Experiment No: 17

AIM:To identify the different kind of foodstuff in the given samples.

Experiment

Observation

Inference.

TEST FOR CARBOHYDRATES:

Molischs test :To the sample A in test Red rings appear at the
Sample A contains
tube add little water and Molischs
junction of the two layers. carbohydrates.
Reagent(10% solution of alpha naphthol
in ethanol).Slowly pour conc sulphuric
acid by the sides of the test tube
Fehlings Solution test: To the sample No yellow or red
A in test tube add little water & add
precipitate is observed
Fehlings solution(prepared by mixing
equal volumes of Fehlings solution A &
solution B). Heat it gently on boiling
water bath.

Reducing sugar absent

in sample A.

Iodine test: To the sample in test tube A blue color is observed

add little water, and then add 2-3 drops
of iodine solution.

Sample A contains
starch

TEST FOR PROTEIN

Biuret test: To the sample B in test tube Bluish violet colouration Sample B contains
add little water, and then add few drops seen.
proteins.
of NaOH solution & few drops of
1%CuSO4 solution.
Xanthoprotein test: To the sample in
Yellow precipitate is
test tube B add little water, then add few observed.
drops of conc.HNO3.(heat)

Sample B contains
proteins.

TEST FOR FAT

Acrolein test: To the sample C in test
tube add few crystals of potassium
hydrogen sulphate( KHSO4). Heat the
test tube gently then strongly

A pungent irritating odour Sample C contains oils

due to the formation of
and fats.
acrolein is observed

Translucent spot test: Press a little of

sample C in the folds of filter paper.

On unfolding the filter

paper, the appearance of

Sample C contains
oils and fats.

translucent or greasy spot

is observed. Spot grows
larger on heating and
drying the filter paper.

Result :
From the above test we identify that
Test tube A contains Starch
Test tube B contains Proteins
Test tube C contains Oils & Fats