ADEM Aquatic Toxicity Laboratory Compliance Sampling Inspection _Effluent Toxicity Test Report 1. GENERAL NPDES Permit No. _ AL 0003140 DSN: _004 County: _Shetb Facility Name Alabama Power Company ~ Gaston Steam Plant Receiving Water: Coosa River and Yellow Leaf Creek Design Flow: _-—-- Test Type: Short-term Chronic Screening Test Id. #: 080212-01 Tesi DateTime Siariag Datertime Ended Ganiol Valaty onanism YYMMOD HANA YYMMDD Hilt (accoptabierUnacceptabe) Ceriodaphnia dubia 080213 0935 (080220 1050 Acceptable Pimephales promelas 080213 1350 080220 1355 Acceptable 2. SUMMARY OF TEST RESULTS ‘Chronic Screenin, Effuent | Test Test Event | Tost Teste cone.(%) | Og. _ [SURV [ REPRO] GROW, Gone. (4) | _ Org. _| SURV | REPRO | 36lod [pass [pass | ~~ | C.. — \ Pp. | FAL | FAI P.p. 3. LABORATORY ANALYSES OF UNDILUTED SAMPLE(S) Sampo pH -lezity araness Coney TRE Sa mgtascaco, | _mglasGaco, | _pmbasion @ °c mol 080212-011 a4 108 194 597 at 23.5 nla (080214-01 80 101 187 470 at 22.7 nla 080216-01 79 405 195 s34at226 | nla 4. SAMPLE COLLECTION: Were split samples collected? No Were samples collected as specified in NPDES Permit (Location and/or Type)? Yes ‘Sample ‘Samplo(s) Colectod ‘aval To "Yad in TOSS) YYMMDO _HHIIM to YYMMOO tt 0) YvwkiDD to “YYNWDD. (080212-01 to 080212 0805 1.0 080213 080214 (080214-01 te 080214 0845 o7 080215 080216 (080216-01 ~ te 080216 0830 22 ogo2i7___ © 080219 5. CONTROLIDILUTION WATER, ‘Garboy | Preparaton Begin Use aH ‘RST, Favaness Conduety | _"YYMINDO. YYMMDD su__| mgtascaco, | males Caco, hose @ °C. cs 0207 ‘080213 Bs gy a7 280 at 23.6 ct 0208 ‘080213 a2 8 a 287 236 ca 00218 ‘soz a3 es 8 an atZa6 cs fon 080215 82 es 76 aot at229 cs e024 (080246 a 7 @ mzatZ4 cy 050236 (080218 fo oy 4 307 31229 PAGE 1 0f4PERMITTEE _APCO~ Gaslon Steam Plant NPDES # _AL.oD92340_ OSN; 004 __TESTId#: _g80212.01 6. TOXICITY TEST INFORMATION Test Organism [Organism Age. Ciganian Sauce ~[“igsRapieate | RapieaesrCons—] Ca. <24h ADEM in-house cultures 4 10 Pp. <48h Outside source 45 4 Test Orgasm | Temperature Range (O)_[ 00 Range a) pinange Gar Tphtinersty Aerage Wey Ca 233 asz_ [| 75 - 89 | 82 - 85 59 Pp. zag - 257 | 46 = ot | 75 = 83 70 7. FEEDING: Fed Daily Brine Shrimp Fed 0.45 mL. Suspension of Newly Hatches Larvae 2 Times Daily yor Fed 0.15 mL Suspension Gontaining 1800 mgll. TSS Daily Algae Fed 0.15 mL Suspension Containing 3.24 x 10" Algal CellsimL. Daily 8. REFERENCE TOXICANT TESTS TOXICANT - Sodium Chive (NaC) Test Orgarso TestDate Resale ‘95% Contonce niewat YYMMDO 1.650 (rail) engi) ca 080213 0.6881 0.6260/0.7868 Pp. It 080213 1.9190 1,3244/2.1441 9. TEST CONDITION VARIABILITY ‘A, Deviations From Standard Test Conditions: ©, dubia test temperatures were out of the suggested range on two days. C. dubia source organisms were released during a 16 hour time range from adults with approximately 15 neonates in the first seven days and =20 neonates in the first three broods from culture boards with neonate production ranges of 15-17 neonates per adult in the first seven days and 13 neonates per adult in the first six days. These deviations do not appear to have adversely affected the test results. BB. Test Solution Manipulations or Test Modifications: none Page 2 of 4PERMITTEE: _APCO Gaston Steom Plant NPDES # 10C. CHRONIC SCREENING TOXICITY TESTS RESULTS ‘TEST ORGANISM: Ceriodaphnia dubia MORTALITY CHRONIC TOXICITY INDICATED? REPRODUCTION/BIOMASS CHRONIC TOXICITY INDICATED? Test Validity: Is survival in the CONTROL > 80%? Is Average Neonates(Surviving Female in the CONTROL = 16.0? Did 60% of the CONTROL Females Produce Their Third Brood? AL ODUsI40 ON: _004 __TESTId =: _080212.01, No Sitstcat Anata Nocostary Selatan Concentration Oo) Saray Reproduction (Average # young emo Control (0%) 100 267 36 100 325 ‘SURVWAT STATIETGRL PRALVSES ang aaparton sasningrepieals 0= aes. T= ans} | COMMENTS: Contrel dala 1s from a concurrent test. REP ROOST GHTBTOMAGS ST RT SICAL ANALYSES (UBg WATE SSTSTOGIOHAT COMMENTS: 1 No Sttsca Anata Noonssary Control data 1s from a concurrent test Page 3 of 4PERMITTEE: _APCO - Gaslon Steam Plant NPDES# _AL.g0ga140_ DSN _ob4__TESTI4# _opaotz.01 10C. CHRONIC SCREENING TOXICITY TESTS RESULTS TEST ORGANISM: Pimephales promelas MORTALITY CHRONIC TOXICITY INDICATED? FAIL GROWTHIBIOMASS ‘CHRONIC TOXICITY INDICATED? FAIL Test Validity: Is survival in the CONTROL > 80%? Yes Is mean dry weight of surviving CONTROL fish 2 0.25mg? Yes ‘Sion Concenvaton Ta) Suvi ean Ty eG Control (0%) 93 _ 0.790 36 65 0.521 SOR RA STATSHEAL RAL TGES (saga ans TRE SS TRIAS | COMMENTS: Control Gala 6 ‘Shopio wks Tes (Noms) from a concurrent test. Toet Site “ccal value 0.749 (Params) a Yor (test sta ota! vate) Goto TEST iio ricetsst ie encalt) FAR TINO Gri statis =ebestt) PASS ‘GRIT TSTORSS STATISTICAL ANALYSES (eng san 5 woGDTGHTCURT COMMENTS: Control data ‘Shap wks Test (omestyy from a concurrent test oat State 008558 Creal ale 0749 (Paramstich Nema Sinaia? (ves (taste > cot value) Goto F-TEST [hn @ricststets «cea! value) Go e WILCOXCN RANK SUN TEST rest FSiataie sere ome Far a6 Yonance? Bega ifsbts ertea) Go wo MODIFIED T-TEST rarest {Statste 2258 Cctv 1842 SigntcanDifeonce? (BYES (it ataie= eel) FAIL NO estate enon) PASS Prepared by: Spend hich va 2/1] JO reviewed by. C (Nah frm Quast Sundry vad exes VA losles Page 4 of 4—__ADEM ALABAMA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT oer Ome Box H89 26100-1489 + 100 Cauceim BNO, 91102059 MONTGOMERY, ALABAMA coms “Trey” Guco Wnnabeustieal ve Boo Ruey we ion 2P1 Too conmen heen 20 ogmeee ae April 11, 2008, ae Beaaet MEMORANDUM Stas BE ee aces To: Simivo Carson Industral Municipal Mining Seetion THIROUGH: James Macindoe, Chet Water Divison FROM: Janet Branch}; AiPaclity Séctidy Field Operations Division. SUBJECT: ‘Toxicity Test Repor Please find attached Toxicity Test Reports for the following facilities for circulation fo appropriate Water Division personnel FACILITY COUNTY NPDES PERMIT Auburn ~ Northside WWTP. Lee 0050245 Auburn —- HC Morgan WWTP Lee 0050237 Opelika - Eastside WWTP Lee 0050218 Opelika — Westside WWTP Lee 0050130, Bismingham ~ Riverview WWTP Shelby 0045969 AFCO Gaston Steam Plant, Wilsonville Shelby 0003140 Results have been entered inthe ADEM Field Operations Euent Toxicity Test ‘racking System for future reference, Attachments comune BT Sr ay Beate oe a & Bag, Sea, oe Rea a rth areAAO Taival Fish Growth and Survival Tost-7 Day Biomass Sar bate TostiD 8021201 ‘Sample 1D: End Date