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Chapter 7
Summary
In the study involving radiotherapy of murine tumors, the scientific question being
addressed is that of tumor oxygenation, its relation to radiation therapy, and the ability to
such a study is that the behavior of tumors in response to radiation will allow for strategic
use of such therapies in the treatment of human cancers. During the course of these
experiments, we found that there was a discrepancy between the spectroscopic and
imaging data. My contribution to this study was in reconciling the differences between
the spectroscopic and imaging data, allowing for a direct comparison between these two
different modalities. This work was presented at the IEEE conference where it took first
place. This study was a collaboration between Drs. Sotak, Dardzinski, and myself.
The study involving the correlation between D(t) of tumor 1H2O and pO2 derived its
significance from the fact that it challenged an existing theory. While it is difficult to
challenge peer reviewed published work, this study showed on a larger scale that theories
evolve as information accumulates. Although this work was not the effort of one
individual, the insight of the principal investigator (Dr. Karl Helmer) and his direction in
the study proved to be rewarding. This set an example of the need for good direction and
SUMMARY 151
clear vision in science. I was the second author on this paper, and my contribution was in
the animal care, data acquisition, and part of the data processing.
On the surface, the study involving yeast cell suspensions appears to be a validation of a
method. Indeed, this is true, but the importance of these experiments lie in the
application of the method. Due to the complexity of interpreting data obtained from
animal experiments, it often becomes necessary to take a step back and find an analogous
model to answer questions arising from the animal experiments. Specifically, the
question that vexed us was the diffusion behavior of water in a two-compartment system.
During cerebral ischemia, the brain water apparent diffusion coefficient (ADC) decreases,
and there are many plausible explanations behind this phenomena. Others have done
studies involving intra- and extracellular metabolites to infer the behavior of the water
diffusion coefficient from the behavior of these metabolites, but it is not evident a priori
that the diffusion characteristics of these metabolites track those of water. Not only so,
but metabolites that are constrained to only one compartment cannot accurately represent
water, which can move between the two compartments. This study was a stepping stone
to answering the perplexing question of what is really happening to the brain water at the
onset of ischemia. I was responsible for working out the specific theory behind the
experiments, designing the experiments, writing the pulse sequences, acquiring the data,
and analyzing the data. Although this work was a collaborative effort of many, I was the
one who set the direction of these experiments and analyzed the data to answer the
underlying question of compartmental 1H2O ADC behavior. The future direction of this
SUMMARY 152
particular study would be to further characterize the water behavior in yeast by measuring
the time-dependence of the ADC to extract structural parameters from the cellular
the rat brain under different pathological conditions would need to be done.
The rabbit Achilles tendon experiment showed the true multi-faceted nature of some
NMR studies. The characterization of a system that is not well known is not a trivial
task, and in this case, it was especially necessary to collaborate with scientists from
engineers, and NMR scientists were all involved in every step of these experiments.
Although many people collaborated in this study, I was the one who set the direction for
these studies. Since the system was not well characterized, there was a need to narrow
the overall scope of this study and specify which questions needed to be addressed. I was
responsible for forming the main scientific questions that needed to be addressed and
working out the specific experiments necessary to characterize the system. I also wrote
the pulse sequences necessary for the data acquisition and wrote the code to analyze all of
the data. Since this study involving the rabbit Achilles tendon is fairly new, there is
much that can be done with this and also many different directions in which this study
can go. One point of interest is that the behavior of visco-elastic solids is different for
dynamic loading conditions that for static loading conditions. The study of the water
due to the highly structured fiber orientation of the tendon, it would be interesting to
know if there are any directional susceptibility artifacts that manifest themselves as a