Table of contents

Introduction The History and Influence of the ICPD Thomas H. Herdt Section A. Transition cow biology and management Advances in transition cow biology: new frontiers in production diseases J. K. Drackley Research priorities from a producers point of view Walter M. Guterbock Metabolic profiling to assess health status of transition dairy cows Robert J. Van Saun A consideration about the energy supply in peripartum of dairy cows on the basis of change in plasma free amino acid concentration S. Kawamura., K. Shibaro., R. Hakamada., M. Otsuka, S. Sat and H. Hoshi Metabolic predictors of displaced abomasum in transition dairy cows S. LeBlanc, T. Duffield and K. Leslie Evaluation of a rapid test for NEFA in bovine serum L. Gooijer, K. Leslie, S. LeBlanc and T. Duffield Association of rump fat thickness and plasma NEFA concentration with postpartum metabolic diseases in Holstein cows N.P. Joshi, T.H. Herdt and L. Neuder Effect of pre-partum feeding intensity on postpartum energy status of Estonian Holstein cows H. Jaakson, K. Ling, H. Kaldmae, J. Samartel, T. Kaart and O. Krt Using a pooled sample technique for herd metabolic profile screening Robert J. Van Saun Section B. Metabolic effects of immune mediators Metabolic effects of immune mediators Kirk C. Klasing The effect of bovine respiratory disease on carcass traits R.L. Larson 50 51 24 35 41 7 19

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Does calf health during the feedlot period affect gain and carcass traits? L.R. Corah, W.D. Busby, P. Beedle, D. Strohbehn, J.F. Stika Effects of dexamethasone on mRNA levels and binding sites of hepatic -adrenergic receptors in neonatal calves and dependence on colostrum feeding H.M. Hammon, J. Carron, C. Morel, and J.W. Blum Pathogenesis of avian growth plate dyschondroplasia N.C. Rath, J.M. Balog, G.R. Huff, and W.E. Huff Concentrations of haptoglobin and fibrinogen during the first ten days after calving in dairy cows with acute endometritis M. Drillich, D. Voigt, D. Forderung and W. Heuwieser Extracellular pH alters innate immunity by decreasing the production of reactive oxygen and nitrogen species, but enhancing phagocytosis in bovine neutrophils and monocytes D. Donovan High nitrite/nitrate status in neonatal calves is associated with increased plasma levels of S-nitrosoalbumin and other S-nitrosothiols I. Cattin, S. Christen, S.G. Shaw and J.W. Blum Effects of 0.03% dietary -glucans on nonspecific/specific immunity, oxidative/ antioxidative status and growth performance in weanling pigs S. Schmitz, S. Hiss and Helga Sauerwein Influence of organic nutrition and housing on selected immunological and metabolic properties in fattening pigs S. Millet, E. Cox, M. Van Paemel, B.M. Goddeeris and G.P.J. Janssens Section C. Animal behaviour and welfare in intensive production systems

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Associations of cow comfort indices with total lying time, stall standing time, and lameness 64 K.V. Nordlund, N.B. Cook and T.B. Bennett Interaction of lameness with sand and mattress surfaces in dairy cow freestalls K.V. Nordlund, N.B. Cook and T.B. Bennett Electric and magnetic fields (EMF) affect milk production and bevavior of cows D. Hillman, C.L. Goeke, D. Stetzer, K. Mathson, M.H. Graham, H.H. VanHorn, C.J. Wilcox 66 68

Effect of roughages added to the milk replacer diet of veal calves on behavior and gastric development 69 A.M. van Vuuren, N. Stockhofe, J.J. Heeres-van der Tol, L.F.M. Heutink and C.G. van Reenen Transportation stress of cattle by railway Z. Cui, B. Han, G. Gao and D. Cui 71

