Chemicals and Biochemicals Required

Enzymes 1 Peroxidase 2 Gluco oxidase 3 Cutinase 4 Protease Enzymes activity assays (Reagents to be used) 1 Amylase Soluble starch, Citrate-phosphate buffer (pH6.5), 3, 5, dinitrosalicyclic acid, NaOH, Sodium potassium tartarate 2 Pectinase Citric acid/Phosphate buffer (pH 5),Anhydrous sodium carbonate, Glycine, CuSO4.5H2O, Neocuprine-HCl, D-galacturonic aicd, Polygalacturonic acid 3 Lipase Phosphate buffer, pNPP solution, Na2CO3 4 Cellulase Sodium acetate buffer (pH5.5), Carboxy methyl cellulose, 3, 5, dinitrosalicyclic acid, NaOH, Sodium potassium tartarate 5 Catalase Potassium phosphate (pH 7), Hydrogen peroxide 6 Peroxidase Potassium phosphate buffer (pH 7), Hydrogen peroxide, 4-Aminoantipyrine, Phenol 7 Gluco oxidase Potassium phosphate buffer (pH 6), o-dianisidine, per oxidase, glucose 8 Cutinase 9 Protease

Estimation of fabric contents, substrates and hydrolyzed products 1 Cellulose Acetic acid, Nitric acid, Anthrone, Sulphuric acid 2 Viscose 3 Polyester 4 Glucose Estimation of reducing sugars by DNS Method Dinitrosalicyclic acid, crystalline phenol, Sodium sulphite, NaOH, Potassium sodium tartarate, 80% Ethanol Phenol Sulphuric acid method for Total Carbohydrate Phenol 5%, Sulphuric acid 96%, Sodium carbonate 5 Starch Anthrone, 95% Sulphuric acid, 80% ethanol, 52% perchloric acid, glucose 6 Poly vinyl alcohol 7 Pectins Gravimetric Method Acetic acid, Calcium chloride, Silver nitrate, NaOH, Phenophthalein indicator Colorimetric Method Ethyl Alcohol, Zinc dust, Sulphuric acid, Potassium hydroxide, NaOH, Sulpuric acid, Recrystallized carbazole, Purified alcohol 8 Lipids (oils, fats, Petroleum ether wax) 9 10 Hydrogen peroxide Proteins Potassium per manganate

11

Molischs test

1-napthol, alcohol, Conc. Sulphuric acid

List of Chemicals
Sr. No. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 Chemicals Soluble starch Citrate-phosphate buffer (pH6.5) 3, 5, dinitrosalicyclic acid Sodium Hydroxide Sodium potassium tartarate Citric acid/Phosphate buffer (pH 5) Anhydrous sodium carbonate Glycine CuSO4.5H2O Neocuprine-HCl D-galacturonic aicd Polygalacturonic acid pNPP solution Recrystallized carbazole Sodium acetate buffer (pH5.5) Carboxy methyl cellulose Purified alcohol Potassium phosphate (pH 7) Hydrogen peroxide 4-Aminoantipyrine Phenol o-dianisidine Per oxidase Glucose Acetic acid Nitric acid Anthrone Sulphuric acid Sodium sulphite 80% Ethanol 1-napthol Alcohol Perchloric acid Calcium chloride Silver nitrate Phenolphthalein indicator Orcinol Zinc dust Potassium hydroxide

40 41 42 43 44 45 46 47 48 49 50

G6125 SIGMA

Glucose Oxidase from Aspergillus niger

Type II, 15,000 units/g solid (without added oxygen) http://www.sigmaaldrich.com/catalog/product/sigma/g6125?lang=en&region=PK