Bifidobacterium breve Reuter

ATCC Number
Designations

: 15700
: S1 (Variant a)

Source of Isolation Growth Conditions CM149)

: Intestine of infant : ATCC medium1053: Reinforced Clostridial medium (Oxoid Alternate medium 593: Chopped meat medium

Temperature Atmosphere Applications

: 37C : ***Anaerobic : Produces restriction endonuclease BbeSI

ATCC medium: 593 Chopped meat medium Ground beef (free of fat)........500.0 g Distilled water....................1.0 L N NaOH............................25.0 ml Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water, and NaOH and bring to a boil while stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1 liter original volume. To the filtrate add: Peptone...........................30.0 g Yeast extract......................5.0 g K2HPO4 .............................5.0 g 0.025% Resazurin solution..........4.0 ml Boil, cool and add 0.5 g L-cysteine . HCl; adjust pH to 7.0. Under 97% nitrogen, 3% hydrogen, dispense 7 ml over 1 part meat particles to 4-5 parts fluid per test tube. Cap with butyl rubber stoppers under N2, H2 and autoclave in press for 15 minutes under fast exhaust.

Citrobacter freundii (Braak) Werkman and Gillen

ATCC Number
Designations

: 8090
: [ATCC 13316, NCTC 9750]

Growth Conditions Temperature Atmosphere Applications

: ATCC medium3: : 37C : Aerobic

Nutrient agar or nutrient broth

: produces restriction endonuclease CfrAI quality control strain reference material quality control strain for Sensititre products

ATCC Medium: 3 Nutrient Agar/Broth Agar Medium Nutrient Agar (BD 213000)..23 g DI Water.1000 ml Autoclave at 121C. Broth Medium Nutrient Broth (BD cat 234000).8.0 g DI Water...1000 ml Autoclave at 121C. *This product is commercially available from BD. To make medium from scratch, follow formulation below: Nutrient Agar Composition Beef Extract....3.0 g Peptone...5.0 g Agar.15.0 g Final pH 6.8 +/- 0.2. *Omit agar for broth medium.

Enterococcus faecalis (Andrewes and Horder) and Schleifer andKilpper-Balz deposited as Streptococcus faecalis Andrewes and Horder ATCC Number
Designations

: 29212
: [Portland]

Source of Isolation Growth Conditions sheep blood heart infusion Temperature Atmosphere Applications

: Urine : ATCC medium260: Trypticase soy agar with defibrinated Alternate medium 44: Brain heart infusion agar or brain : 37C : Aerobic : control strain [21528] [21531] evaluation of Mueller-Hinton agar [21524] media testing [21529] [92284] [92325] [92390] [92429] [92819] [92844] [92845] quality control [92216] quality control strain [92138] [92149] [92159] [92160] susceptibility disc testing [21523] [92224] [92355] susceptibility testing [21525] [21533] [90283] [92217] [92353] [92422] [92423] [92424] [92425] [92788] quality control strain for API, BBL bioMerieux Vitek, IDS,
Micro-Media, MicroScan and Sensititre products

ATCC Medium: 260 Tryptic Soy Agar/Broth with 5% Sheep Blood (defibrinated) Agar Medium Tryptic Soy Agar (BD 236950)40 g Sheep Blood (defibrinated)..50 ml DI Water...950 ml Autoclave at 121C. Cool sterilized medium to ~47C. Aseptically add 50 ml of room temperature defibrinated sheep blood. Gently mix and dispense as required. Broth Medium Tryptic Soy Broth (BD 211825).30.0 g Sheep Blood (defibrinated)50 ml DI Water....950 ml Follow same procedure as agar medium above. If commercialized product is not available, Tryptic Soy Agar can be made from scratch, by following the formulation below:

Tryptic Soy Agar Composition Tryptone15.0 g Soytone.5.0 g NaCl...5.0 g Agar...15.0 g DI Water.950 ml pH to 7.3 +/- 0.2. (Tryptone-Pancreatic Digest of Casein; Soytone-Papaic Digest of Soybean Meal). To make Tryptic Soy Broth from scratch, follow Tryptic Soy Agar formulation but omit agar.
ATCC Medium: 44 Brain Heart Infusion Agar/Broth Agar Medium Brain Heart Infusion Agar (BD 211065)..52 g DI Water...1000 ml Autoclave at 121C. Broth Medium Brain Heart Infusion Broth (BD 237500)...37.0g DI Water.1000 ml Autoclave at 121C. *This product is commercially available from BD. To make medium from scratch, follow formulation below: Brain Heart Infusion Composition Calf Brains, infusion from..200 g Beef Hearts, infusion from.250 g Proteose Peptone...10.0 g Dextrose....2.0 g NaCl...5.0 g Na2HPO4......2.5 g DI Water.1000 ml Final pH 7.4 +/- 0.2 *Omit agar for broth medium

Lactobacillus gasseri Lauer and Kandler deposited as Lactobacillus acidophilus (Moro) Hansen and Mocquot ATCC Number
Designations

: 19992
: 1SL4 [F 164, VPI 6033]