LabID: 08021208 © ‘Sample Type: ‘Sample Date: Protocol: EPAFWO2-EPAVS21/R-02-01 Test Species: _PP-Pimephales promelas Comments: Gone 4 z 3 4, B-Control OATO7 OBG00 —08H20 O.57S7 36 0.3203 0.7721 05327 0.4813 “Transform: Untranstormed Tailed Gonc-% _{ Mean \v-Mean Mean Min Max CV% _N t-Stat_Gritical_MSD B-Control] 0.7898 | 7.0000 0.7895 0.5757 0.8900 18.615 4 "36\ 0.5214 ) 06603 05214 0.3209 0.7721 35.043 4 2256 1.943 0.2810 / ‘asiay Tests Siatlstic Griial Skew Kurt Shapito-Wik’s Toat mdicaes normal Getibufon (p> ODT) 0.95506 0.789 3.01885 04579 Test indicates equal variances (p= 0.70) 1.61703 a7.4672 Fypothesis Test (tai, 0.03), MSDu —MSBp— NSB —MSE—F-Prob af Homoscedastic t Test Incleatyr Scant erences 0.23102 0.20258 0.14389 0.02827 0.06489 1,6 Treatments vs 8-Contol Page 1 ToxCale v5.0.23 Reviewed by:Tarval Fish Growth and Survival Test? ay Survival ‘Slant Date Festip: 8021207 “Sample 1: End Date: Labi: 08021208 0 ‘Sample Type: ‘Sample Date: Protocok EPAFWO2-EPAV821/R-02-01 Test Species: _PP-Pimephales promelas Comments: Gone-% 1 2 3 4 B-Contiol T0000 OBSTT 0.9333 0.9285 38 0.3333 0.8867 0.8667 05333 i "Transform: Arcsin Square Root ‘Tailed Gone-% {| Mean \W-Mean Mean Min Max CV% _N_t-Stat_Critical_MSD B-Convoll 0.9208] 7.0000 1.3086 1.1832 14413 8.0054 "3.8 08500 / 0.6991 0.9571 06155 1.1970 30.220 4 2284 1.943 0.2991 N Auxiliary Tests: Statistic Gritical ‘Skow Kor ‘Shapro-Wiik’s Test indicates normal dstibuton (p> 0.01) 0.98044 0.748 "0.3375 0 5025 F-Test indicates equal variances (p = 0-13) 7.51018 47.4672 Hypothesis Test (1-ail, 0.08) MSDuMSDp__MSB___WSE__F-Prob af Homoscodastict Tost indicaips significant dWferences 021617 023174 O247I7 0.04738 0.08249 1,5 “Troatments vs 8-Control A ome Toxaev60.23 rovowet JAOC [Test UF-Larval Fish Growth and Survval Test "Fast 1D: O80RTZ07 Species: PP.Pimephales promelas Protocol: EPAFWO2-EPAI821/R-02-013, | Sample 1D: ‘Sample Type: Start Dato, End Date: Lab ID, 08021208 © Toul | Tare | Wot Pos| 10 |Rep| Group _| Day 0| Day +] Day 2| Day 3| Day 4] Day 5| Day 6|Day 7] watig) | Wawa» | Count ait B-Convil, 15] i5| (1.01878 1.00603] %5| 2[2 B-Contol] “7| 6 j1.02828] | 1.02205[ 7] 3] 3 B-Contoll 15] a] [1.03559] 1.02236] 75| 4,4 B-Control| S14] 13] /0.98843]1 0.96037] ~ 14 So 3.600] 15] 5| 0.98773}. 0.98279] 45| e[2 3.600] 15] 3] 1.01628] 1.00547| 14 713 3.600] 15] 13], 1.00678} | 0.99879] 15| al 4 3.600] 15] ali 1.00934] 1.00257] 45] Comments: Le Poge 1 ToxCale 5.0 Reviewed by:[Fost LF Larval Fish Growah and Sunival Tost Tost 0; BORT2OT ecies: PP-Pimephales promelas Protocol: EPAFWO2-EPAI821UR-02.013 Isami Sample Type End Date: Leb ID: 08021206 6 Total] Taro] Wot Pos| 10 | Re [Day 0| Day 1] bay 2} Day 3] Day 4] Day 5] Day 6|Day 7] Wo'g) | Wat) | Count +[4 15] 48| torszal1.00863[ 2 [2 | s-Conteoip> | 1.02828) 1.02205 S13 [—8Coneil 15 74] 103650] 1.02236[ 15 [4 [8 Contra 14 43] 0.96843] 0.56037| 1] s(t 3.600] 13 = {0.987731 0.96279] 15] 62 3.600] 15] aS 73[3.001626] 1.00567| 14] 73 3.600] 15] = = 3{* 1.00678) 0.90879] ta] aps 3.600] 15] eT = el, .c0934) 1.00257| 15] Comments: - 5 Page 1 ToxCale 5.0 Reviewed by:rest LF-Uatval fish Growth and Sunvval Test Test 1D, 502127 species: PP-Pimdprales promelas Protocol: EPAFWO2-EPAI62 VR-02.013- Same