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R1R1 cell pools C,D and E

Cell pools made 12/3/09 Test date 12/3/09


Standard AHG technique all dilutions PBSS

Anti-D 73/515
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Pool C 4 3 3 1 0 0
Pool D 4 3 2 0 0 0
Pool E 4 3 2 1 0 0

Anti-D 86 i.u.
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Pool C 3 3 1 0 0 0
Pool D 3 2 1 0 0 0
Pool E 3 2 1 0 0 0

Anti-D lot 24311


Diltuion 1:4 1:8 1:16 1:32 1:64 1:128
Pool C 3 3 2 1 0 0
Pool D 3 2 1 0 0 0
Pool E 3 2 1 0 0 0

Anti-D Lot 32372B


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Pool C 4 3 2 1 0 0
Pool D 4 3 2 1 0 0
Pool E 4 3 2 1 0 0

Anti-D Lot 101


Dilution 1:50 1:100 1:200 1:400 1:800 1:1600
Pool C 2 1 0 0 0 0
Pool D 2 1 0 0 0 0
Pool E 2 1 0 0 0 0

Anti-D Patient Pool A


Dilution Neat 1:2 1:4 1:8 1:16 1:32
Pool C
Pool D
Pool E

Anti-D Lot 102


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Pool C 3 2 1 0 0 0
Pool D 2 1 0 0 0 0
Pool E 3 2 1 0 0 0
Anti-D Lot 201
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Pool C 3 2 1 0 0 0
Pool D 3 2 0 0 0 0
Pool E 3 2 1 0 0 0

Anti-D Lot 202


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Pool C 1 0 0 0 0 0
Pool D 1 0 0 0 0 0
Pool E 1 0 0 0 0 0

Anti-D Lot 103


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Pool C 3 2 1 0 0 0
Pool D 3 2 1 0 0 0
Pool E 3 2 1 0 0 0

Anti-D Lot 203


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Pool C 4 3 2 1 0 0
Pool D 4 3 2 1 0 0
Pool E 4 4 2 1 0 0

Anti-D Patient Pool B


Dilution Neat 1:2 1:4 1:8 1:16 1:32
Pool C 1 1 1 1 1 1
Pool D 1 1 1 1 1 1
Pool E 2 1 0 0 0 0
R1R1 cell pools C,D and E
Cell pools made 12/3/09 Test date 16/3/09
Standard AHG technique all dilutions PBSS

Anti-D 73/515
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Pool C 3 2 1 0 0 0
Pool D 3 2 1 0 0 0
Pool E 3 3 2 1 0 0

Anti-D 86 i.u.
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Pool C 4 2 1 0 0 0
Pool D 4 2 1 0 0 0
Pool E 4 2 1 0 0 0

Anti-D lot 24311


Diltuion 1:4 1:8 1:16 1:32 1:64 1:128
Pool C 3 2 1 0 0 0
Pool D 3 2 0 0 0 0
Pool E 3 2 1 0 0 0

Anti-D Lot 32372B


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Pool C 4 3 2 1 0 0
Pool D 4 3 2 1 0 0
Pool E 4 3 2 1 0 0

Anti-D Lot 101


Dilution 1:50 1:100 1:200 1:400 1:800 1:1600
Pool C 2 1 0 0 0 0
Pool D 2 1 0 0 0 0
Pool E 2 1 0 0 0 0

Anti-D Patient Pool A


Dilution Neat 1:2 1:4 1:8 1:16 1:32
Pool C MF MF MF 1 1 1
Pool D MF 3 3 2 1 0
Pool E 1 1 0 0 0 0

Anti-D Lot 102


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Pool C 2 1 0 0 0 0
Pool D 2 1 0 0 0 0
Pool E 2 1 0 0 0 0
Anti-D Lot 201
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Pool C 3 2 1 0 0 0
Pool D 2 1 0 0 0 0
Pool E 3 2 1 0 0 0

Anti-D Lot 202


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Pool C 1 0 0 0 0 0
Pool D 1 0 0 0 0 0
Pool E 1 0 0 0 0 0

Anti-D Lot 103


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Pool C 3 2 1 0 0 0
Pool D 3 2 1 0 0 0
Pool E 3 2 1 0 0 0

Anti-D Lot 203


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Pool C 3 3 2 1 0 0
Pool D 3 3 2 1 0 0
Pool E 4 3 2 1 0 0

Anti-D Patient Pool B


Dilution Neat 1:2 1:4 1:8 1:16 1:32
Pool C 3 2 2 2 2 1
Pool D 3 2 2 2 1 0
Pool E 2 0 0 0 0 0
R1R1 cell pools C,D and E
Cell pools made 12/3/09 Test date 17/3/09
Standard AHG technique all dilutions PBSS

Anti-D 73/515
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Pool C 3 2 1 0 0 0
Pool D 3 2 1 0 0 0
Pool E 3 2 1 0 0 0

Anti-D 86 i.u.
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Pool C 3 2 1 0 0 0
Pool D 3 2 1 0 0 0
Pool E 3 2 1 0 0 0

Anti-D lot 24311


Diltuion 1:4 1:8 1:16 1:32 1:64 1:128
Pool C 3 3 2 1 0 0
Pool D 3 3 2 1 0 0
Pool E 3 3 2 1 0 0

Anti-D Lot 32372B


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Pool C 2 2 1 0 0 0
Pool D 3 2 1 0 0 0
Pool E 3 2 1 0 0 0

Anti-D Lot 101


Dilution 1:50 1:100 1:200 1:400 1:800 1:1600
Pool C 2 2 1 0 0 0
Pool D 2 1 0 0 0 0
Pool E 2 1 1 0 0 0

Anti-D Patient Pool A


Dilution Neat 1:2 1:4 1:8 1:16 1:32
Pool C MF MF MF MF MF MF
Pool D 2 2 2 2 1 0
Pool E 1 0 0 0 0 0

Anti-D Lot 102


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Pool C 2 1 0 0 0 0
Pool D 2 1 0 0 0 0
Pool E 2 1 0 0 0 0
Anti-D Lot 201
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Pool C 3 2 1 0 0 0
Pool D 3 2 1 0 0 0
Pool E 3 2 1 0 0 0

Anti-D Lot 202


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Pool C 1 0 0 0 0 0
Pool D 1 0 0 0 0 0
Pool E 1 0 0 0 0 0

Anti-D Lot 103


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Pool C 3 2 1 0 0 0
Pool D 3 2 1 0 0 0
Pool E 3 2 1 0 0 0

Anti-D Lot 203


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Pool C 4 3 2 1 0 0
Pool D 4 3 2 1 0 0
Pool E 3 2 2 1 0 0
R1R1 cell pools C,D and E
Cell pools made 12/3/09 Test date 31/3/09
Standard AHG technique all dilutions PBSS

Anti-D 73/515
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Pool C 3 2 1 0 0 0
Pool D 3 2 1 0 0 0
Pool E 3 2 1 0 0 0

Anti-D 86 i.u.
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Pool C 3 2 1 0 0 0
Pool D 3 2 1 0 0 0
Pool E 3 2 1 0 0 0

Anti-D Lot 101


Dilution 1:50 1:100 1:200 1:400 1:800 1:1600
Pool C 2 1 0 0 0 0
Pool D 2 1 0 0 0 0
Pool E 2 1 0 0 0 0

2 RADDP Patients

RADDP Patient 1
Dilution Neat 1:2 1:4 1:8 1:16 1:32
Pool C 0 0 0 0 0 0
Pool D 0 0 0 0 0 0
Pool E 0 0 0 0 0 0

RADDP Patient 2
Dilution Neat 1:2 1:4 1:8 1:16 1:32
Pool C MF MF 1 1 1 1
Pool D 2 1 1 0 0 0
Pool E 0 0 0 0 0 0