Source of Isolation Growth Conditions Temperature

: feces : ATCC medium416: Lactobacilli MRS broth : 37.0C

ATCC Medium: 416 Lactobacilli MRS Broth Lactobacilli MRS Broth (BD 288130).55.0 g DI Water.....1000 ml Autoclave at 121C. *This product is commercially available from BD. To make medium from scratch, follow formulation below: Lactobacilli MRS Broth Composition Proteose Peptone #3.10.0 g Beef Extract.10.0 g Yeast Extract...5.0 g Dextrose..20.0 g Sorbitan Monooleate.1.0 g Ammonium Citrate.2.0 g Sodium Acetate..5.0 g MnSO4 x H2O.0.05 g Na2HPO42.0 g DI Water.. Final pH 6.5 +/- 0.2

1000 ml

Lactobacillus rhamnosus (Hansen) Collins et al. deposited as Lactobacillus casei (Orla-Jensen) Holland

ATCC Number

: 7469

Growth Conditions Designations NCDO 243,

: ATCC medium416: Lactobacilli MRS broth : [BUCSAV 227, M. Rogosa V300, M.E. Sharpe H2,

NCIB 6375, NCIB 8010, NCTC 6375, NRC 488, P.A. Hansen 300, R.P. Tittsler 300] Temperature Atmosphere Applications : 37C : ***5% CO2 : assay of folic acid assay of niacinamide nicotinamide assay of riboflavin vitamin B2 produces folate transport protein

ATCC Medium: 416 Lactobacilli MRS Broth Lactobacilli MRS Broth (BD 288130).55.0 g DI Water.....1000 ml Autoclave at 121C. *This product is commercially available from BD. To make medium from scratch, follow formulation below: Lactobacilli MRS Broth Composition Proteose Peptone #3.10.0 g Beef Extract.10.0 g Yeast Extract...5.0 g Dextrose..20.0 g Sorbitan Monooleate.1.0 g Ammonium Citrate.2.0 g Sodium Acetate..5.0 g MnSO4 x H2O.0.05 g Na2HPO42.0 g DI Water..1000 ml Final pH 6.5 +/- 0.2

Carnobacterium funditum Franzmann et al.

ATCC Number Designations Source of Isolation Growth Conditions Temperature

: 49836 : ACAM 312 [DSM 5970, Pf3] : Ace Lake, Vestfold Hills, Antarctica : ATCC medium1823: TSBY salt medium : 20.0C

ATCC medium: 1823 TSBY salt medium Trypticase Soy Broth (BD 211768)........30.0 g Yeast extract............................3.0 g NH4Cl ....................................0.25 g NaCl....................................13.0 g MgCl2 . 6H2O .............................4.0 g MgSO4 . 7H2O .............................3.45 g KCl......................................0.34 g CaCl2 . 2H2O .............................0.14 g Distilled water..........................1.0 L Adjust for final pH 7.2. Autoclave at 121C for 15 minutes.

Streptococcus salivarius subsp. thermophilus (Orla-Jensen) Farrow and Collins deposited as Streptococcus thermophilus Orla-Jensen
ATCC Number Designations CCUG : 19258 : NCDO 573 [NCIMB 8510, B of R, CNCTC 28/89, DSM 20617,

21957, CIP 102303, LMG 6896] Source of Isolation Growth Conditions infusion Temperature Atmosphere Applications : Pasteurized milk : ATCC medium260: Trypticase soy agar with defibrinated sheep blood Alternate medium 44: Brain heart infusion agar or brain heart : 37C : Aerobic : Produces aminopeptidase N Quality control strain

ATCC Medium: 260 Tryptic Soy Agar/Broth with 5% Sheep Blood (defibrinated)

Agar Medium Tryptic Soy Agar (BD 236950)40 g Sheep Blood (defibrinated)..50 ml DI Water...950 ml Autoclave at 121C. Cool sterilized medium to ~47C. Aseptically add 50 ml of room temperature defibrinated sheep blood. Gently mix and dispense as required. Broth Medium Tryptic Soy Broth (BD 211825).30.0 g Sheep Blood (defibrinated)50 ml DI Water....950 ml Follow same procedure as agar medium above. If commercialized product is not available, Tryptic Soy Agar can be made from scratch, by following the formulation below: Tryptic Soy Agar Composition Tryptone15.0 g Soytone.5.0 g NaCl...5.0 g Agar...15.0 g DI Water.950 ml pH to 7.3 +/- 0.2. (Tryptone-Pancreatic Digest of Casein; Soytone-Papaic Digest of Soybean Meal). To make Tryptic Soy Broth from scratch, follow Tryptic Soy Agar formulation but omit agar ATCC Medium: 44 Brain Heart Infusion Agar/Broth Agar Medium Brain Heart Infusion Agar (BD 211065)..52 g DI Water...1000 ml Autoclave at 121C. Broth Medium Brain Heart Infusion Broth (BD 237500)...37.0g DI Water.1000 ml Autoclave at 121C. *This product is commercially available from BD. To make medium from scratch, follow formulation below: Brain Heart Infusion Composition Calf Brains, infusion from..200 g Beef Hearts, infusion from.250 g Proteose Peptone...10.0 g Dextrose....2.0 g NaCl...5.0 g Na2HPO4......2.5 g DI Water.1000 ml Final pH 7.4 +/- 0.2