IO: Samplo Type: Start Date End Date Lab ID: 08021206 6 Total | Tare ] Wot ros] 10 [eo] croup _|oay say 1|oay2}02y 3|o8y 4 o2y 6) Dey | 0ay74 wate | wate) | count Ti | econo — 15] i5{_107878) 100662] 15} 22] e-Controll — 7| al 02828] 1.02208) 7| 3 [3 | 8-Controll 15] 74] 7.03550[ 1.02236] 75 a [4 [—8Contrll 14] 3] 0.98843] 0.96037] 14 St 3.600] 15 3] 0.98773] 0.96270 15) e[2 3.600] 15) 7a] 7.001628] 1.00587} TY TPs 3.600] 15) = s Ta] 1.00676] 0.99879] 15) ape .600 15 2 | 1.00834]_1.00267] 15] Comments: \ “ (\eAding BEANE fy fo OL, 444 Pare {aWal Fah Growl ad Suivival Test? Day Biomass att fete Sian Date 1D 08021 20 ‘Sampo 1D End Date Lab Ir —08021208 © Sample Type: ‘Sample Date: Protocol: EPAFW02-EPA/G21iR-02-01 Test Species: __PP-Pimephales prometas Comments ‘Cone-% 1 z 3 a B-Control O80? 8800 08620 05757 36 0.3400 0.7836 0.5433 0.4820 ‘Transform: Untransformed Tailed Gone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD B-Gontsol 0.7898 1.0000 0.7896 0.5757 0.6000 18.615 4 "36 05322 06740 0.5322 0.3400 0.789 35.181 4 2,162 1.943 0.2913 Auxiliary Tests Statistic Critical Skow Kart ‘Shapio-Wik's Test indicates normal Gstabubon (p> O07) 0.85564 0.748 D022 0.4595 F-Test indicates equal variancos (p = 0.70) 1.62203, 474672 ‘Hypothesis Test (tail, 0.05) MSDuMSDp__MSB_—"MSE_F-Prob__af Flomoscedastict Test indicates significant aferences 0.23128 0.20207 0.13248 0.02833 0.07383 1, 6 Treatments vs B-Control Page 1 ToxGale v5.0.23 Reviewed by:Facilty Name: DAY # and Date (yymmaa) “Test lait: 080212.06 ING a 7 z 3 4 3 3 7 TONE REP] ovo213 | oeozia | osozs | osozi6 | osozi7_| oeozie | osozie | 080220 3 7S Ee a 1S is 15 1 0 10 15 1 T T a G é At Ly ver Ty imi wy 4h 12 na L ts Be 43 ~ J £ 2 s 1S) 7 100 ir a ir Ts 76 15 im is 7 fe 1S = 18 io Tse t ee SISA |S ] TECH [raz | 1095 me eo rs OS [5 is90 TIMERANGE | \240-ta roy |'® 1030 Mas "AM_ FEEDING TECHITIME PM FEEDING B Aw TECHITIME VSED] 1530 1Goo Fed 0.16 ml Suspension of newy hatched brine shrimp larvas Recoid number of erganisms alive at each observation and note any anormal Times dally, lies or missing organisms. ‘Comments: ap K8 ed Ve 1 AS RANDOM LETTER Pimephales promelas CHRONIC SCREENING GROWTH DATA Cc re Bevery amet mo) e Ty conc. | REP.# | WGT.OFBOAT(g) | BOAT+LARVAE(g)_| FINAL #LARVAE 9 LCCCER else 16 0 10 Logie TOs SIS é 1 RESTS pe ass A 72 CECT 16d 3 ir O47 TF: we OG I 1S 100 * GSCcL ont 15 45, Lee f OjO36 1 16 o2ses eC37Et BaF Ternger WA Dying Start CeeaTs 56 [OVOP [334,, oueriwareaitenp | rue see ae 1d Drying End e8oL1F leds leforz2; CF3- DatemTime/Tech Temp ye lowe [oes fev" Weighing Chang /edo | Opener, OFer Date/Time/Tech HAS. yg Brying Oven Used 74-03557 “Temperature (°C) FOD |-FORM 254 (REV 05/17/07)Facility Name: Alabama Power Company - Gaston Stream Plant testa C YOAIQ-ol Pimephales promelas CHRONIC SCREENING SURVIVAL DATA & FEEDING. DAY # and Date (yyrmmaay a 7 z 3 4 3 3 7 oso2a | oso214 | ceozts | oscz1e | asozt7 | osozs | osozia | o¢oz20 Concurrent 1s Control DS | 2 5 = 1S Ss 5 5 36 30, 45 1S is iW if 12 13 13 EH Ns Sig 13 [1s 7 re 32 {OS ses ef ——_t Teon TAIS-| DS [HS Cod iozo~_ | VO> [Saga [TsoST TIME RANGE preg | ne LOA [yg 1035) [pg ors | er 100 AM. FEEDING ee % ge fae We TECHITIME emo | desserts vray | 