in.
13
3-5Turning power on
Turn power to the unit on.
1.
Turn on On/Off switch on the front panel

of
the
mode
unit.
is
Automatic
activated
water-supply
and
WATER
SUPPLY appears on display. Selfdiagonotic of water-supply and circuit

automatically begins.
2.
Upon
the
completion
water-supply,
of
automatic
DATA
DISPLAY appears on display and the

unit is ready to measure. If abnormalities
are found at self-diagnosis, message
appears on display.
3-6Filling of reagents
On the occasion of installation of the unit and/or change and/or refill of

reagent, be sure to take this operation. Unless this operation is taken,
measurement results may not be obtained correctly.
Before you start operation, confirm that diluent bottle is full of diluent, cleaning
solution bottle is full of cleaning solution, and Hemolysis reagent bottle is full of
CYMET ERMA respectively, then waste solution bottle is empty.
3-6-1Filling of diluent
Touch EXTEND key to access to
EXTENTION menu screen, then touch
WATER key.
3-6-2Filling of hemolyzer
Touch EXTEND key to access to
EXTENTION menu screen, then touch
HEMOLYZER EXCHANGE key.
14
3-6-3Filling of cleaning solution

Touch EXTEND key to access to EXTENTION menu screen, then touch WASH key.
Touch RETURN key after finishing all operation to close EXTENTION menu.
15
4 Electric Circuit
4-1Block Diagram
16
4-2The Whole wiring Diagram
17
4-3List of electric circuit boards

No.
Board name
Function
PC-1901BPowerBoard
Generation & supply of power
PC-1802APressBoard
Pressure sensor
N02-001C Printer I/F Board
Printer I/F
PC-1904PanelBoard
Panel switch
PC-1905CCPUBoard
Control over the unit
PC-1906BI/OBoard
Drive sensor I/F
PC-1907CLCDBoard
LCD & Touch panel I/F
PC-1908BWAVEBoard
Signal management
PC-1910Power SWBoard
Power switch
10
PC-1911ProbeBoard
Probe drive I/F
11
NC-8005C(W)
Detection of WBC signal
12
NC-8005C(R)
Detection of RBC signal
13
SNP-Z073
+5V/+12V switching regulator
18
4-4Adjustments of board
4-4-1Power board
This board supplies +5V and +12V output of each switching regulator to each circuit
board. In addition, it generates +100V, and +/-15V voltage from +12V switching
regulator.
Name of board : PC-1901B
Adjustment : To be done after switch regulator adjustment.

Set
VR1
VR2
Measuring point
Setting value
TP9 (+100V)
Dial knob for voltage adjustment
TP10 (GND.B)
To be set at +100.05V+/- 0.05V.
TP6,8 (+/-15V)
Dial knob for voltage adjustment
TP7 (GND.A)
To be set at +15.05V+/- 0.05V.
Check Point
Check point
Check voltage
TP1(+5V)-TP2(GND)
Digital 5V
+5.0+/-0.1V
TP3(+12V)-TP4(GND.P)
Drive 12V
+12.0+/-0.5V
TP6(+15V)-TP7(GND.A)
Analog +15V
+15.05+/-0.05V
TP8(-15V)-TP7(GND.A)
Analog -15V
-15.05+/-0.05V
TP9(+100V)-TP10(GND.B)
For detect 100V +100.5+/-0.5V
19
4-4-2 Pressure sensor board

This board detects suction pressure, then transduce to voltage.
Name of board : PC-1802A
Adjustment : Adjustment must be done in the way that the board is set in the unit, due to
the influence of power-supply voltage.
Set
Measuring point
Procedure of adjustment & setting value
VR1
TP1
1. Turn VR1, VR2 to right as far as it can go.
VR2
TP2(GND.A)
2. Disconnect PS1 tube of pressure sensor, then,

make TP1-voltage least with VR2.
3. Suck in PS1 with an injector at -150mmHg, make
TP1-voltage 4.00+/-0.05V least with VR1
4. Repeat 2and3, then check and confirm voltage.
5. Take then injector out of PS1 and confirm min.
voltage of TP1 remains unchanged.
*If changed, repeat 4.
6. Suck in PS1 with an injector at -150mmHg, confirm
voltage of TP1 is 4 +/- 0.005V.
*If changed, repeat 4.
7. Suck in PS1 with an injector at -30mmHg, confirm
voltage of TP1 is 800 +/- 40mV.
*If out of range, repeat 4.
Check point
Check point
TP1-TP2(GND.A)
Check voltage
Pressure signal4.00+/-0.05V
*When at -150mmHg
20
-150mmHg -20kPa
4-4-3Printer I/F board
This board converts data from CPU board and get printer to print.
Name of board : N02-001C
Adjustment : No items that need to be adjusted.
Check point : No points that need to be checked.
4-4-4Panel switch board
When panel switch is pressed, this board get printing and feeding started.
Name of board : PC-1904

Check point
Check point
Check voltage
SW1 (FEED Switch)
To feed paper
SW2 (PRINT Switch)
To start printing
21
4-4-5CPU board
This board activates program and controls over the unit.
Name of board : PC-1905C

Check point
Check point
J1(Motion mode)
Check voltage
Insert a jump socket in between 1Pin & 2Pin.
*When Flash ROM program is rewritten
insert it in between 2Pin & 3Pin.
J2,J3(applied ROM)
Insert a jump socket in between 1Pin &

22
2Pin.Flash ROM mode

*When UV-EP ROM is used, insert it in
between 2Pin & 3Pin.
J4,J5(Optional function)
Insert a jump socket in between 1Pin &

2Pin. (Standard specification)
TP1(+5V)-TP2(GND)
LOGIC 5V
+5.00+/-0.20V
TP3(+5V)-TP4(GND.P)
PRINTER 5V +5.00+/-0.20V
4-4-6I/O board
This board controls liquid circuit parts such as electric valves, pumps, motors and driving
unit according to signals from CPU.
Adjustment : Adjust this board after aging for 30min. from power ON.
Set
VR9
Measuring point
Adjustment method & setting value
TP9
Sample syringe pulse width adjustment
TP2(GND)
Adjust TP9 pulse width to 6.0+/-2.5msec

(1 cycle) with VR9.
VR10
TP10
Diluent syringe pulse width adjustment

23
TP2(GND)

(1 cycle) with VR10.
VR11
TP11
Hemolyzer syringe pulse width adjustment
TP2(GND)

(1 cycle) with VR11.
Sample syringe pulse
1 ms/div
1 v/div
6.0 msec
Diluent syringe pulse
2 ms/div
1 v/div
12.0 msec
Hemolyzer syringe pulse
24
1 ms/div
1 v/div
6.0 msec
Check point
Check point
Check voltage
TP1(+5V)-TP2(GND)
For logic 5V
5.00+/-0.20V
TP3(+12V)-TP4(GND.D)
For drive 12V 12.00+/-0.20V
TP9-TP2(GND)
Sample syringe pulse
6.0+/-2.5msec
TP10-TP2(GND)
Diluent syringe pulse
12.0+/-2.5msec
TP11-TP2(GND)
Hemolyzer syringe pulse 6.0+/-2.5msec
*Not in use for TP5-TP8

4-4-7LCD board
This board converts data from CPU to LCD signals, making letters and graphics appear
on LCD. It also transfers coordinates data of touch panel to CPU board.
25
Adjustment
Set
Menu
Measuring point
Eye inspection at
Adjustment of LCD contrast
touch panel.
To adjust by Menu operation on touch panel LCD.
4-4-8Wave board
This board selects the only wave that pass through a blood cell from signal wave amplified
by WBC, RBC detection boards, and counts number of a blood cell by size by way of A/D
Transfer and DSP Treatment. It also amplifies HGB detection signal.
26
Adjustment : *Adjust the Hgb voltage with metal cover.

