1 - Bricker SB, Clement CG, Pirhalla DE, Orlando SP, Farrow DRG. 1999.

National Estuarine Eutrophication Assessment: Effects of Nutrient Enrichment in the Nations Estuaries. Silver Spring (MD): NOAA National Ocean Service Special Project Office and the National Centers for Coastal Oceal Science 2 - Wasmund, N. & Uhlig, S. 2003. Phytoplankton trends in the Baltic Sea. ICES J. Mar. Sci., 60, 177-186 2 Rabalais N., Turner R.E., Scavia D. (2002) Beyond Science into Policy: Gulf of Mexico Hypoxia and the Mississippi River. Bioscience, 52 (2), 129-142 3 - Vollenweider, R. A., Marchetti, R. & Viviani, R. 1992. Marine Coastal Eutrophication. Elsevier,London 3 - Nixon, S. W. 1995. Coastal marine eutrophication: a definition, social causes, and future concerns. Ophelia, 41, 199-219 3 - Cloern, J. E. 2001. Our evolving conceptual model of the coastal eutrophication problem. Mar. Ecol. Progr. Ser., 210, 223-253
4

Nixdorf, B., Rektins, A., Mischke, U. 2008. Standards and thresholds of the EU

Water Framework Directive (WFD): phytoplankton and lakes / Nixdorf, B., Rektins, A., Mischke, U. Standards and thresholds for impact assessment. p. 301-314 5 - Heiskanen, A.-S., van de Bund, W., Cardoso, A.C., Nges, P. 2004. Towards good ecological status of surface waters in Europe - interpretation and harmonisation of the concept. Water Science and Technology. 49 (7): 169177. 6 - Domingues RB, Barbosa A., Galvo H. (2008). Constrains on the use of phytoplankton as a biological quality element within the Water Framework Directive in Portuguese waters. Marine Pollution Bulletin, 56: 1389-1395 Moita, M.T., M.M. Martins & J.G. Ferreira (1984). A regression model for chlorophyll a in the Tejo Estuary. Separata do Cuadernos da rea de Ciencias Marias, Seminrio de Estudos Galegos 1:151-162 Cabeadas, L. (1999a). Phytoplankton production in the Tagus estuary (Portugal). Oceanol. Acta 22 (2): 205-214. Gameiro, C., P. Cartaxana, M.T. Cabrita & V. Brotas (2004). Variability in chlorophyll and phytoplankton composition in a estuarine system. Hydrobiologia 525: 113-124. Brogueira, M.J., M.R. Oliveira & G. Cabeadas (2007). Phytoplankton community

structure defined by key environmental variables in Tagus estuary, Portugal. Marine Environmental Research 64: 616628 Bettencourt A., F.V. Gomes, J.A. Dias, J.G. Ferreira, M.C. Silva & J.L. Costa (2003). Esturios portugueses. INAG Lisboa. Quadrado, F. & F. Gomes (1998) Modelao da qualidade da gua no rio Tejo. Conference on Application of Models in Water management Amesterdam. Grasshoff, K. (1976). Methods of seawater analysis. Verlag. Chimie, New York. Bendschneider, K. & N. J. Robison (1952). A new spectrophotometric determination of nitrite in seawater. J. Mar. Res. 11:87-96. Murphy, J. & J.P. Riley, (1962). A modified single solution method for the determination of phosphate in natural waters. Chim. Acta 27. 301-318. Fanning, J. C. & M. E. Q. Pilson (1973). On the spectrophotometric determination of dissolved silica in natural waters. Analyt. Chem. 45, 136-140. Koroleff, F. (1969/70). Direct determination of ammonia in natural waters as indophenol blue. Int. Counc. Explor. Sea (ICES). Pap. C.M. 1969/ C: 9, revised 1970, pp. 19-22.
Vollenwieder, R. A. (1974). A manual on methods for measuring primary production in aquatic environments. IBP Handbook R. London, Blackwell Scientific Publication, 225 pp.

Hillebrand, H., Durselen, C.-D., Kirschtel, D., 1999. Biovolume calculation for pelagic and benthic microalgae. Journal of Phycology 35, 403e424. Rocha, O., Duncan, A., 1985. The relationship between cell carbon and cell volume in freshwater algal species used in zooplankton studies. Journal of Plankton Research 7: 279.294. Brotas V. & M. R. Plante-Cuny (1996). Identification et quantification des pigments chlorophylliens et carotnodes des sdiments marins. Choix dun protocole danalyse par HPLC. Oceanologica Acta, 19(6): 623-633. Kraay G.W., M. Zapata and M.J.W. Veldhuis (1992). Separation of Chlorophylls c1, c2, and c3 of marine phytoplankton by reversed-phase-C18-high-performance liquid

