Escolar Documentos
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FOR THE
IDENTIFICATION OF
BACTERIA
PRESENTED BY: NEETU SING
(NITRATE
MODERATED BY: Dr. Shinu P
REDUCTION )
Department of Microbiology
INTRODUCTION
Biochemical
of the organisms
Bacteria
produces
NITRATE REDUCTION
TEST
Peptone
5g
Distilled water
1litre
REAGENT - A
Sulphanilic acid
8g
Acetic acid(5N),30%
1lit
PRINCIPLE
nitrate reductase
NO3 + 2e +2H
NO2 + H2O
diazonium salt
IF NO COLOR
TRUE NEGATIVE
FALSE NEGATIVE
( NO3)/
(NH3,N2,NO,N2O and
hydroxylamine )
Zinc Dust
NO3 to NO2
Red colour
True Negative
reaction
PROCEDURE
Inoculate nitrate broth(5ml) with
organism and incubate at 37C , 18-24
hours
RESUL
TS
enterocolitica biovar
Vibrionacae except- Vibrio melschnikovii
Pseudomonacae except- Pseudomonas
diminuta
Pseu. paucimobilis
Alkaliginacae except-Alkaligens fecalis
Pasteurella canis