APPLICATION FORM

YSP-Travel Fellowships for 13th FAOBMB Congress, Bangkok, November 23-29, 2012.
Name of Applicant: Dr. Yuzheng Zhao Position: Postdoctoral Fellow (indicate training/career stage) Postdoctoral Doctor Master Bachelor East China University of Science and Technology East China University of Science and Technology East China University of Science and Technology ShanDong University

07/2012-Now 09/2009-06/2012 09/2007-09/2009 09/2003-07/2007

Address of Applicant: (Normally this will be your institutional or office address) Room 733, The 18th research building, East China University of Science and Technology, Shanghai, P.R. China. 200237

Email address:
yuzhengzhao@ecust.edu.cn

Membership of FAOBMB Constituent Society: (Indicate the Constituent Society of FAOBMB in your country/region of residence which you are a member). Please see Societies Profile under About FAOBMB: Constituent Member on the webpage at: http://faobmb.net Chinese Society of Biochemistry and Molecular Biology Date of Birth: October 8, 1983 Applicants must be no more than 35 years of age at the closing date for application: (31 July, 2012). Proof of Age: (Certification by the Head of your Institution or Department is required) I certify on the basis of official documentation which I have seen (e.g. passport, birth certificate or drivers licence) that the date of birth of the applicant Dr. Yuzheng Zhao is 8/10/1983, as shown above. (DD/Month/Year) Page 1 of 7

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Curriculum Vitae Provide a brief CV showing education and research training, including any prizes or awards: (no more than one page)

Yuzheng Zhao
Emailyuzhengzhao@ecust.edu.cnMobile+86021-64251287 DegreeDoctor Research directionBiochemical and Cell Biology Date of birthOct.08,1983 AddressRoom733, The 18th Research Building , East China University of Science and Technology, Shanghai, P.R. China. 200237 Education: 07/2012-Now Postdoctoral East China University of Science and Technology 09/2009-06/2012 Doctor East China University of Science and Technology 09/2007-09/2009 Master East China University of Science and Technology 09/2003-07/2007 Bachelor Shandong University Awards and honors: 2012 Outstanding Graduates of Shanghai 2011 National Baosteel Excellent Student Award 2011 The top prize for the presentation entitled Genetically Encoded Fluorescent Sensors for Intracellular NADH Detection on the third Life Sciences Forum of Shanghai Institutions and Universities 2011 The presentation entitled Spatiotemporal detection of NADH based on fluorescent protein on the 7th Shanghai Cell Biology Youth Forum 2011 The Grand Prize on the 15th annual research forum of East China University of Science and Technology 2011 High-level paper award of East China University of Science and Technology Publications: 1. Y. Zhao, J. Jin, Q. Hu, H.M. Zhou, J. Yi, Z. Yu, L. Xu, X. Wang, Y. Yang, J. Loscalzo. Genetically encoded fluorescent sensors for intracellular NADH detection. Cell Metabolism14(4), 555-566(2011)(IF 18.207) 2. Y. Zhao, Y. Yang. Frex and FrexH: Indicators of metabolic states in living cells. Bioengineered Bugs 3(3), 183-190 (2012)(New journal) 3. Y. Yang, J. Jin, Y. Zhao, Q. Hu. Development and application of genetically encoded fluorescent sensors for Nicotinamide adenine dinucleotide.(Patent No. 201110288807.6)

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List of Publications in International Journals* * Number all publications in international journals or book chapters, with earliest first and most recent at the end. Provide the Impact Factor (IF) of the journal and indicate your contribution the work described and the publication. Use as many pages as required. Follow the style in these examples: 1. Y. Zhao, Y. Yang. Frex and FrexH: Indicators of metabolic states in living cells. Bioengineered Bugs 3(3),183-190 (2012)(New journal) Y. Zhaos contribution to this work was that Y. Zhao designed the experiments and interpreted results; Y. Zhao carried out all live cell research and imaging; Y. Zhao and Y. Yang wrote the manuscript. 2. Y. Zhao, J. Jin, Q. Hu, H.M. Zhou, J. Yi, Z. Yu, L. Xu, X. Wang, Y. Yang, J. Loscalzo. Genetically encoded fluorescent sensors for intracellular NADH detection. Cell Metabolism14(4), 555-566(2011)(IF 18.207) Y. Zhaos contribution to this work was that Y. Zhao and his partner designed the experiments and interpreted results; Y. Zhao and his partner carried out in vitro characterization; Y. Zhao carried out all live cell research and imaging; Y. Zhao and his partner wrote the manuscript. Y. Zhao and his partner have developed genetically encoded uorescent sensors for reduced nicotinamide adenine dinucleotide (NADH); Using these sensors, Y. Zhao firstly achieved the real-time detection and imaging of NADH in subcellular compartments. Y. Zhao measured subcellular NADH levels in mammalian cells. Y. Zhao demonstrate the utility of these sensors in mammalian cells by monitoring the dynamic changes in NADH levels in subcellular organelles as affected by NADH transport, glucose metabolism, mitochondrial electron transport chain function, and redox regulation.