chromatography. J. Phycol 28 (1992), pp. 708712. Bolhar-Nordenkampf H. R., S. P. Long, N. R. Baker, G. Oquist, U. Schreiber, E. G. Lechner (1989). Chlorophyll Fluorescence as a Probe of the Photosynthetic Competence of Leaves in the Field: A Review of Current Instrumentation. Functional Ecology, Vol. 3, No. 4, pp. 497-514. Kautsky, H., Hirsch, A. (1931), Neue Versuche zur Kohlensureassimilation, Naturwissenschaften, 19:964-964. Kitajima M, Butler WL. Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone. Biochim Biophys Acta. 1975 Jan 31;376(1):105115. Gameiro, C., P. Cartaxana & V. Brotas, (2007). Environmental drivers of phytoplankton distribution and composition in Tagus estuary, Portugal, Estuarine, Coastal and Shelf Science 75 (12) (2007), pp. 2134. Carrick, H.J. and C.L. Schelske, 1997. Have we overlooked the importance of small phytoplankton in productive waters? Limnol. Oceanogr. 42: 16131621. Descy J. P., H.W. Higgins, D. J. Mackey, J. P. Hurley & T. M. Frost, 2000. Pigment ratios and phytoplankton assessment in northern Wisconsin lakes. J. Phycol. 36: 274 286. Mackey, D. J., H. W. Higgins, M. D. Mackey & D. Holdsworth, 1998. Algal class abundance in the western equatorial Pacific: Estimation from HPLC measurements of chloroplast pigments using CHEMTAX. Deep Sea Res. I 45: 14411468. Schlter, L., F. Mhlenberg, H. Havskum & S. Larsen, 2000. The use of phytoplankton pigments for identifying and quantifying phytoplankton groups in coastal areas: testing the influence of light and nutrients on pigment/chlorophyll a ratios. Mar. Ecol. Prog. Ser. 192: 4963. Marinho M.M., Rodrigues S.V. (2003) Phytoplankton of an eutrophic tropical reservoir: comparison of biomass estimated from counts with chlorophyll-a biomass from HPLC measurements. Hydrobiologia 505: 7788, 2003. McCormick, P.V., P.S. Rawlik, K. Lurding, E.P. Smith and F.H. Sklar, 1996. Periphytonwater quality relationships along a nutrient gradient in the northern Florida Everglades. J. N. Am. Benthol. Soc. 15: 433449. Cabrita M. T. & M. T. Moita (1995) Spatial and temporal variation of physico-

chemical conditions and phytoplankton during a dry year in the Tagus estuary (Portugal) Netherlands Journal of Aquatic Ecology 29: 323-332. Lippemeier S., Hintze R., Vanselow K.H., Hartig P. and Colijn F. (2001). In-line recording of PAM fluorescence of phytoplankton cultures as a new tool for studying effects of fluctuating nutrient supply on photosynthesis. Eur. J. Phycol. 36: 89-100.

Belshe E.F., Durako M.J., Blum J.E. (2007). Photosynthetic rapid light curves (RLC) of Thalassia testudinum exhibit diurnal variation. Journal of Experimental Marine Biology and Ecology 342: 253268

Rottgers R. (2007). Instruments and Methods-Comparison of different variable chlorophyll a fluorescence techniques to determine photosynthetic parameters of natural phytoplankton. Deep-Sea Research I 54 437451 Serdio J., Cruz S., Vieira S., Coelho H. (2006) Rapid light-response curves of chlorophyll fluorescence in microalgae: relationship to steady-state light curves and non-photochemical quenching in benthic diatom-dominated assemblages. Photosynthesis Research. 90, 29-43 Kromkamp J. & J. Peene (1999). Estimation of phytoplankton photosynthesis and nutrient limitation in the Eastern Scheldt estuary using variable fluorescence. Aquatic Ecology 33: 101104. Sharp, J.H., 2001. Marine and aquatic communities, stress from eutrophication. In: Levin, S. (Ed.), Encyclopedia of Biodiversity, vol. 4. Academic Press, San Diego, CA, USA, pp. 1e11. Yoshiyama, K., Sharp, J.H., 2006. Phytoplankton response to nutrient enrichment in an urbanized estuary: apparent inhibition of primary production by overeutrophication. Limnology and Oceanography 51, 424e434. Jesus B., V. Brotas, M. Marani & D.M. Paterson (2005). Spatial dynamic of microphytobenthos determined by PAM fluorescence. Estuarine, Coastal and Shelf Science, 65: 30-42. Wirtz K. , K. Wiltshire (2005). Long-term shifts in marine ecosystem functioning detected by inverse modeling of the Helgoland Roads time-series. Journal of Marine Systems, Volume 56, Issue 3-4, Pages 262-282. Lorenzen, C.J. (1967). Determination of chlorophyll and phaeopigments: spectrophotometric equations. Limnology & Oceanography 12: 343-346.