This tests aim was to show how toxic an ecosystem is by measuring how the

bioluminescence generated from Aliivibrio fischeri bacteria is affected by the presence

of chemical substances in an environment.
The measurement can be applied to groundwater, surface water, marine water, liquid
phases, solid phases and other ecosystems as well. Its important to say that this test is a
single species test, so it wont give the entire information about the ecosystem if it
involve more species
By using a cell suspension, grow solutions and serial dilutions, we were measuring
the intensity of luminescence with different metal concentrations. This way we can
evaluate how the chemicals affect the luminescence, implying in how toxic the solution
is.
The initial data was:
Sample
NaCl
NaCl
Cu 20 ppm
Cu 40 ppm
Cu 80 ppm
Cu 120 ppm
Cu 160 ppm
Cu 200 ppm
Cu 400 ppm
TiO2 0,005 ppm
TiO2 0,05 ppm
TiO2 0,5 ppm
TiO2 5 ppm
TiO2 50 ppm
TiO2 500 ppm
NaCl
NaCl

I0
652081
653002
651035
651771
650907
650534
650340
650118
650211
649879
650411
650082
650524
649861
650088
649715
651123

I30
651880
652987
651860
651615
651407
640825
636074
559081
1567
648850
635348
608953
439831
99847
81216
649739
651324

Ical

H%

With the method shown in manuscript, the f, Ical and H% obtained by the equations
below are present in the next chart:
=

30
0
= 0

% = 100

30

Sample
NaCl
NaCl
Cu 20 ppm
Cu 40 ppm
Cu 80 ppm
Cu 120 ppm
Cu 160 ppm
Cu 200 ppm
Cu 400 ppm
TiO2 0,005 ppm
TiO2 0,05 ppm
TiO2 0,5 ppm
TiO2 5 ppm
TiO2 50 ppm
TiO2 500 ppm
NaCl
NaCl

I0
652081
653002
651035
651771
650907
650534
650340
650118
650211
649879
650411
650082
650524
649861
650088
649715
651123

I30
651880
652987
651860
651615
651407
640825
636074
559081
1567
648850
635348
608953
439831
99847
81216
649739
651324

Ical

H%

1,000
1,000
651035
651771
650907
650534
650340
650118
650211
649879
650411
650082
650524
649861
650088

-0,1267
0,02393
-0,0768
1,49247
2,19362
14,0032
99,759
0,15834
2,31592
6,32674
32,3882
84,6356
87,5069

1,000
1,000

With the results, two diagrams were made:

H% x [Cu]
120
100

99.759

80

H%

60
40

0.02393

20

-0.1267

0
0
-20

50

-0.0768
14.0032
1.49247
2.19362
100

150

200

250

[Cu] in ppm

300

350

400

450

H%

H% x [TiO2]
100
90
80
70
60
50
40
30
20
10
0
0.005

0.05

0.5

50

500

[TiO2] in ppm

Analyzing the results its possible to conclude that the Titanium Dioxide is more
toxic than Cu, to these bacteria. It is explained by the fact that with 0,05 ppm of TiO2
the luminescence was more affected than with 120 ppm of Cu.
It can be conclude too that the bacteria has a certain limit of tolerance to these
substances. After 200 ppm of Cu, the luminescence dropped exponentially. The same
effect was observed after 0,5 ppm of Titanium dioxide. The diagram stops its linear
behavior and gets an exponential behavior. It was observed too that you need to add a
certain amount of concentration to the start the prejudicial effect.
-

For colony-counting practice, the result is the following table:

Dilution
Colonies
(Meat). 1000
91
(Meat). 10000
10
Endo agar
19