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Note: AS core practicals may also be included e.g. May 2010 paper
had beetroot experiment
Question 2
This second question is a data based question.
Students may be required to suggest a suitable hypothesis/null
hypothesis (read revision guide page 88)
The student should be able to tabulate data ( page 87)
Calculate means
Present the data in suitable graphical form (page 87)
Apply a statistical test ( notes below & page 88)
The student should be able to explain the meaning of any
calculated test statistic in terms of 5% significance limits.
5% significance level which is also written as p= 0.05means
that there are 5 chances in 100 that the result obtained in an
investigation could occur even if there was no difference
between two data sets
All biological investigations are based on 5% significance
levels or 95% confidence level
Types of statistical tests
You are not expected to know the formulae or fine details of each
test but you should concentrate on selecting the correct type of
test and demonstrate your understanding of how to interpret the
results
There are three main statistical tests
1.
2.
3.
4.
Students T test
Mann- Whitney U test
Spearmans Rank Correlation test
Chi-squared test
1. Students T test
This is used to measure the amount of overlap between two
data sets.
It tests whether there is significance difference in means between
two data sets e.g. the difference between the mean lengths of
leaves in a shaded site and a sun exposed site.
The calculated t value is compared against the critical value at
5% significance level for the number of degrees of freedom (d.f.). d.f
= na+nb-2 where n is the number of samples
If the calculated t value is larger than the critical value, we
reject the null hypothesis and accept the alternate hypothesis
that there is a significant difference between the means of the two
data sets.
Example 1
A t test was applied to test whether there is a significance difference
between mean of red blood cells of athletes before and after
training in a highland.
ANSWER
Example 2
Answer
Answer
6
4. CHI-SQUARED TEST (X)
- It is used to test for goodness of fit, that is, how the observed
and expected values compare i.e. one must have expected
values so that during the investigation the observed values are
compared with expected values.
- The requirements for the X test are:
The chi-squared value which is always given
Degree of freedom (n-1)
Confidence level = 95%
Critical value which is obtained from the table using
significance level (5%) and degree of freedom (DF).
If the X value is more than or equal to critical value,
reject the null hypothesis and vice versa.
(b)
This part of the question requires the student to give a
detailed method and explanations of how important variables
are to be controlled.
- A clear statement of the dependent variable is given i.e.
exactly what is to be measured.
- A clear statement of the independent variable is stated
- The range of the independent variables is suggested.
- A clear consideration of the time over which the
investigation is to be carried out.
- Suitable details of how the measurements are taken or
how data is collected.
- At least two variables that can affect the investigation
are identified.
- A description of how the above to variables are
controlled.
- The investigation must be repeated for reliability.
- The total mark for the question is 10 which includes 2
marks for SPG (Spelling and Grammar)
(c)
This part of the question requires the student to give a
clear explanation of how the data are to be recorded,
presented and analyzed in order to draw conclusions.
- The student is expected to draw a table of results which
matches the proposed method.
- The tables should have clear headings and units.
- The means should be calculated from the repeated data.
- A suitable graph, a line or a bar graph, should be drawn
with correctly labeled axes.
- The student should make reference to a suitable statistical
test e.g. a t-test or a Mann-Whitney for significant
difference, a Spearmans Rank test for correlations, or Chi
test goodness of fit.
(e) This question requires the student to give limitations for
method of investigation used.
- Limitations are genuine difficulties or factors that the
investigator cannot be able to control.
Examples:
- If you are comparing two areas in ecology, its difficult to
match the conditions on both sites.
- It is difficult to control environmental conditions
Good luck.