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Section D. Infection and infectious diseases associated with production systems Postweaning Multisystemic Wasting Syndrome (PMWS) in pigs Matti Kiupel Influence of growth conditions on caprine nasal bacterial flora in the presence of Mannhemia hameolytica D.R. McWhinney and Kingsley Dunkley The relationship between exposure to bovine viral diarrhea virus and fertility in a commercial dairy herd W. Raphael, S. Bolin, W. Guterbock, J. Kaneene, and D. Grooms Tritrichomonas foetus cysteine protease cp30 induces cell death in host cells B.N. Singh, J.J. Lucas, G.R. Hayes, C.E. Costello, U. Sommer and R.O. Gilbert Immunological approaches to enhanced production in food animals R.J. Yancey, Jr. Colostrum management in calves: effects of drenching versus bottle feeding M. Kaske, A. Werner, H.-J. Schubert, H.W. Kehler and J. Rehage Immune system activation increases the tryptophan requirement in post weaning pigs Nathalie Le Floch and Bernard Sve Periparturient negative energy balance and neutrophil function suppression are associated with uterine health disorders and fever in Holstein cows D.S. Hammon, I.M. Evjen, J.P. Goff, T.R. Dhiman Activities of enzymes in peripheral leukocytes reflect metabolic conditions in fattening steers T. Arai, A. Inoue, A. Takeguchi, S. Urabe, T. Sako, I. Yoshimura and N. Kimura Effects of 0.3% dietary -glucan on nonspecific/specific immunity, oxidative/ antioxidative status and growth performance in weanling pigs S. Schmitz, S. Hiss and H. Sauerwein Evaluation of Liver Abscess Incidence in Feedlot Cattle Fed a Dietary Antioxidant (AGRADO) across Four Studies M. Vzquez-An, F. Scott, B. Miller and T. Peters Pathogenic Escherichia coli in dairy cows held in farms with organic production (OP) and with integrated production (IP) P. Kuhnert, C. Dubosson, M. Roesch, E. Homfeld, M.G. Doherr and J.W. Blum 74

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Section E. Reproductive health Rearing conditions and disease influencing the reproductive performance of swedish dairy heifers J. Hultgren Factors influencing conception rate after synchronization of ovulation and timed artificial insemination B.-A. Tenhagen, C. Ruebesam and W. Heuwieser The incidence of endometritis and its effect on reproductive performance of dairy cows R.O. Gilbert, S.T. Shin, M. Frajblat, C.L. Guard, H.N. Erb and H. Roman Use of Neospora caninum vaccine in a dairy herd undergoing an abortion outbreak R.W. Meiring and P.J. Rajala-Schultz Ovulatory cycles, metabolic profiles, body condition scores and their relation to fertility of multiparous Holstein dairy cows J. Samartel, K. Ling, A. Waldmann, H. Jaakson, A. Leesme and T. Kaart 112

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Field trial on blood metabolites, body condition score (BCS) and their relation to the recurrence of ovarian cyclicity in Estonian Holstein cows 119 Katri Ling, Andres Waldmann, Jaak Samartel, Hanno Jaakson, Tanel Kaart, Andres Leesme In vitro embryo production: growth performance, feed efficiency, health status, and hematological, metabolic and endocrine traits in veal calves M. Rrat, Y. Zbinden, R. Saner, H. Hammon, and J.W. Blum Dairy farms with organic production (OP) and with integrated production (IP): comparison of management, feeding, production, reproduction and udder health M. Roesch, E. Homfeld, M.G. Doherr and J.W. Blum Milk production, nutritional status, and fertility in dairy cows held in organic farms and in farms with integrated production E. Homfeld, M. Roesch, M.G. Doherr and J.W. Blum Oviductal prolapse led to more than eleven percent of hens dead in a highly inbred line of white leghorn chickens H.M. Zhang, A.R. Pandiri and G.B. Kulkarni Section F. Epidemiology of production diseases Epidemiology of subclinical production diseases in dairy cows with an emphasis on ketosis 126 T.F. Duffield Associations of cow and management factors with culture positive milk samples R. van Dorp and D. Kelton 136 120

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A mathematical model for the dynamics of digital dermatitis in groups of cattle to study the efficacy of group-based therapy and prevention strategies D. Dopfer, M.R. van Boven, and M.C.M. de Jong Diagnostic test evaluation without gold standard using two different bayesian approaches for detecting verotoxinogenic Escherichia coli (VTEC) in cattle D. Dopfer, L. Geue, W. Buist and B. Engel A relative comparison of diagnostic tests for bovine paratuberculosis (Johnes disease) S.H. Hendrick, T.F. Duffield, D.F. Kelton, K.E. Leslie, K. Lissemore and M. Archambault Etiology, pathophysiology and prevention of fatty liver in dairy cows R.R. Grummer Relationships between mild fatty liver and health and reproductive performance in Holstein cows G. Bobe, B.N. Ametaj, R.A. Nafikov, D.C. Beitz and J.W. Young Prevention of fatty liver in transition dairy cows by subcutaneous glucagon injections R.A. Nafikov, B.N. Ametaj, G. Bobe, K.J. Koehler, J.W. Young and D.C. Beitz Clinical relevance and therapy of fatty liver in cows M. Frll, H. Bekele, D. Rchert, L. Jckel, U. Delling and Th. Wittek The inflammation could have a role in the liver lipidosis occurrence in dairy cows G. Bertoni, Trevisi Erminio, Calamari Luigi and Bionaz Massimo Effect of propylene glycol on fatty liver development and hepatic gluconeogenesis in periparturient dairy cows T. Rukkwamsuk, Apassara Choothesa, Sunthorn Rungruagn and Theo Wensing The report of classical swine fever in Romania between april 2001 and april 2003 C. Pascu, V. Herman and N. Catana Epidemiological survey on the downers in Korea H.R. Han, C.W. Lee, H.S. Yoo, B.K. Park, Bo Han and D. Kim Section G. Alternatives to growth promoting antibiotics Alternatives to antibiotic feed additives Chad R. Risley and John Lopez Continuous use of a dry disinfectant/antiseptic to enhance health and well being in food-animal production facilities Bud G. Harmon Bovine colostrum as an alternative to feed additive in weaning diet improves gut health of piglets I. Le Hurou-Luron, A. Huguet and J. Le Dividich