Set
VR1
Measuring point
TP3
PLT Standard voltage-2 adjustment knob
TP9(GND.A)
Adjust voltage between TP3-TP9 to

4.15+/-0.01V.
VR2
TP5
PLT Standard voltage-1 adjustment knob
TP9(GND.A)

0.80+/-0.02V.
VR3
TP6
RBC Standard voltage-1 adjustment knob
TP9(GND.A)

0.40+/-0.02V.
VR4
TP7
WBC Standard voltage-1 adjustment knob
TP9(GND.A)

0.75+/-0.02V.
Set
VR5
Measuring point
TP11
HGB Off-set adjustment knob
TP16(GND.A)
Disconnect JP7 and adjust voltage between

TP11-TP16 to 0.00+/-0.01V.
VR6
TP13
HGB sensitivity adjustment knob

27
TP16(GND.A)
Connect JP7 and adjust voltage (at measuring

diluent) between TP13-TP16 to 4.00+/-0.02V
VR
TP33
ADC offset volume for adjustment
TP35(GND.A)
Adjust voltage between TP33-TP35 to0.00+/-0.02V
Check point
Check point
J1,J2 (Setting of Hgb gain)
Check voltage
HGB Gain setting:
Insert a jump socket in between 2 - 3Pin.
J3 (Selection of reset signal )
Reset signal Select:

J4 (Selection of reset signal )
Reset signal Select:

TP2(+5V)-TP4(GND)
For logic 5V5.00+/-0.20V
TP10(+15V)-TP12(GND.A)
For analog +15V+15.00+/-0.20V
TP14(-15V)-TP12(GND.A)
For analog 15V-15.00+/-0.20V
TP3-TP9(GND.A)
PLT Standard Voltage24.15+/-0.01V
TP5-TP9(GND.A)
PLT Standard Voltage10.80+/-0.02V
TP6-TP9(GND.A)
RBC Standard Voltage10.40+/-0.02V
TP7-TP9(GND.A)
WBC Standard Voltage10.75+/-0.02V
TP8-TP9(GND.A)
WBC Standard Voltage28.30+/-0.02V
TP17-TP12(GND.A)
WBC detection board output wave
TP18-TP12(GND.A)
WBC differential wave
TP19-TP12(GND.A)
WBC A/D request pulse
TP20-TP12(GND.A)
WBC A/D reset pulse
TP34-TP35(GND.A)
WBC P/H circuit wave
TP21-TP12(GND.A)
RBC detection board output wave
TP22-TP12(GND.A)
WBC differential wave
TP23-TP12(GND.A)
RBC A/D request pulse
TP24-TP12(GND.A)
RBC A/D reset pulse
TP32-TP35(GND.A)
RBC P/H circuit wave
TP25-TP12(GND.A)
PLT detection board output wave
TP26-TP12(GND.A)
PLT differential wave 1
TP27-TP12(GND.A)
PLT differential wave 2
TP28-TP12(GND.A)
PLT forming wave
TP29-TP12(GND.A)
PLT A/D request pulse
TP30-TP12(GND.A)
PLT A/D reset pulse
TP31-TP35(GND.A)
PLT P/H circuit wave
TP11-TP16(GND.A)
HGB offset voltage
TP13-TP16(GND.A)
HGB detection voltage
TP1-TP16(GND.A)
Pressure detection voltage

28
4-4-9Power switch board

By pressing the switch, power is turned on to the unit. For turning power to the unit off, it
can be done by touch panel LCD menu operation. However, in case of emergency, you can
shut off the unit by pressing the switch.

Check point :No points that need to be checked.
4-4-10Probe board
This board drives UP/DOWN motor by control from I/O board.It detects location of probe
block and transfers the message to I/O board.
29

4-4-11WBC detection board

This board flows detection electric current to WBC detection electrode. It amplifies a signal that
was detected at WBC detection part.
Name of board : NC-8005C(W)
Adjustment : VR2 is mounted in the reverse side of the board.

Set
VR2
Measuring point
TP2
WBC Sensitivity adjustment knob
TP3(GND.A)
At the time of measuring blank, confirm

that base allowance of signal is within 0.2V.
1.
Measure latex particle which you know the exact

volume.
2.
Adjust the peak of WBC histogram by VR2 to be

same volume as latex you measured.
JP
Short pin for shifting detection electric current

Short B position (center).
30
Check point
Check point
Check voltage
TP2-TP3(GND.A)
WBC amplification circuit output wave
TP4-TP3(GND.A)
WBC 1st stage output wave
4-4-12RBC detection board

This board flows detection electric current to RBC detection electrode. It amplifies a signal that
was detected at RBC detection part.
Name of board : NC-8005C(R)
Adjustment : VR1 and VR2 are mounted in the reverse side of the board.
Set
VR1
Measuring point
TP1
PLT sensitivity adjustment knob
TP3(GND.A)
Measure control blood and arrange in the

way that MPV is within assay value.
Also, at the time of measuring blank, confirm
VR2
TP2
RBC sensitivity adjustment knob
TP3(GND.A)
Measure control blood and arrange in the

way that MCV is within assay value.
Also, at the time of measuring blank, confirm
31

JP
Short pin for shifting detection electric current

Short B position (center).
Check point
Check point
Check voltage
TP1TP3(GND.A)
PLT amplification circuit output wave
TP2TP3(GND.A)
RBC amplification circuit output wave
TP4TP3(GND.A)
RBC 1st stage output wave
4-4-13+5V/+12V switching regulator

This board generates DC5V/+12V from AC power source.
Name of boardSNP-Z073
Adjustment : Switching regulator has been adjusted before shipment.

