Spectrophotometric BROMOCRESOL GREEN The application parameters comprised here constitute a guide to facilitate the validation of our reagents

by the instrument. It is advisable to validate the use when there is any change in software or reagent versions.

ALBUMIN

Instrument:
Reagent preparation
Reagent is ready to use.

RX-DAYTONA (=BQA)

Instrument settings
CHEMISTRY PARAMETERS Name Unit Assay Type Measuring Points 1 2 Wavelength Prim. Sampling Volume Rerun CALIBRATION Calculation 660 3 L 2 L Linear ALB g/L Endpoint 5 R1 R2 Reagent Name Reagent Name ALB Volume 300 L

Start End Start End

Sec.

Decimal points 0 Normal Range (Male) 35 (Female) 35 (Child) 28 Technical Range (Conc.) 1.4 (mAbs/10) -35000 RPT Wash (R1) Sys Water (R2) Sys Water 0 Instrument Factor Stirring Speed

50 50 54 - 70 - 35000 Wash Sol.-3 Wash Sol.-3 b 0 R2 Mid

S1 S2

a 1 R1 Mid

... Data entered by the operator

Vers. 0510

DIRECT BILIRUBIN

Spectrophotometric DIAZOTIZED SULFANILIC

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument:
Reagent preparation

RX-DAYTONA (=BQA)

Working Reagent: Transfer the contents of one Reagent B vial into a Reagent A-D bottle. Mix thoroughly. Stable for 20 days at 2-8 C.

Instrument settings
CHEMISTRY PARAMETERS Name Unit Assay Type Measuring Points 1 2 Wavelength Prim. Sampling Volume Rerun CALIBRATION Calculation 546 20 L 10 L Linear BIL-D mg/dL Endpoint R1 R2 Reagent Name Reagent Name BIL-D BIL-D Volume 200 L Volume 50 L

Start End Start End

23 Sec.

Decimal points 2 Normal Range (Male) 0.00 (Female) 0.00 (Child) Technical Range (Conc.) 0.00 (mAbs/10) -35000 RPT Wash (R1) Sys Water (R2) Sys Water 0 Instrument Factor Stirring Speed

- 0.25 - 0.25 - - 15.00 - 35000 Wash Sol.-3 Wash Sol.-3 b 0 R2 Mid

S1 S2

a 1 R1 Mid

... Data entered by the operator

Vers. 0510

TOTAL BILIRUBIN

Spectrophotometric DIAZOTIZED SULFANILIC

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument:
Reagent preparation

RX-DAYTONA (=BQA)

Working Reagent: Transfer the contents of one Reagent B vial into a Reagent A-T bottle. Mix thoroughly. Stable for 20 days at 2-8 C.

Instrument settings
CHEMISTRY PARAMETERS Name Unit Assay Type Measuring Points 1 2 Wavelength Prim. Sampling Volume Rerun CALIBRATION Calculation 546 20 L 2 L Linear BIL-T mg/dL Endpoint R1 R2 Reagent Name Reagent Name BIL-T BIL-T Volume 200 L Volume 50 L

Start End Start End

23 Sec.

Decimal points 2 Normal Range (Male) 0.00 (Female) 0.00 (Child) Technical Range (Conc.) 0.00 (mAbs/10) -35000 RPT Wash (R1) Sys Water (R2) Sys Water 0 Instrument Factor Stirring Speed

- 1.10 - 1.10 - - 15.00 - 35000 Wash Sol.-3 Wash Sol.-3 b 0 R2 Mid

S1 S2

a 1 R1 Mid

... Data entered by the operator

Vers. 0510

CREATINE KINASE-MB
Inmunoinhibition The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument:
Reagent preparation

RX-DAYTONA (=BQA)

Working Reagent: Reconstitute the contents of a Reagent B vial with 2.5 mL (if 20x2.5 mL size) or 10 mL (if 10x10 mL size) of Reagent A. Mix gently. Stable for 15 days at 2-8C.

Instrument settings
CHEMISTRY PARAMETERS Name Unit Assay Type Measuring Points 1 2 Wavelength Prim. Sampling Volume Rerun CALIBRATION Calculation 340 10 L 5 L Linear CK-MB U/L Kinetic 21 30 R1 R2 Reagent Name Reagent Name CK-MB Volume 250 L

Start End Start End

Sec.

Decimal points 0 Normal Range (Male) 0 (Female) 0 (Child) 0 Technical Range (Conc.) 0 (mAbs/10) -35000 RPT Wash (R1) Sys Water (R2) Sys Water 0 Instrument Factor Stirring Speed a 1 R1 Mid

24 24 24 330 35000 Wash Sol.-3 Wash Sol.-3 b 0 R2 Mid

S1 S2

... Data entered by the operator

Vers. 0510

LACTATE DEHYDROGENASE
Continuous-spectrophotometric PYRUVATE The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument:
Reagent preparation

RX-DAYTONA (=BQA)

Working Reagent: Pour the contents of the Reagent B into the Reagent A bottle. Mix gently. Stable for 2 months at 2-8C.

Instrument settings
CHEMISTRY PARAMETERS Name Unit Assay Type Measuring Points 1 2 Wavelength Prim. Sampling Volume Rerun CALIBRATION Calculation 340 5 L 2 L Linear LDH U/L Kinetic 2 11 R1 R2 Reagent Name Reagent Name LDH Volume 300 L

Start End Start End

Sec.

Decimal points 0 Normal Range (Male) 207 (Female) 207 (Child) 207 Technical Range (Conc.) 0 (mAbs/10) -35000 RPT Wash (R1) Sys Water (R2) Sys Water 0 Instrument Factor Stirring Speed a 1 R1 Mid

414 414 414 1500 35000 Wash Sol.-3 Wash Sol.-3 b 0 R2 Mid

S1 S2

... Data entered by the operator

Vers. 0510

Spectrophotometric BIURET

PROTEIN

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument:
Reagent preparation
Reagent is ready to be used.

RX-DAYTONA (=BQA)

Instrument settings
CHEMISTRY PARAMETERS Name Unit Assay Type Measuring Points 1 2 Wavelength Prim. Sampling Volume Rerun CALIBRATION Calculation 546 6 L 3 L Linear PROT g/L Endpoint 30 R1 R2 Reagent Name Reagent Name PROT Volume 300 L

Start End Start End

Sec.

Decimal points 0 Normal Range (Male) 65 (Female) 65 (Child) 0 Technical Range (Conc.) 0 (mAbs/10) -35000 RPT Wash (R1) Sys Water (R2) Sys Water 0 Instrument Factor Stirring Speed a 1 R1 Mid

83 83 150 800 35000 Wash Sol.-3 Wash Sol.-3 b 0 R2 Mid

S1 S2

... Data entered by the operator

Vers. 0510