BACTERIAL ADHESIN IN Staphylococcus aureus

T.PRADEEP
 Fibronectin binding protein(FnBPA)
• FnBPA mediates internalization of S. aureus
into endothelial cells & promotes rapid
aggregation of human platelets
• FnBPA have high affinity binding repeats
(FnBRs) for fibronectin
• The cell wall anchoring region are all C
terminal region
• There are 11 FnBRs in A domain in which 6
has high affinity to N – terminal domain of Fn
2F1-4FI OF FIBRONECTIN
3F1-5F1 OF FIBRONECTIN
 Intrinsic disorder has been exploited in 2
different ways

•   It allows the formation of high affinity

interaction of short peptide with a modular
protein
• 2. When combined with the tandem zipper
formation it facilitates the closely spaced
binding of multiple copies of Fn
 How to reduce the infection?

• Combining similar residues which is present in

the fibronectin & fusing with liposome so that the
fibronectin binding protein will show high affinity
only to the liposome fused fibronectin.
• This is one of the hypothetical ways in reducing
infection
 Reference
• Yoshino C, Shiba T, Hattori M, Ogasawara N, Hayashi H,
Hiramatsu K: Whole genome sequencing of Staphylococcus
aureus. Lancet 2001, 357(9264):1225-1240.
• Baba T, Takeuchi F, Kuroda M, Yuzawa H, Aoki K, Oguchi A,
NagaiY, Iwama N, Asano K, Naimi T, Kuroda H, Cui L,
Yamamoto K, HiramatsuK: Genome and virulence
determinants of high virulence community-acquired MRSA.
Lancet 2002,359(9320):1819-1827.
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