Adam M.

Blattler
Farnham Lab, University of Southern California 1450 Biggy Street, Los Angeles CA 90089 Norris Research Tower (NRT) 6514 adam.blattler@gmail.com 700 Orange Grove Avenue Apt. 101 South Pasadena, CA 91030 (925) 998-3756

Education PhD Candidate, Genetics Graduate Group, University of California, Davis, September 2008 present, Advisor: Peggy J. Farnham, Cumulative GPA: 4.0 Bachelor of Science, Genetics, University of California, Davis, Graduated with Honors, June 2008 Alhambra High School, Martinez, CA, Class of 2004 Areas of Specialization Epigenomic cancer research Chromatin immunoprecipitation Next Generation Sequencing: ChIP-seq, RNA-seq, Whole genome bisulfite-seq Protein biochemistry and molecular cloning siRNA and shRNA knockdown of gene expression and qPCR Human tissue culture, including work with human embryonic stem cells Next Generation sequencing analysis: ChIP-seq, RNA-seq, DNA methylation data Software: HOMER, bedtools, SAMtools, Bowtie, Tophat, Cufflinks, Cluster3, Java TreeView, Perl, Unix

Publications Blattler A, Yao L, Wang Y, Ye Z, Jin V, and Farnham PJ. ZBTB33 binds unmethylated regions of the genome associated with actively expressed genes. Epigenetics and Chromatin, Highly Accessed, May 2013 Wang R, Hsu HK, Blattler A, Wang Y, Lan X, Wang Y, Hsu PY, Leu YW, Farnham PJ, Huang T, Jin V. LOcating Non-Unique matched Tags (LONUT) to improve the detection of the enriched regions for ChIPseq data. PLoS ONE, June 2013 Awards and Fellowships Ruth L. Kirschstein National Research Service Awards for Individual Predoctoral Fellowships (F31) , University of California Davis. June 2010 June 2014 Carl Storm Underrepresented Minority (CSURM) Fellowship for travel to the Gordon Research Conference for Cancer Genetics and Epigenetics in Lucca, Italy. December 2012 NIH Molecular and Cellular Biology Training Grant Fellowship, University of California Davis. June 2010 June 2011. Graduate Student Travel Award, University of California, Davis. December 2009 Undergraduate Citation Award for Outstanding Academic and Research Achievements in Molecular and Cellular Biology, University of California, Davis. May 2008 Work and Research Experience Graduate Student Researcher, Farnham Lab, University of Southern California, April 2011 Present. I am investigating the function and genome-wide DNA-binding properties of transcription factors as it relates to global DNA methylation patterning and cancer. Specifically, my dissertation project aims to study the role of POZ and BTB domain-containing zinc finger proteins (ZBTBs) and their role in the epigenetic regulating the genome in the presence and absence of DNA. I am interested in the epigenetic regulation of the genome, and how the methyl-DNA landscape of the human genome dictates transcription factor binding and recruitment of histone-modifying proteins. Graduate Student Researcher, Farnham Lab, UC Davis, Spring 2009 March 2011 Investigation of the function and DNA-binding properties of transcription factors, specifically zinc finger proteins, their evolution in higher mammals, and their role in human disease. Summer Intern at Bio-Rad Laboratories, Summer 2008 I worked with the Gene Expression Division Research and Development group on the development and

validation of their siRNA product line. Work included extensive tissue culture and the qPCR analysis of gene expression in cells transfected with different siRNA constructs. The internship included a 15-minute Power Point presentation to Senior Management and co-workers. Undergraduate Researcher, Britt Lab, September 2007 June 2008 Research included transformations of the model organism Arabidopsis using binary vectors in Agrobacterium as a vehicle for gene replacement or transformation. I worked on finding a more efficient way to select for the homologous integration of a gene construct over the more common non-homologous end-joining pathway. Summer Intern at Bio-Rad Laboratories, Summer 2007 I worked with the Gene Expression Division Marketing Group with Product Management. Responsibilities included detailed market competitive analyses, research (qPCR/qRT-PCR and cell culture work), and creation of technical bulletins. It also included a 15-minute Power Point presentation to Senior Management and co-workers.

