Ideal set up

of Health
Facility for TB
Microscopy
Service

MICROSCOPY CENTER
out door sputum
collection area

Collection Area for Sputum Sample

A designated area
wherein PRIVACY of
PATIENT is
considered
- Away from patient
waiting area or
passer-by
- Well- ventilated
- Not totally enclosed
- With shade (plastic
roofing is preferred;
natural sterilization)
to protect from rain.
CHD-Central Luzon

Collection Area for Sputum Sample

- sink

with
running water
(preferred) for
hand washing
- presence of
soap for good
hygiene
- poster/ hand
washing
instruction

Collection Area for Sputum Sample

- with

signage
Collection Area

Improper set-up
- without

enough
shade and
located
beside the
air-con
exhaust

MICROSCOPY CENTER
LABORATORY SET-UP
for AFB microscopy

LABORATORY SET-UP
LABORATORY ROOM AREA
should be:
1. well-ventilated
2. enough space at least 4 x 4 sq.meter
for laboratory microscopy work
3. continuous supply of electricity and,
4. tap water must be provided as well

Set-up of a microscopy laboratory

and Quality Assurance Center:

LABORATORY ROOM ARRANGEMENT:

Ideally, a TB microscopy center should at least
consist of the following sections:

1)
2)
3)
4)
5)
6)
7)

receiving area
smearing area
staining area
microscopy area
writing area
releasing area
storage space

LABORATORY ROOM SECTIONS

and its FUNCTIONS
1.

RECEPTION area

for receiving the Specimen

2.

SMEAR PREPARATION
area

for smear preparation and heat

fixed smear

( well ventillated or with nearby

installed exhaust fan )

3.

STAINING area

for staining smears

4.

MICROSCOPY area

for reading of smears

5.

WRITING area

for results and recording data

6.

STORAGE SPACE

for stain reagent & other lab

supplies

7.

RELEASING area

for the releasing results

RECEPTION AREA
1. NTP Laboratory
Register
2. Ballpen
3. Sputum cup
4. Gum label

Smear Preparation Area:

1.

Ordinary pen or pencil for

labeling

2.

Alcohol lamp

3.

Frosted glass slides

4.

Slide rack

5.

Wet spirit cotton

6.

Washing bottle

STAINING AREA:
1. AFB stain
reagents
2. Staining bridge
3. Forcep
4. Lighter

Sink for Staining AFB Smears

- deep

sink is preferred (at least 1 foot deep)

- dark

colored tile is preferred than stainless

(easily corrodes)

The Materials & equipment in

AFB microscopy area:
1.Electric Binocular microscope
2.Oil immersion (Cargill Type A)
3.2 Koplin jars with xylene
4.Mappe
5.Lens paper
6.Dry cotton
7.Ballpen-black and red color
8.Slide box

Microscopy Section

SAFETY STORAGE SPACE:

1. AFB reagents
2. Sputum cups
3. NTP Forms
*NTP laboratory register
*Lab request form
*NTP quarterly report form,
and
4. Other NTP laboratory
supplies

RECORDING AREA & RELEASING

OF RESULTS:
1. Laboratory request form
2. NTP laboratory register
3. NTP quarterly report forms
and ballpen

LABORATORY SET-UP
An ideal laboratory set-up that is
clean, dust-free, well-organized
and well-equipped one creates
convenient working
environment
As well as facilitates processing of
AFB Smear Microscopy
Examination easily and accurately

Set-up of a microscopy laboratory

and Quality Assurance Center: