KATA PENGANTAR

ABSRAK
ABSTRACT
DAFTAR ISI
DAFTAR LAMPIRAN
DAFTAR GAMBAR
DAFTAR TABEL
I.
PENDAHULUAN
II.
TINJAUAN PUSTAKA
II.1.
Lichen
II.1.1. Morfologi
II.1.2. Stereocaulon
II.1.3. Stereocaulon halei Lamb.
II.1.4. Potensi dan pemanfaatan lichen
II.2.
Asam urat dan hiperurisemia
II.3.
Xantin oksidase
II.4.
II.5.
II.6.

Allopurinol
Uji penghambatan aktivitas enzim xantin oksidase
Uji kinetika penghambatan aktivitas enzim xantin

oksidase
III.
METODE PENELITIAN
III.1.
Tempat dan waktu penelitian
III.2.
Alat dan bahan
III.2.1. Alat
III.2.2. Bahan
III.3.
Prosedur penelitian
III.3.1. Penyiapan sampel uji
III.3.1.1. Pengambilan sampel
III.3.1.2. Ekstraksi dan skrininig fitokimia
III.3.1.3. Isolai senyawa utama lichen S. Halei Lamb.
III.3.2. Pengujian aktivitas enzim xantin oksidase
III.3.2.1. Pembuatan reagen
III.3.2.2. Pembuatan larutan uji
III.3.2.3. Prosedur pengujian aktivitas xantin oksidase
3.3.3. Perhitungan nilai % inhibisi dan IC50 aktivitas enzim
xantin oksidase
3.3.4. Penentuan kinetika penghambatan enzim
IV.
HASIL DAN PEMBAHASAN
IV.1.
Hasil
IV.2.
Pembahasan
IV.2.1. Penyiapan sampel uji
IV.2.2. Pengujian aktivitas enzim xantin oksidase
IV.2.3. Penentuan kinetika penghambatan

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enzim
V.
KESIMPULAN DAN SARAN
V.1.
Kesimpulan
V.2.
Saran
DAFTAR PUSTAKA
LAMPIRAN

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A research on developing anti-gout drug throught examine xanthine oxidase

activity of the extracts and isolates of lichen Stereocaulon halei Lamb. have been
conducted. Extract of lichen S. halei which yield by gradient solvent extraction
from hexan, ethyl acetate, aceton, methanol and also isolate atranorin, lobaric acid
and methyl orcinol carboxylate (MOC) were examined that activity, allopurinol
was used as comparison control. The activity of enzym was examine continuous
spectrophotometric rate method using microplate 96-well and the absorbances
were measured using a microplate reader (BioRad Xmark). Extract of ethyl
acetate provided the strongest inhibitory activity against the enzyme xanthine
oxidase compared to other extracts with 81% inhibition at a concentration of 250
ug/mL. While the isolates, lobaric acid provides the strongest inhibition activity
compared to all the isolates against xanthine oxidase activity by 0,7% inhibition at
a concentration of 10 ug/mL. Inhibition of xanthine oxidase activity from extracts
and isolates lichen S. halei were smaller than allopurinol at a concentration of 10
ug/mL which provides 99% inhibition of the xanthine oxidase activity. Examine
on the kinetics of ethyl acetate extract and lobaric acid showed both have
inhibitory mechanism uncompetitif to the substrate (xanthine).