Appendix

CHAPTER 1 – TRANSPORT IN PLANT

Aim/ : To study the function of xylem tissues in flowering plant. √ E1 PI 1

Objective

Problem : Do xylem tissues transport water ? √ E1 PI 2

Hypothesis : Xylem tissues transport water . √ E1 PI 3

Variables
Manipulated : Types of vascular tissues , xylem tissues and phloem
tissues

Responding : Red stain on the xylem tissues √ E1 PI 4

Fixed : Types of plant / balsam plant // types of staining / eosin

Material : Eosin , balsam plant , distill water

Apparatus : White tile , conical flask , pen-knife ( scalpel ), glass √ E1 PI 5

slide , cover slip , microscope , forceps

Technique : Observing and identifying the presence of red stain on √ E1 PI 6

the xylem tissues

Procedure : 1. Roots of the balsam plant are washed under

running tap water to get rid of the soil.

2. The balsam plant is then placed in a conical

flask containing 200 ml of eosin.

3. The balsam plant is placed under bright situation

for 30 minutes. After 30 minutes , the balsam
plant is removed from the conical flask , then the
roots are washed under running water to get rid
of the excess eosin.

4. Two parts of the plant, the roots and the stem √ E1 PI 7

are observed.

5. A pen-knife ( scalpel ) is used to cut a thin cross

section of the roots.

6. The section is mounted in a drop of water on a

microscopic slide and is covered with a cover
slip
 7. The section is examined under a microscope
using low power objectives lens.

8. Diagram of the cross section of the roots is

drawn.

9. The structures that are stained red with eosin

are shaded and labeled.

10. Steps 6-9 are repeated for the stem.

TEACHER’S
SIGNATURE

Result : The Cross Section Root And Stem Of

A Balsam Plant

( a ) Root
√ E3 PI 1

√ E4 PI 1

(b) Stem

Magnification: 10 x 40
Discussion :
1. Xylem tissues in the root and stem are stained √ E4 PI 2
red with eosin.
2. Phloem tissues in the root and stem are not
stained red with eosin.
3. Xylem tissues transport water and phloem
tissues does not transport water.

Conclusion : Xylem tissues transport water √ E4 PI 3

Hypothesis is accepted