Check point
Check point
Check voltage
P1,2-P3,4,5,6(DC output connector TB2)
DC output volgate 5.0+/-0.1V
P7,8-P3,4,5,6 (DC output connector TB2)
DC output volgate 12.0+/-0.5V
32
4-4-14Check list
If you replace the power supply or the boards, please check voltage of each part
according to the following check list.
Name of Product:
PCE-210
Serial No.:_____________
ROM Ver.
Check Item
Content
Power Board
Digital 5V+/-0.1 TP 1: ________V
PC-19 01B
Drive12V+ /-0.5 TP3 : ________V

Analog+15.05V+/-0.05 TP6: ________V(VR2)
Analog-15.05V+/-0.05 TP8: ________V(VR2)
Detector 100.5V+/-0.5 TP9: ________V(VR3)
Pressure Sensor
T P 1 ( 0 mm H g ):
__________mV(VR2)
Board PC1802A
TP1 (-150mmHg)4V+/-0.05: __________V(VR1)
I/O Board
Sample Syringe 6.0+/-2.5msec TP9 : _________msec(VR9)
PC1906B
Diluent Syringe 12.0+/-2.5msec TP10: _________msec(VR10)

Lyse Syringe 6.0+/-2.5msec TP11: _________msec(VR11)
Wave Board
4.15V+/-0.01 TP3: _______V(VR1)
PC1908B
0.80V+/-0.02 TP5: _______V(VR2)

0.40V+/-0.02 TP6: _______V(VR3)
0.75V+/-0.02 TP7: _______V(VR4)
0.00V+/-0.01 TP11: _______mV(VR5)
4.00V+/-0.02 TP13: _______V(VR6)
0.00V+/-0.02 TP33: _______mV (VR7)
WBC Board
Adjust the peak of WBC histogram by VR2 to be same volume as latex particle.
NC-8005C
Wave of base signal during blank measurement: _______V
RBC Board
*MPV value of control blood is within the range of assay value.(VR1)
NC-8005C
*MCV value pf control blood is within the range of assay value.(VR2)

Wave of base signal during blank measurement: _______ V
Aspiration pressure
Time to reach the aspiration pressure
33

action
Pressure Data: _______mmHg

Time to reach: _______msec
Hgb unit action
A/D value of blank data: _______
5 Liquid Circuit
5-1The Whole piping Diagram
5-2List of electric parts for liquid circuit

Parts
Application
Type
Name of parts
XA1
For WBC orifice discharge
2way valve
LVM11R-6 DC12V
XA2
For RBC orifice discharge
2way valve
LVM11R-6 DC12V
XA3
For WBC orifice supply
2way valve
LVM11R-6 DC12V
XA4
For RBC orifice supply
2way valve
LVM11R-6 DC12V
XA5
For WBC cell agitation
2way valve
LVM11R-6 DC12V
XA6
For RBC cell agitation
2way valve
LVM11R-6 DC12V
XB1
For suction probe cleaning
Roll pump
SR10/50-N3.2 DC12V
XB2
For water supply
Roll pump
SR10/50-N3.2 DC12V
XB3
For waste solution
Roll pump
SR10/50-N3.2 DC12V
XB4
For waste tank/cell discharge.
Pinch valve
HYN-5 DC12V
34
XB5
For WBC/RBC cell discharge.
Pinch valve
HYN-5 DC12V
XB6
For increasing pressure
Air pump
CM-15-12
XB7
- " - (Waste tank)
2way valve
LVM11R-6 DC12V
YA1
Dilution syringe IN/OUT
3way valve
LVM112-6 DC12V
YA2
Hemolyzer syringe IN/OUT
3way valve
LVM112-6 DC12V
YA3
Switch for supply & cleaning
3way valve
LVM112-6 DC12V
5-3Function of liquid circuit

XB1 pump and syringe
XB2 pump
35
XB3 pump
XB6 pump
36
6 SUB SELECTION
In this unit, special operations that are not normally in use are separate as SUB SELEC TION,
and it does not appear normally. For having SUB SELECTION menu appear, the special
operation is needed.Please do either METHOD A or METHOD B as mentioned below.
METHOD A.
Press the clock display 3 times in stand-by screen.
METHOD B.
Put 1 Switch of MODE SWITCH on the back panel
in ON position, then turn the power to the unit on.
Press MENU key to display SELECT MENU.

8 SUB SELECTION, which is not shown in normal
37
mode, appears.
If you press PRINT button while SUB SELECTION is

displayed, set values are printed by the internal
printer.
CALBRATION / INSTRUCTION MANUAL 5-1
BRIGHT ADJUST / INSTRUCTION MANUAL 5-3-6
PRINTING MODE / INSTRUCTION MANUAL 5-4-1
OUTPUT SETTING / INSTRUCTION MANUAL 5-4-3
WBC MODE / INSTRUCTION MANUAL 5-4-4
SET AUTO CLEANING / INSTRUCTION MANUAL 5-4-6

38
NORMAL RANGE / INSTRUCTION MANUAL 5-5
GAIN / INSTRUCTION MANUAL 5-6
6-1-3 FACTORY CALIBRATION
6-1-4 ACTION TIME
6-1-6 DISC. VOLUME
When operations in SUB SELECTION finish:

If you used METHOD A to display SUB SELECTION screen, return to stand-by screen and
press the clock 3 times. If you used METHOD B to display SUB SELCTION screen, turn
DIP switch #1 OFF and turn the power off and on again.
6-1 FACTORY ADJUSTMENT
39
You may change each syringe volume by adjusting

pulse of the syringe pulse moter. PLEASE NOTE
THAT IT MAY AFFECT DATA IN CASE YOU
CHANGE PULSE BECAUSE DILUTION RATIO IS
ALSO AFFECTED.
SAMPLE SYRINGE Approx. 0.55ul/1pulse
DILUENT SYRINGE Approx. 35.53ul/1pulse
HEMORYZER SYRINGE Approx. 6.80ul/1pulse
The input range of each syringe is as below.
SAMPLE SYRINGE
10 - 70
DILUENT SYRINGE
100 - 130
HEMORYZER SYRINGE 40 - 150

You can adjust suction time for diluted samples. If
you set the time longer, the suction volume become
larger and accordingly data appears higher. PCE-210
is initially set the time to aspirate 0.25ml each for
WBC and RBC. The input range is as below:
WBC SUCTION VOLUME 6.0 - 15.0
RBC SUCTION VOLUME 8.0 - 16.0
6-1-3 FUCTORY CALIBLATION

You can manually input calibration values in case
measurement data of control blood is out of range
from expected values etc. If you want to change the
calibration values in this MENU, firstly reset the
values set by user to 100%. The input range of
calibration
40
values
is
25.0
200.0%
for
each
parameter.
6-1-4 ACTION TIME
MEASUREMENT START TIME
Time from the end of stirring in WBC cell to the start

of counting during measurement. Initial value 0.
Setting range 0-1000.
PROVE WASHING TIME
Time of filling diluent solution to the nozzle washing

block during nozzle cleaning. Initial value 2000.
Setting range 1000-3000.
STIRRING TIME
Time of stirring sample during measurement. It
indicate time of air venting from the bottom of the
measurement cell. Initial value 400. Setting range
200-1000.
6-1-5 MOVEMENT ADJUST
Just after making operation by using these menus,
there may be change in volume of diluent solution
inside the measurement cell or in volume of solution
on the tip of sample nozzle. Therefore, make sure
either to take blank measurement or to operate water
supply before starting sample measurement.
PROBE UP/DOWN ACTION

The sample nozzle (probe) moves up and down one time.
SAMPLE SYRINGE
The sample syringe moves up and down one time.
DILUENT SYRINGE
The diluent syringe moves up and down one time. Be aware of the diluent solution
discharged from the sample nozzle.
HEMOLYZER SYRINGE
41
The lysing syringe moves up and down one time. The lysing solution is discharged to the
WBC cell by this operation.
PROBE ACTION
A continuous sample nozzle action just as same as the action during measurement is
operated. It stops by pressing the key again when the nozzle is in the front.
6-1-6 DISC. VOLUME

Input range of setting value is between 1 and 200.
The unit mentioned as ad in screen stands for 1bit
volume of installed in AD converter of this device.
Clogging Detection Function

In every measurement, this function observes fluctuation of measured voltage between
electrodes of the detection part. When the device detect clogging,, its automatic clog
removing function works and repeats measurement. If the clogging is removed within 2
times of re-measurement, it normally shows the measurement data in display. However, if
the clogging continues, Clogging Message will appear after finishing measurement.
The following is a note for setting value.

The initial set value is for detecting even a tiny clogged situation that actually does not affect
quantity of blood cell but may affect cell volume slightly. If you require higher accuracy in WBC
distribution, Hct, MCV, and MPV, please use the device with the initial set value. To make the
device less sensitive for clogging, please set the bigger value (Around 50). However, please be
noted there may be a little lack of accuracy in cell volume related parameters, if you set the
value larger.
42
6-2 FACTORY CONFIRMATION
6-2-1 Hgb UNIT

Voltage of the Hgb Measurement unit (Value of AD
converter) is displayed. The standard value is 820 +/20. 4.0V equals to 820. Please refer to the service
manual for details.
6-2-2 SUCTION PRESSURE

It shows pressure inside the negative pressure tank in
the liquid circuit. It normally takes about 2 sec to
reach 150mmHg. If it takes extremely longer time to
reach 150mmHg, the pump may be deteriorated. Or
if you see the pressure starts decreasing immediately
after reaching the certain point, there may be a
leakage somewhere in the liquid circuit.
43
-150mmHg
Normal
Deteriorate of Pump tube
Leakage
2000msec
The following drawing show area which suction pressure reaches or affects ,when you operate
"SUCTION PRESSURE" check.
44
6-2-3 WORKING TIMES

Counters in this screen show the number of each
performance operated so far. By those counters, you
can check how many times the instrument has been
working. The number of the counters can not be
manually changed but you can reset to 0. Press the
item you wish to reset. After the display color is
reversed, press CLEAR key. The countable range for
each item is 0-99999.
6-3 BASICAL SETTING

If you make the discrimination output ON, border
lines showing discrimination position appear in
45
histograms(WBC, RBC, and PLT). Those lines also appear in print-outs by the internal printer but not
in print-outs by the external printer nor in external output.
6-3-2 CLEANING SOLUTION BOTTLE

You can select volume of cleaning solution bottles.
Make sure to set new
cleaning solution after making change in the
selection.
46
6-3-3 INITIALIZATION
If you press YES at this menu, all the stored
sample data, input header, calibration values, normal
range, and all the set values in SUB SELECTION
menu are initialized. Also, counters of WORKING
TIMES are reset. Please make sure to take all the
necessary data before if you wish to use this menu.
47
7 MAINTENANCE
7-1Maintenance for long time storage
CAUTION
Instrument may be badly damaged if you store it for long period
without disconnecting reagents.
If you keep PCE-210 inoperated for long time, remaining diluent solution may
crystallize or contaminate in internal parts of the instrument and could be a cause
of clogging. To avoid any defect in the device, please refer to 5-3-4 Long time
storage in Operation Manual and refill distilled water to clean the liquid circuit..
Make sure to drain completely after it.
7-2Change of parts
CAUTION
Replace or clean roller pump tube once per 6 months or 3000
measurement, whichever comes first.
Make sure to use roller pump tube supplied by manufacturer .
1. At READY SCREEN display, touch MENU

key to get access to SELECT MENU display.
At SELECT MENU screen,
touch MAINTENANCE key to
get access to
CHANGE TUBING display.
2. Touch STEP 1 key. Liquid in the unit is
drained, After liquid has been drained, open liquid circuit maintenance panel on the right side
of the unit, then disconnect a joint which is connected with a roller pump tube.
48
3. Push both notches in horizontal position in with two fingers and pull the roller cassette the
forward you.
Push both notches in the back in

vertical
position
comparison
with
(90angle
the
notches
in
in
above 3.) in with two fingers, then

take out cassette cover and take out
a tube.
4. Push a tube deeply into the inside cassette, check and see if the tube has been firmly held
in, then put a cassette cover back in. After you have heard the sound of check the cassette
had been surely set, then connect the tube to a joint.
5. Touch STEP 2 key. Conform that a pump works.

6. Touch STEP 3 key. Water supply starts. Make sure of no leakage, then close side panel.
49
50
7-3 Cleaning Detector

Clean the measurement related part by following process, when frequent clogging or
unstable
Hgb value is seen.
1.
Display stand-by screen and touch

EXTEND key.
2.
Open the reagent door in the right side of instrument, pour 5-6 drops of hypochlorite for
both WBC & RBC measurement cell.
CAUTION
Please use 5-6% of hypochlorite. DO NOT use home-use
detergent nor breech, which may cause trouble in analyzer.
Please do not leave the device after pouring hypochlorite and
proceed to No. 3 below.
3.
Close the reagent door and touch

WASH key.
CAUTION
Make sure to touch WASH key
after closing the reagent door.
4.
Go back to stand-by screen by touching RETURN key after completing all operation.
51
8 TROUBLE-SHOOTING
8-1 Error message
By way of the self-diagnostic system of the
unit,
if
there
are
some
troubles,
error
messages appear on LCD display. Treat

properly in accordance with the guidance
against each error message.
MESSAGE
STORED SAMPLES ERROR
Stored samples now 200.
CAUSE
Memory
is
TREATMENT
over Touch
200.
CONFIRM
key
to
delete
message. Delete stored sample data

since total number of data that can be
Delete Data.
stored is 200.
WASTE SOLUTION CHECK
Waste solution full.
Waste solution in Dispose of waste solution in waste
solution
the tank is full.
Discard it.
bottle,
then
touch
CONFIRM key.
NO PAPER
No paper in printer.
Print paper is not Set
print
paper
and
touch
CONFIRM key.
set.
Supply paper.
PRINTER ERROR
Head-up lever of Pull down the lever, then touch
No right action.
the printer is in up
Check printer action.
position.
CREANING SOLUTION CHECK

Cleaning solution short.
Cleaning
solution Replace old cleaning solution bottle
is running short.
Hemolysys reagent short.
with
new
one,
then
touch
CONFIRM key.
Add cleaning solution.

HEMOLYSYS REAGENT CHECK
CONFIRM key.
Hemolyzer
running short.
is Replace old hemolyzer bottle with
new one and touch CONFIRM key.
Add hemolysys reagent.

