GTP Lysis Buffer Recipe (500 mL): 5.9575g HEPES (add to 100 mL dH2O and pH to 7.5 first) 4.

383g NaCl 1.25g Sodium Deoxycholate 5 mL 100% NP-40 (Schooley Lab) 50 mL Glycerol 50 mL 100 mM MgCl2 (Kristys shelf in deli) 2.5 mL EDTA Bring to 500 mL with dH2O Tris Buffered Saline, 10x (1L): 80g NaCl 2g KCl 30g Tris-Base Bring to 800mL with dH2O pH to 7.4 with HCl Bring volume to 1L