Abstract:

Modern biotechnology owes much to its roots derived from plant tissue culture and
micropropagation. Indeed, the landmark publication by Gottlieb Haberlandt (1902), who
is arguably referred to as the "Father of Tissue Culture", is often cited as the origin and
emergence of plant tissue culture and its subsequent applications. Success of
biotechnological approaches is dependent on regeneration of intact plants following
genetic modification, generally by micropropagation. Contributions of Knudson,
Thimann and Went, White, Gautheret, Skoog and others are discussed in a historical
perspective. Examples of currently employed uses of plant tissue culture, especially
practical applications of micropropagation are presented, which enables science to
transition into an evaluation of what lies ahead. Potential uses of plant tissue culture and
biotechnology to further our understanding of plant physiology, how plants function and
resolution of legal issues are presented. Agrobacterium-mediated genetic transformation
along with other modes of transformation such as microprojectile bombardment have
been accomplished. Incorporation of disease and stress resistance and other
horticulturally important traits is a logical outcome to be anticipated in the future. Use of
molecular technologies for identification of genotypes, clones and their ancestors will
enable researchers and producers to verify identity and parentage of propagules, whether
produced by conventional or modern propagation methods. No doubt yet to be imagined
applications of plant biotechnology will emerge as the XXIst Century continues to unfold.