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Introduction: BIOCONVERSION
Sugarcane residue
What is Biomass
Living and dead biological material that can be used for biofuel or industrial production. Focus on biomass produced from agriculture activities.
Industrial chemicals (organic acids, acetic acids, giberellic acids, biopolymers) Food additives (amino acids, nucleosides, vitamins, fats and oils) Health care products (antibiotics, steroid, vaccines, monoclonal antibodies) Industrial enzymes (amylases, proteases, diastases).
Physical Method
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A process where heat is the dominant mechanism to convert biomass into another chemical form Three different classes of thermo chemical: Combustion/burning Gasification convert carbonaceous materials into carbon monoxide&hydrogen (syngas) Liquefaction
Biological methods
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use of the enzymes of bacteria and other microorganisms to break down biomass. micro-organisms are used to perform the conversion process: anaerobic digestion, fermentation and composting. The importance group of bacteria in bioconversion are: Lactic acid bacteria Acetic acid bacteria Bacteria of alkaline fermentation
What is bioconversion
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Bioconversion is the conversion of organic materials, such as plant or animal waste, into usable products or energy sources by biological processes or agents, such as certain microorganisms or enzymes. Things to consider: What to convert what to use What to get
Bioconversion can be carried out physically, thermochemically and biologically. This process has been applied in the production of foodstuffs, organic chemicals and energy. Biological methods for bioconversion has given priority with the use of microorganisms as less expensive yet effective agents. This process is also known as fermentation.
Types of Bioconversion
Solid state fermentation process can be defined as the growth of microorganisms especially fungus on insoluble substrate with sufficient moisture but not free water. On the contrary, in liquid state fermentation, microorganisms are grown in liquid media with existence of water.
Heat sterilization and aseptic control High volumes of water consumed and effluent discarded Easy control of temperature Limitation of soluble oxygen, high level of air required Easy pH control Good homogenization
Scale up Inoculation
Need for engineering and new design equipment Spore inoculation, batch, high inoculum volume needed, spore have longer lag time due to the need for germination
Risk of contamination for low rate growth fungi Low energy consuming Low volumes and low cost of equipments
Risks of contamination for single strain bacteria High energy consuming High volumes and high cost technology
Acetic acid
CH3COOH, also known as ethanoic acid is an organic acid that gives vinegar its sour taste and pungent smell. Acetic acid is one of the simplest carboxylic acids. Usage : - in vinegar making (4%-18% acetic acid) - solvent - cellulose acetate used in photographic film
is a genus of acetic acid bacteria have the ability to convert ethanol to acetic acid in the presence of oxygen They are Gram-negative, aerobic rod-shaped bacteria.
Type of culture : highly aerated fermentation Raw material : diluted purified ethanol from grape juice, apple juice, barley malt etc. Acetic acid fermentation : - Acetobacter convert alcohol to acetic acid in the presence of excess oxygen. - The oxidation of one mole of ethanol yields one mole each of acetic acid and water; - C2H5OH + O2 CH3COOH + H2O
the efficiency depends on the ratio between the energy input necessary per unit weight of O2 transferred to the culture.
2. Over-oxidation
when there is over-oxidation, acetic acid will convert to CO2 and H2O. will decrease acetic acid production. have to maintain acetic acid concentrations above 6% of the total culture. and avoid the total depletion of ethanol.
Citric acid
is a weak organic acid C6H8O7 exists in greater than trace amounts in a variety of fruits and vegetables, most notably citrus fruits commercial citric acid is produced by fermentation of carbohydrates or citrus juices Usage : - to add an acidic or sour taste to foods and soft drinks. - general additive in the confectionery industry. - pharmaceutical industries
Microorganism used : Aspergillus niger or Candida sp. (yeast) Culture method : submerged fermentation system and surface fermentation Raw materials : Molasses, sugarcane syrup, sucrose
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Breakdown of hexoses (sugar) to pyruvate and acetyl CoA. The anaplerotic formation of oxaloacetate from pyruvate and CO2 The accumulation of citrate within the tricarboxylic acid cycle
sensitive to iron. Medium used must be iron-deficient. Fermentor must be stainless steel to prevent leaching of iron frm fermentor wall Oxygen supply pH should maintain below 2.0. At higher values, A.niger accumulates gluconic acid rather than citrate.
Ethanol production
Ethanol or ethyl alcohol (C2H5OH) is a clear colourless liquid, it is biodegradable, low in toxicity and causes little environmental pollution if spilt. Ethanol burns to produce carbon dioxide and water. Ethanol is widely used in Brazil and in the United States. Most cars on the road today in the U.S. can run on blends of up to 10% ethanol and 90% petrol Application of ethanol : raw material, solvent, used in fuel and in chemical, pharmaceutical & food industries.
Bioethanol, unlike petroleum, is a form of renewable energy that can be produced from agricultural feedstocks. It can be made from very common crops such as sugar cane, potato, manioc and maize.