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Production responses from antimicrobial and rendered animal protein inclusions in swine starter diets B.V. Lawrence, S.A. Hansen and D. Overend Section H. Macromineral metabolism and production diseases

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Strategies for controlling hypocalcemia in dairy cows in confinement and pasture settings 182 Jorge M. Snchez and Jesse P. Goff Phosphorus digestion and metabolism in ruminants: applications to production disease and environmental considerations Ernst Pfeffer Prevalence of subclinical hypocalcemia in U.S. dairy operations R.L. Horst, J.P. Goff and B.J. McCluskey The effect of low phosphorus intake in early lactation on apparent digestibility of phosphorus and bone metabolism in dairy cows A. Ekelund, R. Sprndly and K. Holtenius Bone metabolism of milk goat and sheep during pregnancy and lactation A. Liesegang and Juha Risteli Influence of starvation on fermentation in bovine rumen fluid (in vivo) M. Hltershinken, A. Hhling, N. Holsten and H. Scholz Serum mineral concentrations and periparturient disease in Holstein dairy cows Robert J. Van Saun, Amy Todd and Gabriella Varga The relevance of hypophosphataemia in cows M. Frll, T. Sattler and M. Hoops Section I. Micromineral nutrition, metabolism and homeostasis Trace element nutriture and immune function Jerry W. Spears 226 188 215

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Expected changes of the selenium content in foods of animal origin at a change from inorganic to organic selenium compounds for supplementation of the diet of farm animals 236 B.G. Pehrson Long acting injectable remedies to prevent cobalt and selenium deficiencies in grazing lambs and calves N.D. Grace and S.O. Knowles Parenteral and oral selenium supplementation of weaned beef calves W.S. Swecker, Jr., R.K. Shanklin, K.H. Hunter, C.L. Pickworth, G. Scaglia, and J.P. Fontenot 238 240

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Selenium yeast prevents nutritional muscular degeneration (NMD) in nursing calves B.G. Pehrson

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Section J. Role of specific fatty acids in animal health, reproduction, and performance Using conjugated linoleic acids for healthier animal products and as a management tool M.A. McGuire, E.E. Mosley, S.A. Mosley, A. Nudda and A. Corato Fatty acid composition of phospholipids and levels of alpha-tocopherol, total antioxidative capacity and malondialdehyde in liver and muscular tissue after dietary supplementation of various fats in cattle J. Rehage, O. Portmann, R. Berning, M. Kaske, W. Kehler, M. Hoeltershinken, R. Duehlmeier, M. Coenen and H.-P. Sallmann A field sample study investigating possible indicators of undernutrition in cattle S. Agens, M.F. Heath, R.M. Nixon, J.M. Wilkinson and C.J.C. Phillips Section K. Mastitis Activation of immune cells in bovine mammary gland secretions by zymosan treated bovine serum K. Kimura and Jesse P. Goff 260 244

