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[2m]
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[AP 2010]
2 A dipeptide was heated with an acid for 20 minutes. Three different samples were taken and
loaded on to a piece of chromatography paper. These were:
I
the peptide only;
solvent
II the dipeptide after it had been heated with the acidfor
front
2 minutes;
III the dipeptide after it had been heated with the acidfor
spot X
20 minutes.
The resulting chromatogram is shown in the drawing.
(a) What type of reaction results in the conversion of a dipeptide
to amino acids?
[1 m]
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(b) Explain why three spots were obtained when the dipeptide
was heated with the acid for 2 minutes.
[2 m]
II
III
_________________________________________________________________
II
(c) (i) Calculate the Rf value of spot X. Show your steps
(ii) Explain how you could use the Rf values to identify spot X.
[2 m]
[1 m]
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__________________________________________________________________________________________________________
Bio1TC2Chromatography QA
sugars.
(i) The Rf value of maltose is 0.25.
Mark on the diagram the position of
the spot representing maltose.
[1 m]
(ii) Which of A, B, C or D represents the
complete hydrolysis of sucrose? [1 m]
.............................................................
(iii) The spots from mixture D were very
close together. What could you do to
a chromatogram of mixture D to
separate the spots further?
[2 m]
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AQA BYA1 Jan 06
4 (a)
[2 m]
(b) Use the appropriate letters from A, B, C, D and E to write a simple equation to
show how the Rf value
of the sugar in spot S could be calculated.
[1 m]
AQA BYA1 Jan 04
Rf =
Answers
TAH
Bio1TC2Chromatography QA
1(a)
(i)
Correct position indicated; 1
(ii) C; 1
(iii) Turn through 90/ two-way chromatography/2D;
Run in a different solvent; 2
4 (a) (i) Ink will dissolve in solvent/will obscure spots (as it rises)/ink will run;
(Look for principle of interfering with chromatogram) 1
(ii) Repeated placing of drops;
Allowing to dry between applications; 2
(b) C .
(B + C) ;
TAH