Escolar Documentos
Profissional Documentos
Cultura Documentos
Jaymie M. Rivera Gutirrez Dr. Claudia Ospina1, Dr. Mayra Pagn1 and Prof. Augusto Carvajal2
1Chemistry
Department University of Puerto Rico at Cayey 2Biology Department, University of Puerto Rico at Cayey
Aims:
{
Main:
z
The development and application of bioassay for the research of antitumor activity on tropical plants extract.
Specifics:
z z
Studying cytotoxic properties of tropical species. Chemotaxonomic study of tropical plants in Puerto Rico
Introduction
Since ancient times, in Puerto Rico some plants have been used to treat diseases like colds, coughs, diarrhea, respiratory infections, skin lesions and others. Unfortunately, it is estimated that only a few group of endemic and native plants have been studied around entire world. In Puerto Rico, we are at risk of losing the ancestral knowledge of the healing properties of plants.
Melndez, 2006
O O
O O
P o d o p h y ll o t o x in L ig n a n is o la t e d f r o m P o d o p h y llu m p e lta tu m
E t o p o s id e S y n th e tic D r u g
N O N N O OH O OH N O O HO N O
C a m p t o t h e c in A lk a lo id e x t r a c te d fr o m C a m p o th e c a a c u m in a ta
T o p o te c a n S y n th e tic D r u g
Srivastava, 2005
Goetzea Elegans (G.E. - endemic) Has not been reported any healing power yet.
Alain, 2006
February 23, 2008: A collection expedition was organized taking in consideration the distribution and abundance of the organisms in a specific area
Pimenta Racemosa
H exan e
C h lo r o fo r m
E th y l A c e ta te
B u t a n o l*
W a te r
* S o m e t im e s , b u t a n o l e x t r a c t io n is r e q u ir e d
GENERAL STRATEGY:
Methodology
{
Bioassay
Three of the six extracts were active with a LC50 200 g/mL. These extracts were obtained after partition with solvents of different polarities. The crude extracts of these species were inactive with a LC50 200 g/mL.
The functional groups for the crude extract of P.R. are aliphatic carbons under the 1-2 ppm region, aromatics in 67.1ppm, and double bonds at 5pmm. The high peaks under 3.5-4.8ppm is the methanol-d used as solvent.
The corresponding compound for this NMR is eugenol and the corresponding molecule is:
The functional groups for the crude extract of G.E. are aliphatic protons under the 0.6-2.4 ppm region, and double bonds at 5pmm.
TLC
TLC analysis: B C
Better mixture of solvents to purify the compounds 9.0mL Hexane with 1.0mL AcoEt
Chemical analysis:
z
Separation by column:
{
We made a separation by columns using the best mixture of solvents with different polarities based on the results of the TLC.
TLC analysis: B C D
Mixture of solvents :9.5mL Hexane with 0.5mL AcoEt
The possible functional groups for the hexane extract of G.E. are aliphatic carbons under the 1-2 ppm region, aromatics in 7-8ppm, and double bonds at 5pmm.
Future Work:
{
Extracts purification and separation will be based on the bioactivity results. NMR for the third fraction of G.E. hexane extract.
{ 13C
Acknowledgments:
Dr. Claudia Ospina { Isamar Ortiz, Karla Claudio and Janibeth Hernandez { Biominds Program { Chemistry and Technicians Department { Interdisciplinary Investigation Institute { RISE Program
{
Chemical Analysis and Biological Evaluation of Tropical Plants: Goetzea elegans and Pimenta racemosa
Jaymie M. Rivera Gutirrez Dr. Claudia Ospina1, Dr. Mayra Pagn1 and Prof. Augusto Carvajal2
1Chemistry
Department University of Puerto Rico at Cayey 2Biology Department, University of Puerto Rico at Cayey