Presentations, Academic Services, and Professional Societies Poster Presentations: Adam Blattler and Peggy J Farnham; Sequence-specific DNA-binding of Evolutionarily Divergent Zinc Finger Proteins, Meeting on Mechanisms of Eukaryotic Transcription, Cold Spring Harbor, NY. August 2009 Adam Blattler, Lijing Yao, Peggy J. Farnham; In Vivo Insights on the Genome-Wide Binding Patterns of Kaiso, a Methyl-Sensitive Zinc Finger Transcription Factor . University of Southern California Department of Biochemistry and Molecular Biology Retreat. Lake Arrowhead, CA. March 2013 Adam Blattler, Lijing Yao, Yao Wang, Zhenqing Ye, Victor X. Jin, and Peggy J. Farnham; Kaiso binds unmethylated regions of the genome associated with actively expressed genes ; The Gordon Research Conference on Cancer Genetics and Epigenetics; Barga, Italy April 2013 Adam Blattler, Lijing Yao, Yao Wang, Zhenqing Ye, Victor X. Jin, and Peggy J. Farnham; Kaiso binds unmethylated regions of the genome associated with actively expressed genes ; The ENCODE Consortium Meeting, Stanford University, May 2013 Adam Blattler, Lijing Yao, Yao Wang, Zhenqing Ye, Victor X. Jin, and Peggy J. Farnham; Kaiso binds unmethylated regions of the genome associated with actively expressed genes ; The Norris Comprehensive Cancer Center 40th Anniversary Symposium, University of Southern California, October 2013 Academic Services: Genetics Graduate Group Student Executive Committee Chair . University of California, Davis (Fall 2010 Spring 2011) Genetics Graduate Group Student Recruitment Committee member. University of California, Davis (Fall 2009 Spring 2011) Co-founder of the Zinc Finger Journal Club. University of California, Davis (Winter 2009 Winter 2010) Professional Societies: Phi Sigma Biological Sciences Honor Society Member. (May 2010 Present) AAAS Member (March 2011 Present) Active contributing member of the Encyclopedia of DNA Elements (ENCODE) Consortium as a member of the Farnham Lab Meetings Attended without poster presentations ENCODE Consortium Meeting. Stanford University, Palo Alto, CA. May 2012 Future of Genomic Medicine, Scripps Seaside Forum, La Jolla, CA. March 2013 ENCODE Consortium Meeting. Crystal City, VA. May 2011 ENCODE Consortium Meeting. Bethesda, MD. March 2010 Teaching and Mentoring Teaching Assistant, GGG201C: Graduate Level Molecular Genetics. University of California Davis Mentoring: Yu Guo, Masters student in the Farnham Lab. DNA Methylation and Transcription Factor Binding, meeting once per week.

Two-hour Lecture: Graduate class on the Statistical Analysis of High-dimensional DNA . Title: Chromatin immunoprecipitation Experimental procedures and downstream analyses. University of Southern California, August 2013.

Ongoing Collaborations Peter Jones, University of Southern California, Norris Comprehensive Cancer Center Victor Jin, Ohio State University Department of Biomedical Informatics Kim Siegmund, University of Southern California Department of Preventative Medicine Ruchi Bajpai, University of Southern California Department of Biochemistry and Molecular Biology Research Support NIH/NHGRI F31 HG006114-01 (Blattler: PI) 07/10-06/14 Sequence-specific DNA-binding of evolutionary divergent KRAB-zinc finger proteins The aims of this project are: (Aim 1) to test the hypothesis that divergence in the zinc finger domains dictates different binding capabilities of 10 highly related human and one mouse KRAB-ZNFs and (Aim 2) to test the hypothesis that highly related KRAB-ZNFs regulate different functional categories of genes. References (Contact information available upon request) Peggy J Farnham, PhD: William M Keck Professor of Biochemistry, Norris Comprehensive Cancer Center,Associate Dean of Graduate AffairsKeck School of Medicine, University of Southern California David J Segal, PhD: Associate Professor, Associate Director of Genomics, Department of Biochemistry and Molecular Medicine, School of Medicine, University of California Davis Frdric Chedin, PhD: Associate Professor, Department of Molecular and Cellular Biology, University of California Davis Benjamin Berman, PhD: Assistant Professor, Division of Bioinformatics, Department of Preventive Medicine, University of Southern California Seth Frietze, PhD: Assistant Professor, Department of Biological Sciences, University of Northern Colorado Kamni Vijay, PhD: Marketing Director, Bio-Rad Laboratories.