MCQ

1. When the biological catalyst was first recognized? A. Early 1600s. B. late 1600s C. early 1700s D. late 1700s

2. Which experiment first revealed the existence of biological catalyst? A. digestion of meat by stomach secretion B. conversion of starch to sugar by saliva C. fermentation of sugar by yeast D. none of them.

3. Who was the discoverer of the function ability of enzyme without the presence of the cell? A. Louis Pasteur B. Eduard Buchner. C. Sumner D. None of them. 4. Pepsin, trypsin were crystallized by A. John Northrop B. Moses Kunitz C. Both of them D. Sumner 5. Enzyme- was named by A. Linus Pauling B. Haldane C. Kuhne D. William Jencks 6. Sumners postulation all enzymes are protein was widely accepted inA. 1927 B. 1930 C. 1830 D. 1929

7. Enzyme substrate complex was first proposed by A. Michael Polanyi, 1921 D. None of them. B. Wurtz, 1903 C. Charles-Adolph Wurtz, 1880

8. Enzyme - was written by A. Kuhne B. Kunitz C. Haldane D. Wurtz

9. Lock and Key model was proposed by Emile Fisher in A. 1890 B. 1891 C. 1892 D. 1894

10. Daniel Koshland gave which model? A. lock and key E. only B 11. Allosteric enzymes are regulated by the binding ofA. Allosteric modulators B. Allosteric effectors C. both of them D. regulators B. induced fit. C. sequential model D. both B & C

12. Fine tuning of metabolic pathways are done by A. Allosteric non covalent regulation B. Allosteric regulation C. Covalent modification D. Irreversible regulation

13. When does the enzyme call as heterotropic? A. same substrate and modulator C. different substrate and inhibitor 14. When the enzyme is homotropic? A. same substrate and inhibitor C. same substrate and modulator B. same substrate and product D. all of them B. different substrate and modulator D. none of them

15. What is the property of homotropic enzyme? A. B. C. D. same active site and regulatory site active site and regulatory site both are in different subunits modulator binds covalently none of them 16. Which kinetic behavior belongs to Allosteric enzymes?

A. follow Michaelis-Menten behavior fully B. show hyperbolic curve in V0 vs. S C. show sigmoid curve in V0 vs. S D. show parabolic curve in V0 vs. S 17. The substrate concentration giving half maximal velocity of reaction catalyzed by an allosteric enzyme is represented byA. km B. k0.5 C. S0.5 D. both B & C E. both A & C

18. Cooperative interactions between protein subunits are reflected byA. hyperbolic curve in V vs. S diagram B. sigmoid curve in V vs. S diagram C. linear relationship between V & S D. all of them 19. Which two models explain sigmoid kinetic behave? A. lock and key & induced fit model B. concerted model & sequential model C. MWC model & sequential model D. both B & C 20. When does the k0.5 become lower in case of heterotropic allosteric enzymes? A. when inhibitor binds to the enzyme B. when the substrate concentration rises C. when the activator binds to the enzyme D. all of them

ANSWER
1.D 2.A. 3.B 4.C 5.C 6.B 7.C 8.C 9.D 10.D 11.C 14. C 15.A 16.C 17.C 18.B 19.D 20.C 12.A 13.B