CHECK PROBE UP/DOWN
The probe is not Touch CONFIRM key to delete
52
No right action.
properly
Check probe action.
performing up and
message.
down operation.
CHECK PROBE FRONT/REAR
The probe is not Touch CONFIRM key to delete
No right action.
properly
message.
Check probe action.
performing
front
and
rear
operation.
MESSAGE
CHECK SAMPLE SYRINGE
CAUSE
The
TREATMENT
sample Touch CONFIRM key to delete
No right action.
syringe
is
Check syringe action.
properly
not message.
performing.
CHECK DILUENT SYRINGE
diluent Touch CONFIRM key to delete
The
No right action.
syringe
properly
not message.
is
performing.
hemolyzer Touch CONFIRM key to delete
CHECK HEMO. REAGENT SYRINGE
The
No right action.
syringe
properly
not message.
is
performing.
CHECK WBC CLOGGING
Measuring part clogged.
WBC detector has Touch CONFIRM key to delete

got clogged.
message. Take cleaning action and

check
Remove clogging.
the
remaining
volume
of
hemolyzer.
CHECK RBC CLOGGING
Measuring part clogged.
RBC detector has Touch CONFIRM key to delete

got clogged.
message. Take cleaning action.
Remove clogging.
DILUENT CHECK
Diluent not full.
Diluent is not full Refill diluent and check if any tube

in Liquid circuit.
key.
Add diluent
CHECK WBC LEAK
bending etc. then touch CONFIRM
Suction pressure Check samples and also check and
Sample suction pressure
in
WBC
Liquid see if any tube bending etc. then,
Not increasing.
circuit
does
Check sample.
reach
the
not touch CONFIRM key.

fixed
level.
CHECK RBC LEAK
Suction pressure Check samples and also check and

53
Sample suction pressure
in
Not increasing.
circuit
does
Check sample.
reach
the
RBC
Liquid see if any tube bending etc. then,

not touch CONFIRM key.
fixed
level.
INPUT ERROR
Abnormality
Set values out of range.
set
values
Input values again.
memory
in
for Touch
CONFIRM
key.
Take
at initialization process for the unit, then

the input data again.
unit is found.
CHECK PINCH VALVE
Drainage is abnormal.
Drainage
is Touch CONFIRM key. Please check
abnormal.
if there are no adhesions in tubes of

XB4 and XB4 pinch valves.
Check tube adhesion.
8-2 Troubles in Measurement

PROBLEMS
Blank count can not be 0.
CAUSES
Ref
Shortage/Poor quality of Diluent
A-1
Poor quality of Hemolyzer
A-3
Defect of Water supply circuit
A-4
Stains/bubbles of Cell
A-8
Clogging/stains/bubbles of Orifice
A-9
Stains/bubbles of Electrode
A-11
Poor setting of Electrode
A-12
Stains/bubbles of probe cleaning
A-13
Bubbles when diluent discharge
A-22
Bubbles in Water supply circuit
A-23
Air leakage of Detection part
A-43
Influence by inner noise
A-45
Influence by outer noise
A-46
Defect of connection cable
A-47
Blank count can be 0, but
A-9
measured value is high.
Defect of Orifice
A-10
Position of probe clean
A-14
Defect of suction volume
A-16
54
Measured value is low.
Mis-calibration
A-17
Defect of WBC sensitivity adjust
A-19
Defect of RBC sensitivity adjust
A-20
Defect of PLT sensitivity adjust
A-21
Bubbles in Hemolyzer circuit
A-24
Poor agitation
A-25
Defect of Hemolytic time
A-26
Defect of Hemolyzer volume
A-27
Defect of Sample
A-31
Defect of Sample syringe volume
A-33
Defect of Diluent syringe motion
A-34
Defect of Sample syringe motion
A-35
Defect of Hemolyzer syr. motion
A-36
Bent/clogging of wiring tubes
A-39
A-9
Defect of Orifice
A-10
A-14
Defect of suction volume
A-16
Mis-calibration
A-17
A-19
A-20
A-21
PROBLEMS
CAUSES
Ref
Measured value is low.
Poor agitation
A-25
continued
A-27
Fluid leakage of HGB block
A-28
Defect of Sample
A-31
Mis-suction of sample
A-32
Defect of Sample syringe volume
A-33
A-35
55
Leakage of wiring tubes
A-40
Loose tube joint
A-41
A-43
Air leakage of Waste/Supply tank
A-44
A-8
A-9
A-11
A-13
A-14
Clogging of filter
A-15
A-19
A-20
A-21
A-23
Poor agitation
A-25
A-26
A-27
A-32
Defect of Diluent syringe motion
A-34
A-35
Defect of Hemolyzer syr. motion
A-36
A-39
A-40
Loose tube joint
A-41
A-43
A-45
Infulence by outer noise
A-46
Abnormality of WBC
Proor quality of Hemolyzer
A-3
Classification.
Defect of Hemolyzer flow
A-6
A-9
Defect of Orifice
A-10
A-11
A-19
Poor agitation
A-25
A-26
A-27
Defect of Sample
A-31
A-45
A-46
Poor reproducibility.
56
PROBLEMS
CAUSES
Ref
"CHECK CLOG" frequently
Poor quality of Diluent
A-1
appears.
Poor quality of Cleaning solution
A-2
Poor quality of Hemolyzer
A-3
A-8
A-9
Defect of Orifice
A-10
A-11
A-13
Defect of pressure board adjust
A-18
Defect of Solenoid valve
A-38
A-39
"CHECK
DILUENT"
appears.
Fluid can not be discharged.
All and every data are 0.
frequently Defect of Water supply circuit
A-4
Defect of pressure board adjust
A-18
A-23
Fluid leakage of Cell
A-30
Defect of Pump/solenoid valve
A-37
A-39
A-40
Loose tube joint
A-41
A-43
A-44
A-37
A-39
A-40
Loose tube joint
A-41
Wrong piping
A-42
A-44
A-32
A-35
Defect of Suction probe motion
A-38
A-39
57
Reagent can not flow.
Fluid leaks and/or come out.
A-40
Loose tube joint
A-41
Wrong piping
A-42
A-47
Defect of Water supply circuit
A-4
Defect of Cleaning solution flow
A-5
Defect of Hemolyzer flow
A-6
Fluid leakage of Suction probe
A-29
Fluid leakage of Cell
A-30
A-37
Defect of Suction probe motion
A-38
A-39
A-40
Loose tube joint
A-41
Wrong piping
A-42

CAUSES
A-1
Poor quality of Diluent.
CORRECTIVE ACTION
In case of bubbles in water supply tube,
supply water by EXTENDWATER .
In case of NO Diluent, after changing it,
supply water by EXTENDWATER .
In case that bubbles/floating materials are
found, after changing Diluent, supply water by
EXTENDWATER .
A-2
Poor quality of
Cleaning solution.
found, after changing Solution, take cleanng

action by EXTENDWASH .
A-3
Poor quality of
Hemolyzer.
found, after changing Hemolyzer, touch

EXTENDHEMOLYZER EXCHANGE .
A-4
Defect of Water
Supply water by EXTENDWATER then,

58
supply circuit.
confirm that supply water is in action.

Defect of Diluent syringe motion (Ref. A-34)
Defect of Pump/solenoid valve (Ref. A-37)
Bent/clog of wiring tube (Ref. A-39)
Leakage of wiring tubes (Ref. A-40)
Loose tube joint (Ref. A-41)
Wrong wiring (Ref. A-42)
Air leakage of Detection part (Ref. A-43)
Air leakage of Water/Supply tank (Ref A-44)
A-5
Defect of Cleaning
Check cleaning solution bottle. In case of NO
solution flow.
solution, replace with new solution.

