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DIMETHYLGLYOXIME(DMG)
Nickel (II) was estimated in sample by the reaction between nickel and
Dimethylglyoxime (DMG); forming the Ni-DMG pink color complex on the TLC foils. It
was done by taking spots of samples (2µL of each concentration) with help of
micropipette on the TLC foils impregnated with 1% DMG.Each spot was scanned using
“Flat-bed scanner”. Density of each concentration colored spot was taken by
“Densitometry” software programmed in PC.Ten spots were taken for each concentration
and reproducibility of each concentration was determined by plotting reproducibility
curve .RSD and 95% confidence interval (95%C.I ) of each concentration was noted. The
data obtained by each concentration is given below.
ESTIMATION OF NICKEL (II) WITH DIMETHYLGLYOXIME
For 100 ppm of Ni (II) Sample
Cons
Serial # (ppm) Optical density
1 100 109.8
2 100 109.9
3 100 109
4 100 109.2
5 100 108
6 100 111
7 100 109.8
8 100 109.09
9 100 112.09
10 100 112
Mean =109.98
RSD = 1.209 %
95% C.I=109.98±0.6651
114
112
optical density
110
108
106
104
1 2 3 4 5 6 7 8 9 10
no of obs
Mean = 105.0668
RSD=0.668%
95% C.I=105.0668±0.3451
Reproducibility curve of 95 ppm conc
of Ni(II)sample
108
optical density
106
104
102
1 2 3 4 5 6 7 8 9 10
no of obs
102
optical density
101
100
99
98
1 2 3 4 5 6 7 8 9 10
no of obs
95
optical density
94.5
94
93.5
93
1 2 3 4 5 6 7 8 9 10
no of obs
92
optical density
90
88
86
1 2 3 4 5 6 7 8 9 10
no of obs
85
optical density
84
83
82
81
80
1 2 3 4 5 6 7 8 9 10
no of obs
For 70ppm of Ni (II) sample
Serial # Conc.(ppm) optical density
1 70 78.02
2 70 78.08
3 70 78.5
4 70 79.07
5 70 78
6 70 78.09
7 70 77.9
8 70 78.05
9 70 77
10 70 77.32
Mean=78.003
RSD=0.726%
95%C.I=78.003±0.2831
80
optical density
79
78
77
76
75
1 2 3 4 5 6 7 8 9 10
no of obs
73
72.5
optical density
72
71.5
71
70.5
70
1 2 3 4 5 6 7 8 9 10
no of obs
67.5
67
optical density
66.5
66
65.5
65
64.5
64
63.5
1 2 3 4 5 6 7 8 9 10
no of obs
For 55ppm of Ni (II) sample
Serial # conc.(ppm) optical density
1 55 60
2 55 60.87
3 55 61.9
4 55 62
5 55 62
6 55 59
7 55 60.4
8 55 62.8
9 55 62
10 55 60.8
Mean=61.2
RSD=1.899%
95%C.I=61.2±0.581
64
optical density
62
60
58
56
1 2 3 4 5 6 7 8 9 10
no of obs
52
51
optical density
50
49
48
1 2 3 4 5 6 7 8 9 10
no of obs
46
optical density
45
44
43
1 2 3 4 5 6 7 8 9 10
no of obs
45
optical density
40
35
30
1 2 3 4 5 6 7 8 9 10
no of obs
For 30 ppm of Ni (II) sample
35
optical density
34
33
32
31
1 2 3 4 5 6 7 8 9 10
no of obs
32
optical density
30
28
26
24
1 2 3 4 5 6 7 8 9 10
no of obs
23.5
23
optical density
22.5
22
21.5
21
20.5
1 2 3 4 5 6 7 8 9 10
no of obs
18
Optical density
17.5
17
16.5
16
15.5
15
1 2 3 4 5 6 7 8 9 10
no of obs
20
optical density
15
10
0
1 2 3 4 5 6 7 8 9 10
no of obs
120
R2 = 0.9998
100
80
optical density
60
40
20
0
10
20
30
40
50
60
70
80
90
0
10
Concentration (ppm )