0749 | ets PM FEEDING | | a ree, ee OC é TECHITIME a. |rss0 | vaae | rds” 1938 | is ice oP 13>. Fed: 0.16mi suspensionv6r newly hatched brine shrimp larvae 2 _ times daly. Recoid number of organisms alive at each observation and note any anomalies or missing organisms. Comments: Pimephales promelas CHRONIC SCREENING GROWTH DATA Ay i WGT.OF BOAT (g)_| BOAT + LARVAE (g) 7 FINAL # LARVAE conc. | REP. # Concurrent! Control 16 Ol6Rk. Bank Fa Drying Start DaterTime/TechTemp Ta rage uD 138 ¢ los 200 soy" OFe2g2, DFIC nus tove Of o2a/ Ofer Lor aae Croale 1158 mG gyre eBOnT 1026 rH love Weighing ebcat pave Date/timefTech | 5x. ee. Drying Oven Used: 74.03557 Temperature CC) Gore: Ck Concise, OT ake line, FOD I FORM 264 (REV 05/17/07) co Drying End DoteTime/Tech’TempFacility Name: Alabama Power Company - Gaston Stream testi OYOR/2Q--/ Ceriodaphnia dubia CHRONIC SCREENING SURVIVALIREPRODUCTION DATA DAVE and Dale Gymnas) 7 2 3 4 5 6 7 REP? Josoz14 _|osozts _Joso216_|o80217_|oso218_josoz19_|ogo220 2 S| 2 8 é RENEWAL [REO | 495 [ogee | Ssons| eo [OE Tecnitme [07g | own) BF fees | ES) sw ty FEEDING Ju Ens” y are oe tecnime _fosso_| guy t Ofe sw \ Start Time/Tect: /1/0 $8. Dayo Feeding Timertecn:;__ OF O G6 Comments TEMPLATE NUMBER (or random letter) C Legend: y= organism alive M= Male X = organism dead -# = dead neonates L= Lost or Missing organism CONG:: All concentrations are % effluent. Split broods indicated by circling both neonate counts together. Fed: 0.15 __mi suspension of YCT containing 1800 mg/L TSS and 0.15, mL suspension of algae containing BF 3.28 x 107 ce FOD I-FORM 255 (REV 05/14/07) 80802 12-08 CoC EVEL snl DAY # and Date (yymmaa) 7 2 a a 5 5 7 CONC _[REP-FJoso2'z |ogo215 |oso21s _fosozi7_|osoz18_oso219_|80220 moe oes 7 Ce 22 iz TT 23. 24 2 25 27. 28 29, 30 El 32 3a 34 Ed 36 37 38 39, 70, RENEWAL 3 (PRS [ors [bss TECHITIME 15.0 ONL], FEEDING | 3 = es TECHITIME 0830, ost? | oss OF10 start Timertech: | | Sk Day 0 Feeding TimefTeeh: ‘Comments: NUMBER (or random letter} Cc Legend: y= organism alive ive neonates X = organism dead jead neonates CONC.: All concentrations are % effluent, Split broods indicated by circling both neonate counts together. Fed: 0.15 __ml suspension of YCT containing 1800 mg/l. TSS and 0.15 mL suspension of algae containing 3.24 x10" celis/mL. daily FOD |-FORM 255 (REV 05/14/07) Male Lost oF Missing organism uCm 63h t ‘Coriodaphnia Survival and Reproduction Test Reproduction ‘Start Date: Test iD: 8021201 ‘Sample ID: End Date Lab 1D: Sample Type: ‘Sample Dete: Protocol: EPAFWO?-EPAI821/R-02-01 Test Species: _CD-Ceriodaphnia dubia Comments: ‘Cone-% 7 z z a 5 e T z 3 ci E-Contral 0.000 23.000 30.00 31.000 31 000 78.000 25 000 30.000 33.000 36.000 3.8 29.000 35.000 38.000 37.000 33.000 34.000 25,000 34.000 37.000 23.000 Rank 7-Failed Conc-% _f Mean W.Mean Min Max CV N Sum Critical ‘B-Contral] 26.700 | 7.0000 26.700 0.000 36.000 87.787 10 36\ 32500 }1.2172 32.500 23.000 38.000 15908 10 12850 82.00, Runaiary Tests. Statistic Critical ‘Skew Kurt ‘Shapiro- Wilks Test indicatos non-normal distibution (p= 001) 0.77432 0.868 “2.3007 6.82874 F-Test indicates equal variances (p = 0.06) 3.80915 6.54109 Hypothesis Tost (1a, 0.05) Wilcoxon Two-Sample Test indicates ho