- For commercialization of ethanol production, two different types of substrates are available for fermentation. - Both substrates need different type of pre-treatment. 1. Sugar containing biomass 2. Starch containing biomass
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Sugar containing biomass : sugar cane, molasses, sugar beet Production steps : 1. milling/grinding (extract juices) 2. fermentation of juices (sugar) with yeast
sugar + yeast C6H12O6 + yeast ethanol + carbon dioxide 2C2H5OH + 2CO2
3. Distillation 4. Dehydration
Starch containing biomass : maize, cassava, grain, potato Production steps : 1.Slurry preparation The starch-containing substrate (Cassava powder) is mixed with water to form slurry. 2.Gelatinization The slurry is then gelatinized with steam (68-74C). Gelatinization is the formation of starch paste.
3.Dextrinization Dextrinization is the breakdown of gelatinized starch into smaller fragments or dextrins by means of - or -amylase. The action of -amylase on gelatinized starch results in dramatic reduction of viscosity. 4.Saccharification Saccharification is the complete conversion of dextrins into glucose (sugar) through the action of glucoamylase. 5.Fermentation The resulting sugar is cooled and transferred to a fermentor where yeast is added. It is catalyzed by the action of enzymes present in microorganisms like yeasts with ethyl alcohol as the end product.
6.Distillation After fermentation, the fermented liquor is transferred to a distillation process where the ethanol is separated from the remaining stillage (residue non-fermentable solids and water). Distillation is the process in which a liquid or vapor mixture of two or more substances is separated into its component fractions of desired purity by the application or removal of heat. This process can usually produce a 95.6% by volume ethanol product. 7.Dehydration Ethanol from distillation process is sent to the molecular sieves column for further dehydration to produce 99.7% v/v ethanol.
cellulose containing biomass : paddy straw, wood, coconut husk, paper waste Production steps : 1. biomass harvested 2. biomass pretreatment with heat or chemicals (NaOH, HCL) Cellulose is a polymer of glucose. Hemicellulose is a copolymer of different C5 and C6 sugars including e.g. xylose, mannose and glucose, depending on the type of biomass. Lignin is a branched polymer of aromatic compounds.
3. Hydrolysis of cellulose with enzyme nto produce sugar 4. Fermentation of sugar with yeast
ethanol + carbon dioxide 2C2H5OH + 2CO2
5. Distillation After fermentation, the fermented liquor is transferred to a distillation process where the ethanol is separated from the remaining stillage (residue nonfermentable solids and water).
Biodiesel production
Biodiesel
Biodiesel refers to a vegetable oil- or animal fatbased diesel fuel consisting of long-chain alkyl (methyl, propyl or ethyl) esters. Biodiesel is typically made by chemically reacting lipids (e.g., vegetable oil, animal fat, soybean, palm oil, jathropa, sunflower oil, canola) with an alcohol. Biodiesel can be used in pure form or may be blended with petroleum diesel at any concentration in most injection pump diesel engines.
Biodiesel is a light to dark yellow liquid. It is practically immiscible with water, has a high boiling point and low vapor pressure. Biodiesel is a renewable fuel that can be manufactured from algae, vegetable oils, animal fats or recycled restaurant greases; it can be produced locally in most countries. It is safe, biodegradable and reduces air pollutants, such as particulates, carbon monoxide and hydrocarbons. Blends of 20 percent biodiesel with 80 percent petroleum diesel (B20) can generally be used in unmodified diesel engines. Biodiesel can also be used in its pure form (B100), but may require certain engine modifications to avoid maintenance and performance problems.
Biodiesel production
Biodiesel production is the act of producing the biodiesel, through either transesterification or alcoholysis. The process involves reacting vegetable oils or animal fats catalytically with a short-chain aliphatic alcohols (typically methanol or ethanol).
Production steps : biodiesel from soybean seeds 1. Raw materials screening Remove impurities/dirts from raw materials 2. Oil extraction Extract oil by pressing or using solvent extraction 3. Purification Remove impurities from the oil (centrifuge) 4. transesterification Reaction of oil with methanol+catalyst (NaOH, HCl, lipase)+heat. Will produce methyl ester and Glycerol
Transesterification
5. Purification a) Separation of methyl ester with glycerine. Glycerine more dense than methyl ester. So glycerine will settle at the bottom. b)Wash biodiesel with water to remove contaminants. Water is heavier than biodiesel and absorb excess methanol+NaOH
Advantages of bioconversion
Increase recycling -generate money from waste Generation of renewable energy -bioethanol..biodiesel..biogas -not too dependent on fossil fuel Reduce landfill effect - It saves space in landfills. Offset to fossil fuel usage -expand energy freedom of choice. Reduce carbon emission -reduce greenhouse gasses by using bioenergy
Remediate ecological disaster -Municipal solid wastes is getting out of control necessitating bigger landfills that are further away from our urban centers. This excess waste contributes to land, water, and air pollution
Please read article entitle Carbons New Math to get full picture on this Advantages.
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Municipal solid wastes is getting out of control necessitating bigger landfills that are further away from our urban centers. This excess waste contributes to land, water, and air pollution Rural agricultural residues and damaged crops could have a higher value as soil amendments and biomass feedstock