Supply water by EXTENDWATER then,
confirm that supply water is in action.
Wrong piping (Ref. A-42)
Air leakage of Detection part (Ref. A-43)
A-6
Defect of Hemolyzer
Check Hemolyzer bottle. In case of NO
Flow.
Hemolyzer, replace with new one.

touch SELECT MENUMAINTENANCE
LIQUID CIRCUIT TESTSYRINGE ACTION.
and see that Hemolyzer flows into WBC Cell.
Defect of Hemolyzer syr. motion (Ref. A-36)
CAUSES
A-8
Stains/bubbles of
CORRECTIVE ACTION
Touch EXTENDWASH for cleaning.
59
A-9
Cell.
Remove Cell for disassembling cleaning
Clogging/stains/
bubbles of Orifice.
Touch SELECT MENUMAINTENANCE LIQUID

CIRCUIT TEST RBC CLOG REMOVE or WBC
CLOG REMOVE a few times.
Remove Orifice for cleaning.
Replace with new Orifice.
A-10
Defect of Orifice.
Check and see if WBC Side(100u)and RBC

Side(80u) has been set upside down.
A-11
A-12
Stains/bubbles of
Electrode.
Remove Electrode for cleaning.
Poor setting of
See that Electrode not in contact with others.
Electrode.
In case of cracks on Electrode, replace it with

new one.
A-13
Stains/bubbles of
Touch
SELECT
MENUSUB
SELECTION
probe cleaning.
FUCTORY ADJUSTMENT ACTION TIME

NOZZLEPROVE WADHIG TIME to change wash
time
Remove probe from the unit for
disasemblying cleaning.
A-14
Position of probe clean.
While suction probe is in up position, adjust

that tip of probe is on the same hight with
inside-tapered area of probe cleaning part.
A-15
Clogging of filter.
Remove Filter from the unit for claning.

Replace with new Filter.
A-16
Defect of suction volume.
Measure Control blood, then touch

SELECT MENUSUB SELECTION FACTORY
ADJUSTMENTSUCTION
VOLUMEWBC
SUCTION VOL or RBC SUCTION VOLUME to

readjust volume.
A-17
Mis-calibration.
Take Calibraton by SELECT MENUCALIBRATION

(Ref. User's guide 5-2 Calibraton)
A-18
A-19
A-20
A-21
Defect of pressure
Adjust pressure sensor board.
board adjust.
(Ref. 3-3-2 Pressure sensor board)
Defect of WBC
Adjust WBC detecton board.
sensitivity adjust.
(Ref. 3-3-11 WBC detection board)
Defect of RBC
Adjust RBC detecton board.
sensitivity adjust.
(Ref. 3-3-12 RBC detection board)
Defect of PLT
Adjust RBC detecton board.
sensitivity adjust.
(Ref. 3-3-12 RBC detection board)

60
A-22
Bubbles when
touch EXTENDWASH for cleaning.
diluent discharge.
Adjust Pulse width of diluent syringe.

(Ref. 3-3-6 I/O board)
CAUSES
A-23
CORRECTIVE ACTION
Bubbles in Water
supply circuit.
Check Diluent bottle. In case of NO Diluent,

replace with new one.
Air leak of Detection part (Ref. A-43)
A-24
Bubbles in
Touch EXTENDHEMOLYZER EXCHANGE to
Hemolyzer circuit.
remove bubbles in Hemoly.circuit to WBC cell.

Check Hemolyzer bottle. In case of NO
Hemolyzer, replace with new one.
A-25
Poor agitation.

A-26
Defect of Hemolytic time.
Touch SELECT MENUSUB SELECTION

FACTORY
ADJUSTMENTACTION
TIMEMEASUREMENT START TIME to change

measure-start time.
A-27
Defect of Hemolyzer volume. Touch
SELECT
MENUSUB
FUCTORY
SELECTION
ADJUSTMENTSYRINGE
VOLUMEHEMOLYZER
SYRINGE
to
Hemolyzer syringe volume.

A-28
Fluid leakage of Hgb block.
Remove HGB block from the unit for

disasemblying cleaning.
Wipe liquid around HGB block of WBC cell,
61
change
then refill it with silicone.

A-29
Fluid
leakage
of
Suction Remove probe cleaning part from the unit
probe.
for disasemblying cleaning.

Position of probe cleaning part (Ref. A-14)
Defect of connection cable (Ref. A-47)
A-30
Fluid leakage of Cell.
Check and see if any cracks on Cell.

A-31
Defect of Sample.
Check and see if it has become solid due to

insufficient aggitation right after sampling.
Stir well before measurement, re-measure.
Check if 12 hours+ have passed after sample
A-32
Mis-suction of sample.
Be sure to put the tip of suction probe into

sample then take suction.
In case of less quantity of sample, sample
blood agian.
CAUSES
A-33
CORRECTIVE ACTION
Defect of Sample syringe Measure Control blood, then touch

volume.
SELECT MENUSUB SELECTION FUCTORY

ADJUSTMENTSYRINGE
VOLUMESAMPLE
SYRINGE to change volume.

Defect of sample syringe motion (Ref. A-35)
A-34
Defect of Diluent syringe Touch SELECT MENUMAINTENANCE LIQUID

motion.
CIRCUIT SYRYNGE ACTION

to confirm Diluent syringe motion.
Adjust fitting of gears at Drive unit.
Adjust so that syringe part travels smoothly.
Check packings and change if worn and torn.
Check and see if sheild plate is surely
62
sensored. If not, adjust it.

Check sensor. If broken, replace with new one
A-35
Defect of Sample syringe Touch SELECT MENUMAINTENANCE

motion.
LIQUID CIRCUITSYRINGE ACTION

to confirm Sample syringe motion.
A-36
Defect of Hemolyzer syringe Touch SELECT MENUMAINTENANCE

motion.
LIQUID CIRCUITSYRINGE ACTION

to confirm Hemolyzer syringe motion.
63
CAUSES
A-37
CORRECTIVE ACTION
Defect of Pump/ solenoid Remove Pump/solenoid valve for cleaning.

valve.
Give +/- pressure to solenoid valve with

syringe tube to check leakage. If leaking,
replace with new solenoid valve.
A-38
Defect
of
Suction
probe Check and see if suction probe is bent. If so,
motion.
replace with new suction probe.

Check and see if sheild plate in probe travel
part is surely sensored. If not, adjust it.
Check pully timing belt, motor bearing. If worn
and torn, replace with new ones.
In case suction probe is stuck, connect
syringe tube with probe and give +/- pressure
Replace with new suction probe.
A-39
Bent/clogging
of
wiring Check bent/clogging of wiring tubes. If any
tubes.
clogging found, clean and remove.