Significant aMferences Treatments vs B-Conttol —_ Page 1 ToxCale v5.0.28 Reviewed by:Test lait: 080212-08 Facility Name: DAY Fang 0 7 2 3 4 5 6 Cone. ogo21s_| osozia | oso2is | oso216 | oso217 | ogo218 | oso219 Temp. jo. M3 [A To eT OY, ° bo. | st | 7S ani wo | 4-4 pi Sa ive) 8.9 Teme | a3 a | 4100 ‘ aa pH Tem D.O. pH Sy oor = TE SE = Pimephales promelas CHRONIC SCREENING FINAL WATER CHEMISTRY DAY E Concentration 4 z z Temp, Que er Te ° D.O. i A GO : pH 7 4 wen, Jad. F125. aos f 2 5, 100 ot PI pa 7, : 4. : 7 Temp DO. pH Ceriodaphnia dubia CHRONIC SCREENING FINAL WATER CHEMISTRY DAY # Concentration 7 z 3 4] 7 3b Temp. | 2.5 | ao oy. DS. 254 “ 0 DO. aS Go % ca : gM pH sa 3 ey a 2 S35 od) lass Ss S2 #6 100 DO. a 4 pH & Temp. B.0. DH. Stat Time es Jouse [9S los End Time 13S 055 [ese [cc v 1635 110 > beat 2 Grd Tals 2 reamicars PS fue eps [eh a, |RSS Ma PRT p 4 dF | TW | oe Temperatures (°C): D.0. Meter, D-3, 0-8, D-2, D-7 Al convonttaton rcosded'as % ofuent. + From Chemical Determination NR: Not recorded, technician ecror C;, FOD FORM 200 (REV 05/1407) mFacility Name: Alabama Power Company - Gaston Stream Plant Test tO f CHRONIC SCREENING INITIAL WATER CHEMISTRY, DAY # and Date (ymmdd a i 2 3 4 3 @ 080213 990216 | 080217 | osozia | ogozt9 Pimephales promelas CHRONIC SCREENING FINAL WATER CHEMISTRY DAY # z z 4 5 a Sy 15 Ceriodaphnia dubia CHRONIC SCREENING FINAL WATER CHEMISTRY DAVE ‘Concentration 7 z 3 4 5 3 7 Temp. —S Concurrent Contd [D0 aad [eso [ass So [att U9 5 271 ee | ee eat a ST DAVE o TT z 4 5 7 Stat Time | 100 Cus [130 Kal oA End Time (020 | OVS | WIR | [00s Tit] F400 Ta [PGT SX |e hyo Tein Slav a BEES ‘PH (Su) meter D.0. (mah) meter: 7400873 Temperatures (°C): 0.0, Mete All concentrations recorded as % effluent. NNR: Not recorded, technician error FOD FORM 260 (REV 05/14/07) . D7 * From Chemical Determinations LiOwe OeCaizZ a) Cboata-Cl, OLUSTL-OT ORGANISM SOURCE INFORMATION Test Id. #: Date Tests Started OS O13 TOXICITY TEST INFORMATION ‘Test Organism | Organism Age | Organism Source | _Fed Wile Holding? | Org/Test Vessel | RepicatesiConc, [e dubia a t P_promelas eS ORGANISM SOURCE - u Ceriodaphnia dubia a Pimephales promelas Brood Board Id/Dale: Domne Oh Tech ores YO Retease datetime range Se aes i Technician Brood Board Cup # Test Board ROW # 0° CUP # dG D t = Aquarium I Q a COTY 5 RH] ater socritsrbage’_ a Va G 4 5 + INCUBATOR TEST CONDITIONS Tneubator 7 incubator 2 Temperature] Light intensity [Temperature] Light intensit Date/TectTime | Contents [Og | Mid | Top | Mid [ Bot | Contents| Dig | Mid | Top | Mid | Bat “ ( tas Incubator 1: ADEM ID# ‘Thermometer Id Temp Recorder Id Incubator 2. ADEM ID# Thermometer Ia Temp Recorder Id LEGEND. _ Temperature - All temperatures are recorded in °C. Dig - Digital (digital temperature reading obtained from each incubator control box) Mid - Misdle (temperature measured with a glass or digital thermometer on the middle shelf of the incubator) Light intensity All light intensity recorded in foot-candies using light meter # Top - light intensity measured in center of top shelf of incubator Mid - light intensity measured in center of middle shelf of incubator Bot - light intensity measured in center of bottom shelf of incubator Dates - yymmdd Time - 24-hour time notation FOD FORM 252 (REV 05/17/07)Testid# ORGIIA-C5, OROAIQ-63 Date Tests Started OC @C.Q\3. OBG BAA OB, CV OAWI-O4 ORGANISM SOURCE INFORMATION ‘TOXICITY TEST INFORMATION Test Organism | Organism Age | Organism Source | Fed Whie Holding? | Org/Test Vessel | RepleatesiCone [E_dubia adh Tes Auer feo, 7 2 [P._promeias £36 AR Ae: e 1 4 ORGANISM SOURCE Ceriocaphnia quia 55) A-1 ORAS) Pimephalos promeles Brood Board Id/Date: DOSOIY ASE” Tech: QA Release dateltime range: p aly Weary alr o WS . NANA Kay C72T Technician: QS Yrs Beood Board,Cup # \-\ a\1a Pest Bard ROW a or CUP # ~ 3b Sel 38 wi Aquarium 6: LOFLQ{3 ~ 2 [dD al a Hatch datetime range: a= (Daa MSF HH Lhirleg P730- 1100 CST Cd wig Aetenae dentin, tlle A (GK chine Tange, Ne CA Scone Big Bao ends Woes arose ony Gathegee ane toe tint Bde ate Bin SESE ES ea Sem ange aaNOUBATORTEST SONDIMONS Tyrie ter tS a Ty eae a Thewator 7 Theubator? [Femperaturel —Lightintensity FTemperaturel —Light intensity DatofTechitine | Contents [Dig | mid [Too | Mid | Bot| Contents [ig | Mia | Top | wis | Bot lecoav S20) eR a9- as. 4 [7 ean) Op [Fis [as4]45 [59 1 64 ceeatd fis oust ea) CRBs facia 5 Tied fom )° Sb leiorfaeol4a Tee [EO ssoars [ye tse], las e134 tsi Rozvlvtis 4a 1S ¢ |e om 53 re 1 : = Kosar 6 axl oralya e ee Bika he fez. J? BEE Sates e190? tvelous Ya ISM led sere] Sse Fo [aa ws[ave] 41 L312, tea 17 pad Skee ealesstes oe Firlg-el¥a | safe a 1200 Key cms | SS Doe b foo] g.5 thy [30 fee [Sy les ~ ES = Incubator 1: avemip# (10745 Thermometer td Temp Recorder Id _ 2 Incubator 2: ADEM ID# “7=OLS5q Thermometer Id / Temp Recorder id “fe LEGEND ‘Temperature - All temperatures are recorded in °C Dig - Digita (digital temperature reading obtained from each incubator control box) Mid - Middle (temperature measured with a glass or digital thermometer on the middle shelf ofthe incubator) Light Intensity All ight intensity recorded in foot-candles using ight meter# Gove SUN I® SMOU 3s 4¢ Top - ight intensity measured in center of top shelf of incubator * Mid - light intensity measured in center of middle shelf of incubator ot - light intensity measured in center of bottom shelf of incubator Dates - yymmad Time - 24-hour time notation FOD 1 FORM 282 (REV 05/17/07)c zo.11 28.28 2577 24.94 451 Fed ————________Ineuhator #2 C.d, tests 080213 and Pp Temperature ~ RTDTempi01 - M55437 R2 | T | | 1:22 Pe 42550 +098 Pm sas pm ont pu 2440 pm ‘7008 fe 42008 16,2008 Feb 10,2008 Feb 20,2008 feb 22,2008 Ea os or or & aDevice - RTDTempt01 ~ — Incubator #1 P.p. 080213 start, Serial Number = 55436 Temperature: - RI 28 - =; 1 I | i en \ - | | | 4 ! i — fa { L 25 { a | os j t \ , — a at bene » 12:00:00 am 12:00 Pm 2.2400 PM 9:26.00 aM 448.00 aM 1200:00 am Fob 12,2008 Feb 13, 2008 Feb 19,2008 Feb 17,2008 Feb 10,2008 Fp 24,2008 cst ost csr cst est ostFacility Name: Alabama Power Company - Gaston Stream Plant Test Id#: OF OD ID l Sample Preparation Information Concentration (%) | Quantity Required (mL)|_Effluent Volume (mL) | Dilution Water Volume (mL: ° 1200 0 4200 1500 54 1446, —<— a <= Sample Id. # 080213 soon OF 00 Yb 80214 SOQ a) OPOMS-o ofeais-a} myoois -ol as | 080218 FOD FORM 250 (REV 04/13/07)CHEMICAL DETERMINATIONS LOGSHEET Tempo | oars] Teow TAURALINTY (wat a5 C200) HARDNESS [nal s3 C000) conoucTIviy TEP sawp ioe | cone sea | me [onesugpot concervaton [nt tad muitgrarconcentoton | OMpgrem ee) Te ogo212-o} [3.6 losozis | es4o] F-3] 2c x05 - 6% Gi x00 - 9 Fa? a _ logoria-0 1 |100 _fosors_| OS |! |ic.s x05 - fer | ria x 100. 