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The effect of milk yield at dry-off on the likelihood of intramammary infection at calving 262 P.J. Rajala-Schultz, K.L. Smith and J.S. Hogan Mastitis therapy for persistent Escherichia coli on a large dairy P.M. Sears, C.E. Ackerman, K.M. Crandall and W.M. Guterbock Diagnostic key data for lactate dehydrogenase activity measurements in raw milk for the identification of subclinical mastitis in dairy cows A. Neu-Zahren, U. Mller, S. Hiss and H. Sauerwein Evaluation of leukocyte subset for occurrence of mastitis on dairy herd H. Ohtsuka, M. Kohiruimaki, T. Hayashi, K. Katsuta, R. Abe and S. Kawamura Antimicrobial treatment strategies for Streptococcal and Staphylococcal mastitis K.D. Crandall, Philip M. Sears and Walter M. Guterbock Subclinical mastitis in dairy cows in farms with organic and with integrated production: prevalence, risk factors and udder pathogens M. Roesch, E. Homfeld, M.G. Doherr, W. Schaeren, M. Schllibaum, J.W. Blum Evaluation of a novel on-farm test for antibiotic susceptibility determination in mastitis pathogens B.C. Love and P. Rajala-Schultz 263

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Section L. Rumen digestion and metabolism Physically effective fiber and regulation of ruminal pH: more than just chewing M.S. Allen, J.A. Voelker and M. Oba High dietary cation difference induces a state of pseudohypoparathyroidism in dairy cows resulting in hypocalcemia and milk fever J.P. Goff and R.L. Horst Effects of two different dry-off strategies on metabolism in dairy cows M. Odensten, K. Person Waller and K. Holtenius Ruminal pH, concentrations and post-feeding pattern of VFA and organic acids in cows experiencing subacute ruminal acidosis G.R. Oetzel and K.M. Krause Characterization of the Na+/Mg2+ exchanger as a major Mg2+ transporter in isolated ovine ruminal epithelial cells M. Schweigel, B. Etschmann, F. Buschmann, H.S. Park and H. Martens High potassium diet, sodium and magnesium in ruminants: the story is not over F. Stumpff, I. Brinkmann, M. Schweigel and H. Martens Functional characterization of the time course of rumen epithelium adaptation to a high energy diet B. Etschmann, A. Suplie and H. Martens Characterization of an ovine vacuolar H+-ATPase as a new mechanism for the energization of ruminal transport processes M. Schweigel, B. Etschmann, E. Froschauer, S. Heipertz and H. Martens The absorptive capacity of sheep omasum is modulated by the diet O. Ali, H. Martens and C. Wegeler Section M. Application of genomics to production diseases Microarray analysis of bovine neutrophils around parturition: implications for mammary gland and reproductive tract health Jeanne L. Burton, Sally A. Madsen, Ling-Chu. Chang, Kelly R. Buckham, Laura E. Neuder and Patty S.D. Weber Functional genomics analysis of bovine viral diarrhea virus-infected cells: unraveling an enigma John D. Neill and Julia F. Ridpath 292 270

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Early postpartum ketosis in dairy cows and hepatic gene expression profiles using a bovine cDNA microarray 308 J.J. Loor, H.M. Dann, D.E. Morin, R.E. Everts, S.L. Rodriguez-Zas, H.A. Lewin and J.K. Drackley

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Genetic improvement of dairy cattle health J.B. Cole, P.D. Miller, and H.D. Norman Parturition-induced gene expression signatures in bovine peripheral blood mononuclear cells L-C. Chang, R. van Dorp, P.S.D. Weber, K.R. Buckham, and J.L. Burton Mechanisms of glucocorticoid-induced L-selectin (CD62L) down-regulation in bovine blood neutrophils Patty S.D. Weber, Ling-Chu. Chang, Trine Toelboell and Jeanne L. Burton

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T-cell receptor Vb gene repertoire analysis of the mammary gland T-cells on the Staphylococcus aureus causing bovine mastitis 312 T. Hayashi, H. Ohtsuka, M. Kohiruimaki, K. Katsuda, Y. Yokomizo, S. Kawamura and R. Abe A new sensitive microarray for studying metabolic diseases in cattle B.E. Etchebarne, W. Nobis, M.S. Allen, and M.J. VandeHaar Ontogenetic development of mRNA levels and binding sites of hepatic beta-adrenergic receptors in cattle J. Carron, H.M. Hammon, C. Morel and J.W. Blum Bovine lactase messenger RNA levels determined by real time PCR E.C. Ontsouka, B. Korczack, H.M. Hammon and J.W. Blum Design and application of a bovine metabolism long oligonucleotide microarray B.E. Etchebarne, W. Nobis, K.J. Harvatine, M.S. Allen, P.M. Coussens and M.J. VandeHaar Meeting summaries Meeting summary and synthesis: new scientific directions for production medicine research J.K. Drackley Meeting summary and synthesis: practical applications and new directions for applied production medicine research Walter M. Guterbock Author index 332 313

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