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A portable, inexpensive and microcontrolled
spectrophotometer based on white LED as light
My Applications
source and CD media as diffraction grid
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Germano Verasa, b, Edvan Cirino Silvaa, ,
, Wellington Silva Lyraa, Sófacles Figueredo
Carreiro Soaresa, Thiago Brito Guerreiroa and Sérgio Ricardo Bezerra Santosc Table Download
a
Universidade Federal da Paraíba, Departamento de Química, 58.051-970, João Pessoa, PB, Brazil
b
Universidade Estadual da Paraíba, Departamento de Química, 58.109-753, Campina Grande, PB,
Brazil
c
Centro Federal de Educação Tecnológica da Paraíba, 58.015-430, João Pessoa, PB, Brazil
Received 17 April 2008; revised 18 August 2008; accepted 19 August 2008. Available online 27
August 2008.
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Abstract
A portable, microcontrolled and low-cost spectrophotometer (MLCS) is proposed. The instrument
combines the use of a compact disc (CD) media as diffraction grid and white light-emitting diode Cited by (1)
(LED) as radiation source. Moreover, it employs a phototransistor with spectral sensitivity in visible
Analytical chemistry in Brazil: Healthy and growing
region as phototransductor, as well as a programmable interrupt controller (PIC) microcontroller as Journal of the Brazilian Chemical Society
control unit. The proposed instrument was successfully applied to determination of food colorants View details of all 1 citing articles in Scopus
2+
(tartrazine, sunset yellow, brilliant blue and allura red) in five synthetics samples and Fe in six Provided by Scopus
samples of restorative oral solutions. For comparison purpose, two commercial spectrophotometers
(HP and Micronal) were employed. The application of the t-paired test at the 95% confidence level
revealed that there are not significant differences between the concentration values estimated by the Related reference work articles e.g. encyclopedias
three instruments. Furthermore, a good precision in the analyte concentrations was obtained by
LIGHT EMITTING DIODES
using MLCS. The overall relative standard deviation (R.S.D.) of each analyte was smaller than 1.0%.
Encyclopedia of Modern Optics
Therefore, the proposed instrument offers an economically viable alternative for spectrophotometric
Semiconductor Telecommunication Lasers
chemical analysis in small routine, research and/or teaching laboratories, because its components Encyclopedia of Materials: Science and Technology
are inexpensive and of easy acquisition.
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Keywords: White LED; CD media; Programmable interrupt controller (PIC) microcontroller; Visible
spectrophotometer; Food colorants; Iron (II)
Article Outline
1. Introduction View Record in Scopus

2. Experimental
2.1. Reagents, solutions and samples
2.2. Description of the proposed instrument

3. Results and discussion

1 of 2 2/24/2005 5:49 PM
ScienceDirect - Talanta : A portable, inexpensive and microcontrolle... http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T...

3.1. Calibration of the MLCS optical–mechanical system

3.2. Determination of food colorants in synthetic samples
3.3. Determination of Fe2+ in restoratives oral solutions

4. Conclusions

Acknowledgements

References

Corresponding author.

Talanta
Volume 77, Issue 3, 15 January 2009, Pages 1155-1159

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