194 S77 ogoald-o\|ao fosoris fivs |2.a] exe - FI [Wb . 0 . I asi Jog c214-o1 [100 __fosozis 6.0] 4G 105. 101 (8.7% 10 - (eT ATC g 9 anre-o\ foe losorre 6? 16.6 .ws - CFT [87.10 . $7 So0F oSo216-01|r00__fosozis 14 - 105 | 1S. wo - 195 | 554 ~ x 10.0 ™ so ™ 1105 = 400 - e x 105 1100 - e ‘Aikalinty Titrant Batch # Hardness Titrant Batch # pH Meter ID # Conductance Meter ID # TRC Colorimeter ID# (Zor2Ls0 Aa) 9% WYO+1 GOS 080130 080130, "74-15430 7atat71 THA 195 ‘eig uieang uo|seD - AuedwWog laMog eweqely OWEN Aloe V-Et COZo wiserADEM Aquatic Toxicity Laboratory SAMPLE COLLECTION / CHAIN OF CUSTODY RECORD. racity: Alabama Power Company - Gaston Stream Plant |contac: Sygeas Cozuen Location: Wilsonville Shelby County none: em ZEEE" INPOES PemitNor Jat 0003140 losw: 004 IRBRISH ACHE ype of Sapte: Grab Discharge Fiow Sampiei0 # 2nd Read Sample fom: Date brine sot Reading Samat: Dae rine Same cosecod by: Hine Lett tt tite Date: 080212 re: 90-5 FLow I4-6 MED commen: Sample Containers Number 2 si 4/2 gallon Lype Plastic Test Type: Chronic Screening Tota Residual Codne IWC %: 3.6 Receiving Water: Coosa River and Yellow Leaf Cree lino Design Flow: -— Mater # 74-14196 Relinqushed Dote Tine Receives efatet 1235 \niox Aecian Betabott, Receiving Temperature °C FOD FORM 266 (REV 05/17/07) laboratory INE OF D2sz <0ADEM Aquatic Toxicity Laboratory SAMPLE COLLECTION / CHAIN OF CUSTODY RECORD. recy: Alabama Power Company - Gaston Stream Plant conace Bycen Cepinn Location Wilsonville Shelby County. Prone eons YAS $043 [NPDES PemitNo: _|a._ 0003140 losw: 004 (Sue 1G ete lye of Samp: IGrab Discharge Flow [sampler 104 lend Reading Sample rom ate Line st Reasing Same bate: rine sence ctestes vy WMessne Aci fleee ie Date: 080214 frime: Cee [row Icomment: Sample Containers Ihumber 2 sce 4/2 galion Type. Plastic Test Type: Chronic Screening Total Resivai Coxine IWC %: 3.6 Receiving Water: Coosa River and Yellow Leaf Cree. laity Design Flow: — hdeter 10 # 7414196 Resoquished Date Time Received Afifes 1218 \wra Marcas Lie Tat ate, [Receiving Temperate e7 °C) FOD |-FORM 266 (REV 05/17/07) l.sborato oo: CS C2) e/ADEM Aquatic Toxicity Laboratory SAMPLE COLLECTION / CHAIN OF CUSTODY RECORD LEE CLR wee Facility: Alabama Power Company - Gaston Stream Plant [contac Byree Coen a |Location: Wilsonville Shelby County Prone: 2 OS ATF FOF F INPoES Pemitto: Jat 0003140 losn: 004 pe isme Fete [Type of Sample: [Grab Discharge Flow sampler # Jno Resding samp tom [bat rine ist Reading sampie [bat rine: sent ote Massa Aertabte user [Date: 080216 lime: OF 30 [FLOW looms: Sample Containers [Number 3 size 1/2 gallon brie Plastic Test Type: Chronic Screening ‘Total Residual Chlorine we %: 3.6 Receiving Water: Coosa River and Yellow Leaf Cree fimory Design Flow: —- [Motor |0 # 7174-14196, retnavanec oaeitine ——_[Raceiee ile 1-20 \nise Mar Macbibin te [Receiving Temperature: 22 °C] laboratory ID Nor OF 2 7h L FOD L-FORM 266 (REV 05/17/07)