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  • A3 - Antibody Base ELISA

    Enviado por

    Nica Rosales Sibug
    10 visualizações10 páginas
    Antibody base test(ELISA) Subsec A3 INTRODUCTION: IMMUNOLOGY Antibodies recognize intruder moleculesspecific! Attach to foreign bodies - more recognizable Used in research and disease diag and treatment. ELISA detects Ag / Ab labeled with an enzyme marker proportional to the concentration of the Ag mx spectrophotometrically / naked eye highly sensitive and specific.

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    a3 - Antibody Base ELISA

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    Antibody base test(ELISA) Subsec A3 INTRODUCTION: IMMUNOLOGY Antibodies recognize intruder moleculesspecific! Attach to foreign bodies - more recognizable Used in research and disease diag and treatment. ELISA detects Ag / Ab labeled with an enzyme marker proportional to the concentration of the Ag mx spectrophotometrically / naked eye highly sensitive and specific.

    Direitos autorais:

    Attribution Non-Commercial (BY-NC)
    Baixe no formato PPT, PDF, TXT ou leia online no Scribd
    Sinalizar o conteúdo como inadequado
    10 visualizações10 páginas

    A3 - Antibody Base ELISA

    Enviado por

    Nica Rosales Sibug
    Antibody base test(ELISA) Subsec A3 INTRODUCTION: IMMUNOLOGY Antibodies recognize intruder moleculesspecific! Attach to foreign bodies - more recognizable Used in research and disease diag and treatment. ELISA detects Ag / Ab labeled with an enzyme marker proportional to the concentration of the Ag mx spectrophotometrically / naked eye highly sensitive and specific.

    Direitos autorais:

    Attribution Non-Commercial (BY-NC)
    Baixe no formato PPT, PDF, TXT ou leia online no Scribd
    Sinalizar o conteúdo como inadequado
    de 10

    Antibody base test(ELISA)

    Subsec A3

    INTRODUCTION: IMMUNOLOGY

    Study of the immune system 3 fundamental functions

    To recognize intruders To respond appropriately To respond next time

    INTRODUCTION: IMMUNOLOGY

    Antibodies

    Recognize intruder molecules

    specific! Attach to foreign bodies more recognizable Used in research and disease diag and treatment

    INTRODUCTION: ELISA

    Enzyme Linked Immunosorbent Assay

    Immunoassay --- detects Ag/Ab labeled with an enzyme marker proportional to the concentration of the Ag mx spectrophotometrically/ naked eye highly sensitive and specific

    PROCEDURE: Competitive ELISA


    1. Pipet Sx, CN, CP to corresponding wells Wash 2. Pipet Primary Ab

    Wash
    3. Pipet Secondary Ab Wash 4. Pipet enzyme substrate (SUB)

    PROCEDURE: ELISA

    POSITIVE

    NEGATIVE

    RESULTS:

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