The Solution is Dilution 5/25/12 Emily Stout

Purpose/Introduction In this lab, students will be able to properly dilute prepare solutions of different concentrations and molarities. If we add more of the stock solution to the diluted solution, the color will be more prominent. Pre-lab Questions 1. 342g of sucrose 2. 8.55g of sucrose 3. 5mL of sucrose solution 4. 1.5mL of sucrose solution 5. The pipet is calibrated to have a little extra liquid left in it to allow for accurate measurement. Materials 1. Balance 2. Filter Papers 3. Volumetric Flask 4. Deionized Water 5. 5 Test Tubes 6. Wash Bottle 7. 10mL Graduated Cylinder 8. Pipet 9. Food Coloring 10. 11. 12. Beaker Sucrose Test Tube Rack

13.

Mohr Pipet

Procedure Part A: Using the calculations from number two in the Pre-lab questions weigh out that much sucrose and transfer all of the sold into a clean volumetric flask. Then add deionized water until the flask is about twothirds full. Cap the flask with a cork and gently invert it 3-4 times to mix the solid. After inverting, continue to slowly fill the flask to the etched line with deionized water. When finished, invert the flask 7-10 times to dissolve the solid and add 3-5 drops of food coloring to the solution. Cap the solution to prevention and evaporation or contamination. This is the .10 M sucrose stock solution needed for the next part. Part B: Collect and place five labeled test tubes in the test tube rack. Next fill a 250mL beaker about half full with deionized water. You will use this water to rinse your pipet to avoid any contamination. Pour your stock solution into a 10mL graduated cylinder, it should be exactly 10mL, and then transfer it into the first test tube. When finished rinse your graduated cylinder and invert it to remove all water. Rinse all utensils. Using a clean 1mL pipet transfer one 1mL of the sucrose solution into the 10mL graduated cylinder and fill with deionized so that the final volume is 10mL. Then transfer the solution into the second test tube. Rinse all materials. Next add 1mL of the solution in test tube #2, transfer the solution into the graduated cylinder and fill so that the final volume is 10mL with ionized water. Rinse all utensils. Repeat the dilution process for the remaining test tubes. When all test tubes are filled, compare the color intensity and rank them 1-5. Rinse all materials. It is safe to pour the sucrose waste down the drain. Part C: Place your test tubes in the test tube rack and , using the 10mL graduated cylinder, pour 10mL of stock solution into test tube #1. Using a clean Mohr pipet, add 3.8mL of the sucrose stock solution cylinder to the graduated and fill to the 10mL mark with deionized water. Transfer the solution to test tube #2. Record all necessary data in to the chart. Again, using the clean Mohr pipet transfer 2.4mL of the stock solution into the graduated cylinder and fill to the 10mL mark with deionized water. Transfer this solution into test tube #3 and record all necessary data. Rinse all materials. Next, using the calculations you obtained from pre-lab question #3, prepare a 10mL solution of a .050 molarity of the sucrose solution by dilution of the stock solution. Record any necessary information and rinse all

materials. After you rinse your materials you need to use the calculations you obtained from pre-lab question #4 prepare a 10mL solution with a .0150 molarity by the process of dilution of the stock solution. Transfer this solution into test tube #5 and record any necessary data. When finished compare the color intensity of the solutions and rank them 1-5. After cleaning the data table, rinse and clean all materials.

Observations: In this lab we noticed that the sugar collected in the neck of the volumetric flask and it had to be inverted more than required. We also noted that the directions confused us a lot when it came to the data chart.

Data/Calculations:

Part B: Test Tube #1 Volume (in mL) of Concentrat ed Solution added (V #1) Concentrat ion of Concentrat ed Solution (M #1) Volume (in mL) of dilute solution (V #2) Concentrat ion of dilute solution (M 10 mL Test Tube #2 1mL Test Tube #3 1 mL Test Tube #4 1 mL Test Tube #4 1 mL

.1 M

.01 M

.001 M

.0001 M

.00001 M

10 mL

10 mL

10 mL

10 mL

10 mL

.1

.01

.001

.0001

.00001

#2) Color observatio ns 1-5 Lightest = 1 Darkest = 5 5 4 3 2 1

Part C: Test Tube #1 Volume (in mL) of Concentrat ed Solution added (V #1) Concentrat ion of Concentrat ed Solution (M #1) Volume (in mL) of dilute solution (V #2) Concentrat ion of dilute solution (M #2) Color Observatio 10 mL Test Tube #2 3.8 mL Test Tube #3 2.4 mL Test Tube #4 5 mL Test Tube #5 1.5 mL

.10 M

.38 M

.24 M

.5 M

.15 M

10 mL

10 mL

10 mL

10 mL

10 mL

.10 M

.144 M

.0567 M

.25 M

.0225 M

ns 1-5 Lightest = 1 Darkest = 5 *All calculations are potentially in the calculator, and there is no hope of finding them. Post Lab Questions/ Advanced Questions: 1. The higher the concentration, the higher the intensity of the color. 2. 10.375 grams of KI 3. .0025 M 4. .0625 M 5. To prepare a 500mL solution of 3 molarity sucrose solution you would you would first need to find the amount of moles of sucrose and convert 500ml to liters. x/.5 L = 3M x= 1.5 moles of sucrose And after that you need to use the molar mass of sucrose (342 g) and multiply it by 1.5 moles. After than you need to weigh out that amount on a scale and pour it into a 500mL volumetric flask. Fill the flask about three thirds full with deionized water and invert several times. When finished continue to fill it close to the line with a pipet. 6. To prepare a 100ml solution of .5 M HCl from a stock solution of HCl that has a molarity of 12.1, you would need to draw 10mL of the 12.1M solution and place it into the flask with a pipet. Fill the flask with deionized water and invert gently. Conclusion: In this lab we explored the process of dilution. We learned that the more of the stock solution you added to your new solution the more intense the color would be.