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Abstract Indicate below the abstract that is submitted by you for presentation at the YSP and the FAOBMB Congress in Bangkok (include all authors, affiliation(s) and the text of the abstract) High spatiotemporal analysis of metabolic states in living cells
Yuzheng Zhao1, Jing Jin1, Qingxun Hu1, Hai-Meng Zhou2, Jing Yi3, Zhenhang Yu4, Lei Xu1,Xue Wang1, Yi Yang1,* and Joseph Loscalzo5 1Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, School of pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China 2Protein Science Laboratory of the Ministry of Education, School of Life Sciences, Tsinghua University, Beijing 100084, China 3Department of Biochemistry and Molecular Cell Biology, Key Laboratory of the Education Ministry for Cell Differentiation and Apoptosis, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, 280 South Chongqing Road, Shanghai 200025, China 4China National Center for Biotechnology Development, Beijing 100039, China 5Cardiovascular Division, Department of Medicine, Brigham and Womens Hospital, Harvard Medical School, Boston, MA 02115, USA

Reduced nicotinamide adenine dinucleotide (NADH) and its oxidized form, NAD+, play central roles in energy and redox metabolisms. For many years, researchers have relied on the weak NADH endogenous fluorescence signal to determine the NADH level in living cells. We recently reported a series of genetically encoded fluorescent sensors highly specific for NADH (Yuzheng Zhao et al, 2011 and 2012). Using these sensors, we firstly achieved the real-time detection and imaging of NADH in subcellular compartments. We measured subcellular NADH levels in mammalian cells through these tools and found extremely low cytosolic NADH levels ranging from 120 nM to 130 nM. However, the mitochondrial free NADH level is approximately 30 M. We demonstrate the utility of Frex in mammalian cells by monitoring the dynamic changes in NADH levels in subcellular organelles as affected by NADH transport, glucose metabolism, mitochondrial electron transport chain function, and redox regulation, and we demonstrate the temporal separation of changes in mitochondrial and cytosolic NADH levels with perturbation. These results support the view that cytosolic NADH is sensitive to environmental changes, while mitochondria have a strong tendency to maintain physiological NADH homeostasis. These sensors are also viable alternatives to existing techniques for measuring the endogenous fluorescence of intracellular NAD(P)H because of their superior sensitivity and specificity.

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Personal Statement Indicate briefly here your Research Interests and Career Goals, why you are interested to participate in the YSP Program (including what you will bring to the YSP and what you hope to gain from it): (no more than one page) Research Interests: Development and application of fluorescent sensors for tracking redox metabolism in mammalian cells. Career Goals: Looking for a full-time position in Research (Academic/Industrial), where I can demonstrate my technical and experimental skills and contribute to the development and better understanding of science for benefit. YSP is the stage of young scientists, where I not only can recognize many outstanding young scientists, will also greatly expand the international horizons, to learn advanced research ideas and research methods. I will bring the latest scientific advances in YSP Program, such as high spatial and temporal resolution detection of intracellular metabolism.

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Attachments: Letters of recommendation from two referees.

Submission methods: You can apply in either of two ways: by sending an email with scanned attachments to the Chair of the FAOBMB Fellowship Committee, Prof. Piamsook Pongsawasdi: Piamsook.P@Chula.ac.th or P.Piamsook@gmail.com This is the preferred method. If using this method, please assemble the Application Form and the Attachments into a single PDF file. by sending a hard copy of the application form and supporting documents to: Professor Piamsook Pongsawasdi, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.

Closing Date: Applications must be received by 31 July, 2012 (Bangkok time, GMT + 7 Hours). Applicants will be notified by email of the decision of the Committee by no later than 15 September, 2012.

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