In case bent part is found, change new one.
A-40
Leakage of wiring tubes.
In case holes/cracks are found on wiring tube,

replace with new ones.
In case joint part of tube and tube joint is
loose, replace with new tube.
A-41
Loose tube joint.
Confirm that tube joint of Solenoid valve and

Tank etc. has been firmly tightened.
In case O-ring of joint part is worn and torn,
replace with new one.
A-42
Wrong piping.
Compare the piping with the whole piping

diagram and correct.
Compare input/output of solenoid valve pump
with the whole piping diagram and correct.
A-43
Air leakage of
part.
Detection Check fixing bolts at Detection part. In case

they are loose, screw them again.
64
Check and see if any cracks at Detection part.

In case they are found, replace Detection part.
In case packing for Electrode is worn and
torn, replace with new one.
In case O-ring of joint part is worn and torn,
replace with new one
A-44
Air leakage of Waste/Supply Check and see if any bad sealing of Water
tank.
Supply/Waste tank. If found, fix it with silicone

and screw bottles tight.
A-45
Influence by inner noise.
Correct loose fixing of sheild plate inside unit

Give washer to bolts and fix them tight.
A-46
Influence by outer noise.
Put the unit away from noise generation area.

Use single receptacle and use Ground earth.
A-47
Defect of connection cable.
Check and see if any damages on cables.

If any found, replace with new ones.
A-48
Wrong wiring.
Compare the wiring with the whole wiring

diagram and correct.
Comfirm that silk and cable NO. of the board
is not wrong.
8-3 Troubles in operation

SYMPTOMS
No power to instrument
Uncontrolled CPU
CAUSES
Ref
Power cord is unplugged
B-1
Instrument fuse is blown
B-2
Wrong wiring at power SW board
B-3
Power switch is broken
B-4
Lowering of power voltage
B-5
A-47
Wrong wiring
A-48
Lowering of power voltage
B-5
CPU board is broken
B-6
Lowering of 5V voltage
B-7
A-45
A-46
65
Improper key operation
Improper printer work
A-47
Wrong wiring
A-48
Switch is broken
B-8
Touch panel LCD is brokn
B-9
A-45
A-46
A-47
Wrong wiring
A-48
Improper print paper
B-10
Printer I/F board is broken
B-11
Clogging in printer head
B-12
Printer lever is in upper position
B-13
A-47
Wrong wiring
A-48

CAUSES
B-1
Power cord is
CORRECTIVE ACTIONS
Turn the power to the unit is off, then plug in.
unplugged
B-2
B-3
Instrument fuse is
Remove blown fuse and replace with new
blown
fuse(5A).
Wrong wiring at
Check wiring at Power switch board.
Power SW board
66
B-4
B-5
Power switch is
Turn on and off the switch several times.
broken
Change new power switch.
Lowering of power
Confirm that power voltage is AC100V.
voltage
B-6
CPU board is
Check and see if any damaged solder part on
broekn
CPU board.
Change new CPU board.
B-7
Lowering of 5V
Change new switching regulator.
voltage
B-8
Switch is broken
Check and see if switch has been in pressed

position all the time.
Change new switch.
Change new switch board.
B-9
B-10
B-11
Touch panel LCD
Change new Touch panel LCD.
is broken
Change new LCD board.
Improper print
Set print paper inside/outside correctly.
paper
Change new print paper.
Printer I/F board
Change new Printer I/F board.
is broken
B-12
Clogging in printer
Remove clogged matter.
head
Touch SELECT MENUMAINTENANCE OUTPUT

TESTBUILT-IN PRINTER for test printing to
confirm printing motion.
B-13
Printer lever is in
Lower head-up printer lever.
upper position
Touch SELECT MENUMAINTENANCE OUTPUT

TESTBUILT-IN PRINTER for
test printing to confirm printing motion.
67
9 REFERENCE
9-1 Mode Switch
Following is the explanation of Mode Switch located in the back of instrument.
No.
1
FUNCTION
ON
OFF
Mode
Maintenance Mode
Normal Mode
Language
English
Japanese
EXT. Printer
Not in use
Not in use
Not in use
Not is use
Not in use
EPSON
HP
1. Changing Mode
The following functions are added by Maintenance Mode.
SUB SELECTION is displayed without any special operation.
Automatic water supply is not operated when power is turned ON.
Threshold and peak of WBC histogram are printed if SAMPLE No. is set in
advance.
Exact value of background count of blank measurement is displayed.
After finishing maintenance, make sure to retrun the mode switch OFF position
and then turn the power OFF and ON again.
2. Language
Normally place the switch ON position.
3. Not in use
4. External Printer
Select the external printer you want to use. This mode is active with ROM
Ver. 2.36/5.02 or newer. If the ROM version is older than 2.36/5.02, only EPSON printer
Is usable.
EPSON
Control Code ESC/P
Operation checked by LQ-300

LX-300+ (ROM Ver. 5.13 or newer)
HP
Control Code HP PCL
Operation checked by hp deskjet 5650
Turn the power OFF and ON again after changing mode switch position.
68
5. Not in use
6. Not in use
7. Not in use
8. Not in use
9-2 Note for using external printer
The first printing right after connecting an external printer to PCE-210, some characters may be
missing or unnecessary characters may be printed on printing contents in the test printing or at
the end of measurement. To avoid the situation, please follow the undermentioned:
1. Make sure that the printers power is off when you connect a printer cable.
2. Make sure that you turn on PCE-210s power first, then the printers power, at any time.
3. When you turn off the power, be sure that you turn off the printer, then PCE-210.
4. DO NOT turn on and off the power of PCE-210 while the printers power is ON.
5. Please also set a printer paper in the internal printer.
6. In case printer errors (running out of paper, opening the cover, etc.) happen, you can not
do the recovery operation by PCE-210, unlike the case you connect PCE-210 to PC.
EPSON
HP
69
9-3 RS-232C Output

Output format
Item Name
Output Order
Output digits
STX
STX
Sample No.
5 digits
00000
Date
10 digits
yymmddhhmm
WBC
5 digits
000.0
LY
5 digits
000.0
MO
5 digits
000.0
GR
5 digits
000.0
LY%
5 digits
000.0
MO%
5 digits
000.0
10
GR%
5 digits
000.0
11
RBC
5 digits
00.00
12
Hgb
5 digits
000.0
13
HCT
5 digits
000.0
14
MCV
5 digits
000.0
15
MCH
5 digits
000.0
16
MCHC
5 digits
000.0
17
RDW
5 digits
000.0
18
PLT
4 digits
0000
19
PCT
5 digits
0.000
20
MPV
5 digits
000.0
21
PDW
5 digits
000.0
22
WBC Histogram
3 digits X 200 data
000 X 200
23
RBC Histogram
3 digits X 200 data
000 X 200
24
PLT Histogram
3 digits X 200 data
000 X 200
25
ETX
ETX
Forwarding Speed :9,600 bps

