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INTRODUCTION
Spectrophotometry is a method to measure how much a chemical substance
absorbs light by measuring the intensity of light as a beam of light passes
through sample solution. The basic principle is that each compound absorbs or
transmits light over a certain range of wavelength. This measurement can also
be used to measure the amount of a known chemical substance. The
quantitative study utilizes the device called spectrophotometer. The device has
two main parts, the spectrometer that emits a specific light wavelength and the
photometer that measures the light intensity which the sample transmitted.
The main goal of experiment is to study equilibrium constant of reaction through
analysis by spectrophotometry. The experiment intends to familiarize students
with the spectrophotometer and with a new method of obtaining the equilibrium
constant of a reaction. The reaction between
iron and thiocyanite is utilized in this experiment. It has the reaction system,
Fe3+
(aq)
In this reaction system, blood red complex [Fe(H 20)5(SCN)]2+ is formed. The
absorbance of this complex will be measured by the UV-Vis spectrophotometer
and the concentration can then be obtained through the use of the BeerLamberts Law. It relates the light absorption of the colored substance in the
system to its concentration. It has the equation,
A = bc
Where,
A= absorbance
= molar absorptivity coefficient in M-1 cm-1
b = path length
c = analyte molar concentration
With the use of the calculated concentration, the equilibrium constant can be
computed using the expression,
Keq = [FeSCN2+]/ [Fe3+] [SCN-]
The method can only be executed accurately if the solutions used are highly
diluted and the reagents have a high purity or else the data will not be in a
regressing pattern. In addition to that, the experiment must be performed with
precisely same temperature for all solutions since K eq changes with temperature.
RESULTS AND DISCUSSION
After the calibration of the spectrophotometer, the unknown solutions are tested.
Another blank solution was prepared and used for this part of the experiment to
recalibrate the base of zero of the apparatus since the amount and the
concentration of SCN- used in this part differs from that of the standard solutions
even if the same cuvette were used. In this part of experiment, more diluted
concentration of SCN- and Fe3+ is used. This is to isolate the metal complex
[FeSCN2+]. It is possible since in diluted form, SCN - and Fe3+ have negligible
absorbance. In addition, this type of spectrometry can only yield results if the
solutions to be analyzed are highly diluted and come from reagents with high
purity. Through algebraic manipulation of the the equation of the best fit curve,
Y= 2865.4x 0.0051
The equilibrium concentration of the [FeSCN2+] will be obtained. Listed in the
table below is the calculated [FeSCN2+]eq with the actual absorbance of unknown
solutions .
Table 3. Equilibrium Concentration of [FeSCN2+]
Unknow
Abs
[FeSCN2+]eq
n
Solution
1
0.341
1.17 x 10-4
2
0.471
1.62 x 10-4
3
0.570
1.97 x 10-4
Also through calculations, the initial concentration of Fe 3+ and SCN- can be
obtained. The acquired values for the unknown solutions are listed in the table
below.
Table 4. Initial Concentrations of Fe3+ and SCNSoln
[Fe3+]i
[SCN-]i
1
2
3
6.0x10-4
8.0x10-4
1.0x10-3
1.0x10-3
1.0x10-3
1.0x10-3
Determinat
e/
Indetermin
ate
Indeterminat
e
Systematic/
Random/Gr
oss
Random
Determinate
Systematic
Determinate
Systematic
Determinate
Systematic
Determinate
Systematic