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Abstract This paper aims to examine aflatoxin removal ability of four species of lactobacilli. The lactobacilli were incubated with aflatoxin B1 (AFB1) dissolved in PBS for 30 minutes. After incubation the toxin residual in the supernatant was determined. Reduction of the toxin quantity in the liquid medium was performed by all species. Binding of aflatoxin with the species varied from 0.46 to 34.60%. The significance of aflatoxin removal extents were discussed from practical aspects. These results further support the examination that a number of probiotic bacteria are able to bind aflatoxin B1, examinations that opened a new window for many developing countries which are not afford expensive technologies to eliminate aflatoxins in feed supplies. Key words: aflatoxin B1, Lactobacilli, toxin-binding