Start bit
Output code
:1 bit
70
Data bit
:8 bit ASCII Code
Parity bit
: None
Stop bit
: 1 bit
Connecting cable
D-SUB9 Cable
PCE-210
External
Device
TxD 2
2 RxD
RxD 3
3 TxD
5 GND
GND 7
Format Example
Data
Data transfer
STX
02
No.
20
20
20
20
32
YY
00
30
30
MM
04
30
34
DD
01
30
31
hh
00
30
30
mm
15
31
35
WBC
14.2
20
31
34
2E
32
LY
11.1
20
31
31
2E
31
MO
1.3
20
20
31
2E
33
GR
1.8
20
20
31
2E
38
LY%
78.2
20
37
38
2E
32
MO%
9.2
20
20
39
2E
32
GR%
12.7
20
31
32
2E
37
71
RBC
4.43
20
34
2E
34
33
Hgb
15.1
20
31
35
2E
31
HCT
47.9
20
34
37
2E
39
MCV
108.1
31
30
38
2E
31
MCH
34.0
20
33
34
2E
30
MCHC
31.5
20
33
31
2E
35
RDW
15.5
20
31
35
2E
35
PLT
289
20
32
38
39
PCT
0.280
30
2E
32
38
30
MPV
9.6
20
20
39
2E
36
PDW
20.2
20
32
30
2E
32
WBC
20
20
30
100
31
30
30
20
20
30
RBC
20
20
30
100
31
30
30
20
20
30
PLT
20
20
30
100
31
30
30
20
20
30
Histogra
Histogra
Histogra
m
ETX
03
72
3 digits*200data
3 digits*200data
3 digits*200data
9-4 ROM Replacement

The following is how to replace ROM when the manufacturer asks to do so.
1. Before replacing the ROM, each parameter of present setting has to be stored. Please
save the necessary stored sample data because all of them will be deleted by changing
ROM.
2. Press PRINT switch on the SUB SELECTION so that the parameters will be printed out
from the built-in printer.
3. Set the DIP switch # 1 of the back side of equipment ON, then turn off the power and
pull out the power supply code. Replace the ROM.
4. Turn the power on. Measurement stand-by screen will be displayed without a water
supply cycle.
5. Go to SUB SELECTION BASICAL SETTING INITIALIZATION to initialize the
equipment. At this time, SUB SELECT MENU can be displayed without any special
operation.
6. Now each parameter returned to the initial value, so please input the value again. After
finishing re-input, turn DIP switch # 1 OFF and turn off the power.
9-5 Clog Detection

Clog Detection Method 1
The following calculation and detection is carried out in software when RBC value is more
than 500,000 and Hgb value is more than 3.0 mg/dl.
1. Total RBC value is obtained.
2. 70% value of total RBC is calculated
3. The value obtained in above No. 2 is divided by RBC sample aspiration volume.
4. When total measured value for 1 second is smaller than the value obtained in above No.
3, PCE-210 detects it as RBC clog.
The following calculation and detection is carried out in software when WBC value is more
than 1,000 and Hgb value is 3.0 mg/dl.
73
1. Total WBC value is obtained.

2. 70% value of total WBC is calculated.
3. The value obtained in above No. 2 is divided by WBC sample aspiration volume.
4. When total measured value for 1 second is smaller than the value obtained in above No.
3, PCE-210 detects it as WBC clog.
Clog Detection Method 2

Number of the blood cell pulse is counted in each cycle fixed during measurement and PCE210 makes comparison in the number of pulses for each two adjoining cycles. If there is more
than 30% decrease detected in number of pulses, for example, if the previous cycle has shown
100 pulses detected and the following cycle shows 70 pulses only, PCE-210 concludes that
there is abnormality in the detection (measurement) part, showing messages such as RBC
CLOG, WBC CLOG and etc. However, those error messages are displayed only after the
measurement cycle is completed. Even if the abnormality is detected in the middle of the
measurement, counting itself is continued until the end of the measurement cycle. Users are
also able to see * in the left side of parameter names both in the display and in print-outs
when there was abnormality, such as clogging.
100%
70%
9-6 Measurement Control for Pressure Volumetric Method
Fixed volume measurement by pressure control
A negative pressure pump of PCE-210 makes aspiration pressure to the fixed value of
150mmHg just before aspirating diluted sample. This fixed pressure is kept and controlled all
the time through measurement operation by pressure sensor. Under the circumstance of the
150mmHg pressure, a valve for sample aspiration is opened for the measurement. The
74
aspiration time is set in advance as a factory adjustment and never varies in each individual
measurement. Therefore, volume of the diluted sample can be fixed same for each
measurement due to the aspiration made under condition of fixed pressure for the fixed time.
In case there is variation seen in pressure during measurement, PCE-210 detects its
abnormality in aspiration flow and stops measurement.
75

Pce 210sme 503

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PCE-210SME Ver. 5.

FULL AUTOMATIC BLOOD CELL COUNTER

For safe use of PCE-210, please read this Installation Instruction

PCE-210SME Ver. 5.03

FULL AUTOMATIC BLOOD CELL COUNTER

2 COMPONENTS & FUNCTION OF THE UNIT

2-3RIGHT SIDE VIEW

2-4HEMOLYSYS REAGENT HOLDING SPACE

2-5MAINTENANCE SPACE OF LIQUID CIRCUIT

3-1OPENING CARTON BOXES

3-4-1Connecting power code

3-4-3Setting print paper

3-6-3Filling of cleaning solution

4-2THE WHOLE WIRING DIAGRAM

4-3LIST OF ELECTRIC CIRCUIT BOARDS

PCE-210SME Ver. 5.03

4-4-2 Pressure sensor board

4-4-3Printer I/F board

4-4-4Panel switch board

4-4-9Power switch board

4-4-11WBC detection board

4-4-12RBC detection board

4-4-13+5V/+12V switching regulator

5-1THE WHOLE PIPING DIAGRAM

5-2LIST OF ELECTRIC PARTS FOR LIQUID CIRCUIT

5-3FUNCTION OF LIQUID CIRCUIT

6-1 FACTORY ADJUSTMENT

6-1-1 SYRINGE VOLUME

6-1-2 SUCTION VOLUME

6-1-3 FUCTORY CALIBLATION

6-1-4 ACTION TIME

6-1-5 MOVEMENT ADJUST

6-1-6 DISC. VOLUME

6-2 FACTORY CONFIRMATION

6-2-1 Hgb UNIT

6-2-2 SUCTION PRESSURE

6-2-3 WORKING TIMES

6-3 BASICAL SETTING

6-3-1 DISCRIMINATION OUTPUT

6-3-2 CLEANING SOLUTION BOTTLE

PCE-210SME Ver. 5.03

7-1MAINTENANCE FOR LONG TIME STORAGE

7-3 CLEANING DETECTOR

8-1 ERROR MESSAGE

8-2 TROUBLES IN MEASUREMENT

8-2-1 Problems and causes

8-2-2 Causes and corrective action

8-3 TROUBLES IN OPERATION

8-3-1 Problems and causes

8-3-2 Causes and corrective action

9-1 MODE SWITCH

9-2 NOTE FOR USING EXTERNAL PRINTER

9-3 RS-232C OUTPUT

9-4 ROM REPLACEMENT

9-5 CLOG DETECTION

9-6 MEASUREMENT CONTROL FOR PRESSURE VOLUMETRIC METHOD

PCE-210SME Ver. 5.03

1 SAFETY NOTES TO USERS

If treated mistakenly, a danger to cause death or wound is

If treated mistakenly, a possibility to cause death or wound

If treated mistakenly, a danger to cause would and physical

damage is expected to happen.

Necessary Action before using